| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Acyltransferases (ATs) are key determinants of building block specificity in polyketide biosynthesis. Despite the importance of protein-protein interactions between AT and carrier protein (CP) during the acyltransfer reaction, the mechanism of CP recognition by AT had been poorly understood. In this study, we chemically synthesized pantetheineamide-type cross-linking probe to capture transient AT-CP complex in its functional state, and successfully determined the crystal structure of AT-CP complex of disorazole polyketide synthase (PKS). The crystal structure of the disorazole AT-CP complex provides the first detailed insights into the protein-protein interactions between a trans-acting AT and CP in trans-AT PKS assembly lines. We also showed that the pantetheineamide-type cross-linking probe is useful for structural characterization of adenylation domain-CP complexes.
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