| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The aim of this study was to screen genes that can be utilized to construct a polymer production system with waste edible oil as raw material. One major component of edible oil is oleic acid. We investigated genes encoding cyclooctanone monooxygenase, an enzyme that can synthesize poly(8-octanolide) from oleic acid via cyclooctanone and 8-octanolide. Additionally, we screened for cyclohexanecarboxylic acid-degrading genes from the aromatization pathway. The encoded enzymes can synthesize 4-hydroxycyclohexanecarboxylic acid, 4-hydroxybenzoic acid, protocatechuic acid, and 2-pyrone-4,6-dicarboxylic acid from oleic acid via cyclohexanecarboxylic acid.
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