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characterization of glycoside hydrolase 134 family mannanase

Research Project

Project/Area Number 17K07734
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionMeijo University

Principal Investigator

Shimizu Motoyuki  名城大学, 農学部, 准教授 (20423535)

Co-Investigator(Kenkyū-buntansha) 佐藤 育男  名古屋大学, 生命農学研究科, 助教 (70743102)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsβ-1,4-mannanase / AoMan134 / β-mannan / GH 134 / mannnooligosaccharide / Aspergillus oryzae / β-マンナナーゼ / GH134 / Aspergillus nidulans / Man134 / 構造解析 / 酵素 / 菌類 / 微生物 / マンナナーゼ
Outline of Final Research Achievements

We investigated the enzymatic function and structure of β-1,4-mannanase AoMan134A from Aspergillus oryzae. Recombinant AoMan134A was prepared, and the reaction products from galactose-free β-mannan generated by the enzyme were analyzed. AoMan134A released mannobiose (M2), mannotriose (M3), and mannotetraose (M4) but not mannopentaose (M5) or higher manno-oligosaccharides when galactose-free β-mannan was the substrate. AoMan134A had high catalytic efficiency (kcat/Km) towards M6 compared with the endo-β-1,4-mannanase Man5C and notably converted M6 to M2, M3, and M4, with M3 being the predominant reaction product. We also revealed that AoMan134A had lyzozyme-like structure with two active-site amino acid residues.

Academic Significance and Societal Importance of the Research Achievements

AoMan134Aは、熱安定性を有する既知のβ-マンナナーゼと同等もしくはそれ以上の高い熱安定性をもち、中性からアルカリ性においてpH 安定性を有していた。現在産業利用されている既存のβ-マンナナーゼと比較して、高い熱安定性および効率的なマンノオリゴ糖生産能を有していることから、AoMan134Aは機能性マンノオリゴ糖の生産に適していると考えられ、マンノオリゴ糖を含む(食品)産業での応用が期待できる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (24 results)

All 2020 2019 2018 2017

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 5 results) Presentation (15 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results)

  • [Journal Article] Oxygen radical based on non-thermal atmospheric pressure plasma alleviates lignin-derived phenolic toxicity in yeast2020

    • Author(s)
      Ito Shou、Sakai Kiyota、Gamaleev Vladislav、Ito Masafumi、Hori Masaru、Kato Masashi、Shimizu Motoyuki
    • Journal Title

      Biotechnology for Biofuels

      Volume: 13 Issue: 1 Pages: 18-18

    • DOI

      10.1186/s13068-020-1655-9

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ability of Saccharomyces cerevisiae MC87-46 to assimilate isomaltose and its effects on sake taste2019

    • Author(s)
      Tsutsumi Seitaro、Mochizuki Mai、Sakai Kiyota、Ieda Akane、Ohara Reiji、Mitsui Shun、Ito Akitoshi、Hirano Tatsuya、Shimizu Motoyuki、Kato Masashi
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 13908-13908

    • DOI

      10.1038/s41598-019-50384-w

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification and characterization of a thermostable pectate lyase from Aspergillus luchuensis var. saitoi2019

    • Author(s)
      Kamijo Junya、Sakai Kiyota、Suzuki Hiromitsu、Suzuki Kengo、Kunitake Emi、Shimizu Motoyuki、Kato Masashi
    • Journal Title

      Food Chemistry

      Volume: 276 Pages: 503-510

    • DOI

      10.1016/j.foodchem.2018.10.059

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Biochemical Characterization of CYP505D6, a Self-Sufficient Cytochrome P450 from the White-Rot Fungus Phanerochaete chrysosporium2018

    • Author(s)
      Sakai Kiyota、Matsuzaki Fumiko、Wise Lisa、Sakai Yu、Jindou Sadanari、Ichinose Hirofumi、Takaya Naoki、Kato Masashi、Wariishi Hiroyuki、Shimizu Motoyuki
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 84 Issue: 22

    • DOI

      10.1128/aem.01091-18

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of pH-tolerant and thermostable GH 134 β-1,4-mannanase SsGH134 possessing carbohydrate binding module 10 from Streptomyces sp. NRRL B-244842018

    • Author(s)
      Sakai Kiyota、Kimoto Saran、Shinzawa Yuta、Minezawa Miho、Suzuki Kengo、Jindou Sadanari、Kato Masashi、Shimizu Motoyuki
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 125 Issue: 3 Pages: 287-294

    • DOI

      10.1016/j.jbiosc.2017.10.009

    • NAID

      40021507480

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] NAD+/NADH homeostasis affects metabolic adaptation to hypoxia and secondary metabolite production in filamentous fungi2018

    • Author(s)
      Shimizu Motoyuki
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 82 Issue: 2 Pages: 216-224

    • DOI

      10.1080/09168451.2017.1422972

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Oxygen-radical pretreatment promotes cellulose degradation by cellulolytic enzymes2017

    • Author(s)
      Sakai Kiyota、Kojiya Saki、Kamijo Junya、Tanaka Yuta、Tanaka Kenta、Maebayashi Masahiro、Oh Jun-Seok、Ito Masafumi、Hori Masaru、Shimizu Motoyuki、Kato Masashi
    • Journal Title

      Biotechnology for Biofuels

      Volume: 10 Issue: 1 Pages: 290-290

    • DOI

      10.1186/s13068-017-0979-6

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Sirtuin E is a fungal global transcriptional regulator that determines the transition from the primary growth to the stationary phase2017

    • Author(s)
      Itoh Eriko、Odakura Rika、Oinuma Ken-Ichi、Shimizu Motoyuki、Masuo Shunsuke、Takaya Naoki
    • Journal Title

      Journal of Biological Chemistry

      Volume: 292 Issue: 26 Pages: 11043-11054

    • DOI

      10.1074/jbc.m116.753772

    • NAID

      120007128935

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Biochemical characterization of thermostable β-1,4-mannanase belonging to the glycoside hydrolase family 134 from Aspergillus oryzae2017

    • Author(s)
      Kiyota SakaiMai MochizukiMiyuki YamadaYuta ShinzawaMiho MinezawaSaran KimotoShunsuke MurataYuhei KanekoSaaya IshiharaSadanari JindouTetsuo KobayashiMasashi KatoMotoyuki Shimizu
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 101 Issue: 8 Pages: 3237-3245

    • DOI

      10.1007/s00253-017-8107-x

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Identification and characterization of the poly(ADP-ribose) glycohydrolase of Aspergillus nidulans2020

    • Author(s)
      Lisa Wise, Reini Mori, Motoyuki Shimizu, Masashi Kato
    • Organizer
      15th European Conference on Fungal Genetics
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 白色腐朽菌 Phanerochaete chrysosporium 由来の シトクロム P450 CYP505D ホモログの機能解析2020

    • Author(s)
      森玲香, ワイズ里沙, 酒井杏匠, 志水元亨, 加藤雅士
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 麹菌が生産する糖質分解酵素および麹菌そのものの新しい産業利用の可能性について2019

    • Author(s)
      志水元亨, 加藤雅士
    • Organizer
      応用糖質科学シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Aspergillus nidulans が種々の多糖応答的に生産する細胞外酵素の網羅的解析2019

    • Author(s)
      木島尚輝,酒井杏匠,糀谷紗季,山口愛彩,大堀沙貴子, 高須賀太一,堀千明,志水元亨,加藤雅士
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 麹菌摂取による宿主腸内細菌叢の改善および大腸炎の緩和2019

    • Author(s)
      兒島 孝明、志水 元亨、馬場 保徳、中野 秀雄、加藤 雅士
    • Organizer
      第71回日本生物工学会大会シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] Characterization of a new β-1,4-Mannanase belonging to a Glycoside Hydrolase Family 134 Aspergillus nidulans2019

    • Author(s)
      (15)Maho Sobue, Kiyota Sakai, Motoyuki Shimizu, Masashi Kato
    • Organizer
      30th Fungal Genetics Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 糸状菌 Aspergillus nidulans のセクレトーム解析から同定された hypothetical protein の機能解析2019

    • Author(s)
      (18)大堀沙貴子、酒井杏匠、糀谷紗季、山口愛彩、木島尚輝、松江渚、小栗莉奈、高須賀太一、堀千明、志水元亨、加藤雅士
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Aspergillus saitoi が生産する耐熱性ペクチン分解酵素の機能解析2018

    • Author(s)
      (1)鈴木健吾、酒井杏匠、上條順也、村田俊輔、和久豊、志水元亨、加藤雅士
    • Organizer
      セルラーゼ研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] Aspergillus saitoi が生産する耐熱性ペクチン分解酵素の機能解析2018

    • Author(s)
      (2)木本紗蘭、上條順也、酒井杏匠、村田俊輔、和久豊、志水元亨、加藤雅士
    • Organizer
      日本食品科学工学会第65回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 糸状菌 Aspergillus nidulans が種々の多糖応答的に生産する細胞外酵素の網羅的解析2018

    • Author(s)
      (6)山口愛彩、酒井杏匠、糀谷紗季、木島尚輝、大堀沙貴子、小栗莉奈、高須賀太一、堀千明、志水元亨、加藤雅士
    • Organizer
      日本生物工学会 2018 年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] プロテオミクスを基盤とした植物バイオマス分解に関わる糸状菌由来新規酵素の探索2018

    • Author(s)
      志水元亨
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Oxygen-radical pretreatment promotes cellulose degradation by cellulolytic enzymes2018

    • Author(s)
      Saki Kojiya, Kiyota Sakai, Junya Kamijo, Kenta Tanaka, Masahiro Maebayashi, Jun-Seok Oh, Masafumi Ito, Masaru Hori, Motoyuki shimizu, Masashi Kato
    • Organizer
      ISPlasma 2018
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 新規 GH134 ファミリーに属する糖質加水分解酵素群の機能解析2017

    • Author(s)
      新沢裕大、酒井杏匠、鈴木健吾、木本紗蘭、神藤定生、志水元亨、加藤雅士
    • Organizer
      セルラーゼ研究会 第31回 大会
    • Related Report
      2017 Research-status Report
  • [Presentation] β-マンナナーゼ Man134A 変異体の機能およびマンナンを含む食品への応用2017

    • Author(s)
      鈴木健吾、酒井杏匠、新沢裕大、嶺澤美帆、神藤定生、前林正弘、林利哉、志水元亨、加藤雅士
    • Organizer
      日本食品科学工学会 第64回 大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Aspergillus nidulansのセクレトーム解析から見出された新規ガラクタン分解酵素2017

    • Author(s)
      酒井杏匠、糀谷紗季、鈴木健吾、高須賀太一、堀千明、鈴木裕満、松江渚、志水元亨、加藤雅士
    • Organizer
      第17回 糸状菌分子生物学カンファレンス
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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