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Functional characterization of aroma glycosylation in plant chemical defense

Research Project

Project/Area Number 17K07750
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied biochemistry
Research InstitutionShizuoka University

Principal Investigator

Ohnishi Toshiyuki  静岡大学, 農学部, 准教授 (60542165)

Co-Investigator(Kenkyū-buntansha) 佐藤 浩平  静岡大学, 工学部, 助教 (30756705)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords配糖体 / テルペン / 香り / モノテルペン / 香気成分 / 酵素 / 植物
Outline of Final Research Achievements

aroma volatiles function as chemical defense molecules against fungi and insects.aroma volatiles are storaged as glycosides in the tuber of sweet potatoes, in the young leaves in tea, flowers in rose. Glycosylation of aroma volatiles contributes to the stable storage of aroma volatiles by reducing their volatility. Aroma glycosides are biosynthesized by UDP-sugar dependent glycosyltransferases (UGT). In this study, we enzymatically characterized that a UGT involved in aroma glycosylation in sweet potatoes,rose, tea.

Academic Significance and Societal Importance of the Research Achievements

植物における①配糖体の時間的・空間的貯蔵の仕組み,②配糖化酵素の同定,③配糖体の生理生態学的意義を明らかにすることを目的とする。本研究を通して,「香り」の受容機構の一つである“配糖化反応”を分子レベルで解明することで,植物の環境防御応答に関する新しい概念を提案することを目指した。特に,サツマイモにおける香気配糖化メカニズムを分子レベルで解明することを目的に, 香気配糖化酵素の単離・同定および酵素機能の解明において成果を得た。サツマイモは食用植物でもあり,サツマイモの食味のみならず揮発性化合物を用いた防御機構の解明の基盤となることが期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (22 results)

All 2020 2019 2018 2017

All Journal Article (12 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 11 results,  Open Access: 6 results) Presentation (9 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Book (1 results)

  • [Journal Article] Rhizotaxis Modulation in Arabidopsis Is Induced by Diffusible Compounds Produced during the Cocultivation of Arabidopsis and the Endophytic Fungus Serendipita indica2020

    • Author(s)
      Inaji Aoi、Okazawa Atsushi、Taguchi Taiki、Nakamoto Masatoshi、Katsuyama Nao、Yoshikawa Ryoka、Ohnishi Toshiyuki、Waller Frank、Ohta Daisaku
    • Journal Title

      Plant and Cell Physiology

      Volume: 61 Issue: 4 Pages: 838-850

    • DOI

      10.1093/pcp/pcaa008

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 茶の「香り」の貯蔵と生成のメカニズム2019

    • Author(s)
      大西利幸.
    • Journal Title

      香料

      Volume: 284 Pages: 29-38

    • NAID

      40022108530

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Recent advances in brassinosteroid biosynthetic pathway: insight into novel brassinosteroid shortcut pathway2018

    • Author(s)
      Ohnishi Toshiyuki
    • Journal Title

      Journal of Pesticide Science

      Volume: 43 Issue: 3 Pages: 159-167

    • DOI

      10.1584/jpestics.D18-040

    • NAID

      130007437206

    • ISSN
      1348-589X, 1349-0923
    • Year and Date
      2018-08-20
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Overexpression of geraniol synthase induces heat stress susceptibility in Nicotiana tabacum2018

    • Author(s)
      Hamachi Ashita、Nisihara Masahiro、Saito Shiori、Rim Hojun、Takahashi Hideyuki、Islam Monirul、Uemura Takuya、Ohnishi Toshiyuki、Ozawa Rika、Maffei Massimo E.、Arimura Gen-ichiro
    • Journal Title

      Planta

      Volume: 249 Issue: 1 Pages: 235-249

    • DOI

      10.1007/s00425-018-3054-z

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Identification of a Hexenal Reductase That Modulates the Composition of Green Leaf Volatiles2018

    • Author(s)
      Tanaka Toshiyuki、Ikeda Ayana、Shiojiri Kaori、Ozawa Rika、Shiki Kazumi、Nagai-Kunihiro Naoko、Fujita Kenya、Sugimoto Koichi、Yamato Katsuyuki T.、Dohra Hideo、Ohnishi Toshiyuki、Koeduka Takao、Matsui Kenji
    • Journal Title

      Plant Physiology

      Volume: 178 Issue: 2 Pages: 552-564

    • DOI

      10.1104/pp.18.00632

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Silkworms suppress the release of green leaf volatiles by mulberry leaves with an enzyme from their spinnerets2018

    • Author(s)
      Takai Hiroki、Ozawa Rika、Takabayashi Junji、Fujii Saki、Arai Kiriko、Ichiki Ryoko T.、Koeduka Takao、Dohra Hideo、Ohnishi Toshiyuki、Taketazu Sakura、Kobayashi Jun、Kainoh Yooichi、Nakamura Satoshi、Fujii Takeshi、Ishikawa Yukio、Kiuchi Takashi、Katsuma Susumu、Uefune Masayoshi、Shimada Toru、Matsui Kenji
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 11942-11942

    • DOI

      10.1038/s41598-018-30328-6

    • NAID

      120006510284

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 1-Octen-3-ol Is Formed from Its Glycoside during Processing of Soybean [Glycine max (L.) Merr.] Seeds2018

    • Author(s)
      Matsui Kenji、Takemoto Hiroyuki、Koeduka Takao、Ohnishi Toshiyuki
    • Journal Title

      Journal of Agricultural and Food Chemistry

      Volume: 66 Issue: 28 Pages: 7409-7416

    • DOI

      10.1021/acs.jafc.8b01950

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure-Based Chemical Design of Abscisic Acid Antagonists That Block PYL?PP2C Receptor Interactions2018

    • Author(s)
      Takeuchi Jun、Mimura Naoki、Okamoto Masanori、Yajima Shunsuke、Sue Masayuki、Akiyama Tomonori、Monda Keina、Iba Koh、Ohnishi Toshiyuki、Todoroki Yasushi
    • Journal Title

      ACS Chemical Biology

      Volume: 13 Issue: 5 Pages: 1313-1321

    • DOI

      10.1021/acschembio.8b00105

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] The effects of gene disruption of Kre6-like proteins on the phenotype of β-glucan-producing Aureobasidium pullulans.2018

    • Author(s)
      Uchiyama H, Iwai A, Dohra H, Ohnishi T, Kato T, Park EY
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 102 Issue: 10 Pages: 4467-4475

    • DOI

      10.1007/s00253-018-8947-z

    • NAID

      120006543632

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Conversion of carlactone to carlactonoic acid is a conserved function of MAX1 homologs in strigolactone biosynthesis.2018

    • Author(s)
      Yoneyama K, Mori N, Sato T, Yoda A, Xie X, Okamoto M, Iwanaga M, Ohnishi T, Nishiwaki H, Asami T, Yokota T, Akiyama K, Yoneyama K, Nomura T.
    • Journal Title

      New Phytol.

      Volume: 218 Issue: 4 Pages: 1522-1533

    • DOI

      10.1111/nph.15055

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] “Hidden” terpenoids in plants: their biosynthesis, localisation and ecological roles2017

    • Author(s)
      Yazaki K., Arimura G., Ohnishi T.
    • Journal Title

      Plant and Cell Physiology

      Volume: 58 Issue: 10 Pages: 1615-1621

    • DOI

      10.1093/pcp/pcx123

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Occurrence of brassinosteroids in non-flowering land plants, liverwort, moss, lycophyte and fern.2017

    • Author(s)
      Yokota T, Ohnishi T, Shibata K, Asahina M, Nomura T, Fujita T, Ishizaki K, Kohchi T.
    • Journal Title

      Phytochemistry

      Volume: 136 Pages: 46-55

    • DOI

      10.1016/j.phytochem.2016.12.020

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] 香気成分2-phenylethanol配糖化酵素の酵素学的解析2019

    • Author(s)
      中馬かれん、肥塚崇男、竹内 純、佐藤浩平、間瀬暢之、轟 泰司、鈴木秀幸、渡辺修治、大西利幸
    • Organizer
      第54回植物化学調節学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] なぜ植物は良い香りがするのだろう? ~植物の生存競争に大事な香り~2019

    • Author(s)
      大西利幸
    • Organizer
      静岡大学グリーンサイエンスカフェ
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ストリゴラクトンとアブシシン酸のシグナル伝達を制御するハイブリッド分子の創出2019

    • Author(s)
      野村恭佑、轟 泰司、大西利幸、竹内 純
    • Organizer
      第54回植物化学調節学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] トマトにおける香気単糖配糖化酵素の機能解析2019

    • Author(s)
      仲田亜里沙,切岩 祥和,竹内 純、佐藤浩平、間瀬暢之、轟 泰司、大西利幸
    • Organizer
      第54回植物化学調節学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] The biosynthetic machinery of rose aroma mono-glucosides2019

    • Author(s)
      Ohnishi T
    • Organizer
      Terpnet2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] なぜ植物は良い香りがするのだろう? ~植物の生存競争に大事な香り~2018

    • Author(s)
      大西利幸
    • Organizer
      静岡大学グリーンサイエンスカフェ
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 植物の生長や化学防御に寄与する二次代謝産物の生合成研究2017

    • Author(s)
      大西利幸
    • Organizer
      第35回日本植物細胞分子生物学会(さいたま)大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Volatile C13-norisoprenoid and monoterpene alcohols contribute sweet muscatel-like scent in Oolong tea “Oriental Beauty” manufactured by Camellia sinensis in response to attacks by insect herbivores2017

    • Author(s)
      Koujirou Totsuka, Naoya Sakai, Tsuyoshi Katsuno, Naoharu Watanabe, Toshiyuki Ohnishi
    • Organizer
      The 13th International Meeting on Biosynthesis, Function and Synthetic Biology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Study on plant terpene volatile biosynthesis2017

    • Author(s)
      Toshiyuki Ohnishi
    • Organizer
      2nd German-Japanese Research Symposiumat Technische Universitat Braunschweig
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Book] 基礎から学ぶ植物代謝生化学2018

    • Author(s)
      水谷 正治、士反 伸和、杉山 暁史
    • Total Pages
      328
    • Publisher
      羊土社
    • ISBN
      9784758120906
    • Related Report
      2018 Research-status Report

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Published: 2017-04-28   Modified: 2023-01-30  

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