| Project/Area Number |
17K07744
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | タンパク質工学 / 一酸化窒素 / FRET / バイオセンサー / X線結晶構造解析 |
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| Outline of Final Research Achievements |
The aim of this study was to visualize the dynamics of NO in living cells using sNOOOpy (sensor for NO2/NO3 in physiology), a sensor protein system that detects changes in the concentration of nitrate and nitrite in cells, and to elucidate the physiological dynamics of NO using changes in the concentration of NO3/NO2 as an indicator. The sNOOOpy system was not sensitive enough to detect changes in NO3/NO2 concentration in living cells. In this study, we identified the complex structure of the sNOOOpy system's protein, NasS, with NO3 and NO2, and combined it with protein-level characterization to obtain information on the structural basis at the molecular level.
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| Academic Significance and Societal Importance of the Research Achievements |
細胞内の硝酸塩・亜硝酸塩の濃度変化を可視化することは、様々な生理機能に関わる一酸化窒素・NOの生理機能を解明するうえで大きなメリットがある。その方法として、sNOOOpyシステムを応用することを目指してきた。細胞内の変化を検出することはできるようになっているものの実用化までには至らなかったが、システムの基礎的な情報を収集することで今後の改良のための道筋をつけることができた。
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