Protein kinase-mediated regulation in response to N deficiency in Chlamydomonas

• Project/Area Number: 17K07753
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kindai University (2019); Kyoto University (2017-2018)
• Principal Investigator: Masataka Kajikawa 近畿大学, 生物理工学部, 講師 (40594437)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 植物分子生物学 / 代謝制御 / 脂質 / タンパク質リン酸化酵素 / 栄養欠乏応答 / 藻類 / 植物 / 栄養応答 / 窒素欠乏

Outline of Final Research Achievements

When nutrient deprived, microalgae accumulate triacylglycerol (TAG) in lipid droplets. Dual-specificity kinase, TAG-accumulation-regulator-1 (TAR1) has been reported to regulate carbon metabolism in a green alga, Chlamydomonas reinhardtii. We identified total 426 TAR1-dependent phosphoproteins by phosphoproteomic analysis (Shinkawa et al. 2019). We focused a novel protein kinase from these TAR's target proteins. It prevents the excess accumulation of triacylglycerol (TAG) in C/N-stress conditions. Phosphorylation of the continuous serine residues is TAR1-dependently induced in the C/N-stress conditions. Expression of mutated protein with Alanine substitution of these Ser residues could not complement the mutant phenotype of the kinase, suggesting the importance of phosphorylation of these serine residues for function of the kinase. We also showed this kinase protein located at plasma membrane.

Academic Significance and Societal Importance of the Research Achievements

栄養素が枯渇すると微細藻類はトリアシルグリセロール（TAG）を蓄積する。藻類の生産する油脂は化石燃料に代わる燃料源として注目されているため、その仕組みを明らかにすることは基礎科学的に興味深いだけではなく、産業応用の上でも重要である。申請者らはDYRK型タンパク質リン酸化酵素が藻類の脂質蓄積の鍵制御因子であることを初めて見出し、TAR1を起点としたタンパク質リン酸化の制御機構の詳細を解明することを本研究の目標とした。TAR1のリン酸化標的タンパク質について論文報告し、さらにTAR1の下流因子として働く新規リン酸化タンパク質を見出した。これらの成果は脂質生産の制御機構の解明に貢献すると期待される。

Research Products

• [Journal Article] Algal Autophagy Is Necessary for the Regulation of Carbon Metabolism Under Nutrient Deficiency (2020)
  • Author(s): Kajikawa, M., Fukuzawa, H.
  • Journal Title: Front. Plant Sci. Volume: 11 Pages: 35-35
  • DOI: 10.3389/fpls.2020.00036
  • Peer Reviewed / Open Access
• [Journal Article] Lipid remodeling regulator 1 (LRL1) is differently involved in the phosphorus‐depletion response from PSR1 in Chlamydomonas reinhardtii (2019)
  • Author(s): Hidayati Nur A.、Yamada‐Oshima Yui、Iwai Masako、Yamano Takashi、Kajikawa Masataka、Sakurai Nozomu、Suda Kunihiro、Sesoko Kanami、Hori Koichi、Obayashi Takeshi、Shimojima Mie、Fukuzawa Hideya、Ohta Hiroyuki
  • Journal Title: The Plant Journal Volume: 100 Issue: 3 Pages: 610-626
  • DOI: 10.1111/tpj.14473
  • Peer Reviewed / Open Access
• [Journal Article] The bZIP transcription factor regulates lipid remodeling and contributes to ER stress management in Chlamydomonas reinhardtii. (2019)
  • Author(s): Yamaoka Y, Shin S, Choi BY, Kim H, Jang S, Kajikawa M, Yamano T, Kong F, Legeret B, Fukuzawa H, Li-Beisson Y, Lee Y.
  • Journal Title: Plant Cell Volume: 31 Issue: 5 Pages: 1127-1140
  • DOI: 10.1105/tpc.18.00723
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Algal Protein Kinase, Triacylglycerol Accumulation Regulator 1, Modulates Cell Viability and Gametogenesis in Carbon/Nitrogen-Imbalanced Conditions (2019)
  • Author(s): Shinkawa H, Kajikawa M, Nomura Y, Ogura M, Sawaragi Y, Yamano T, Nakagami H, Sugiyama N, Ishihama Y, Kanesaki Y, Yoshikawa H, Fukuzawa H
  • Journal Title: Plant and Cell Physiology Volume: 60 Issue: 4 Pages: 916-930
  • DOI: 10.1093/pcp/pcz010
  • NAID: 120006823940
  • Peer Reviewed / Open Access
• [Journal Article] 実用珪藻の代謝工学による高付加価値油脂の生産 (2018)
  • Author(s): 実用珪藻の代謝工学による高付加価値油脂の生産
  • Journal Title: KAGAKU TO SEIBUTSU Volume: 56 Issue: 4 Pages: 246-247
  • DOI: 10.1271/kagakutoseibutsu.56.246
  • NAID: 130007616590
  • ISSN: 0453-073X, 1883-6852
  • Year and Date: 2018-03-20
• [Journal Article] Isolation and characterization of Chlamydomonas autophagy-related mutants in nutrient-deficient conditions. (2018)
  • Author(s): Kajikawa, M., Yamauchi, M., Shinkawa, H., Tanaka, M., Hatano, K., Nishimura, Y., Kato, M., Fukuzawa, H.
  • Journal Title: Plant Cell Physiology Volume: 60 Issue: 1 Pages: 126-138
  • DOI: 10.1093/pcp/pcy193
  • Peer Reviewed / Open Access
• [Journal Article] A rice PHD-finger protein OsTITANIA, is a growth regulator that functions through elevating expression of transporter genes for multiple metals. (2018)
  • Author(s): Tanaka, N., Uraguchi, S., Kajikawa, M., Saito, A., Ohmori, Y., Fujiwara, T.
  • Journal Title: Plant Journal Volume: 96 Issue: 5 Pages: 997-1006
  • DOI: 10.1111/tpj.14085
  • Peer Reviewed
• [Journal Article] Genomic insights into the evolution of nicotine biosynthesis pathway in tobacco (2017)
  • Author(s): Kajikawa, M., Sierro, N., Kawaguchi, H., Bakaher, N., Ivanov, N.V., Hashimoto, T., Shoji, T.
  • Journal Title: Plant Physiol. Volume: 174 Issue: 2 Pages: 999-1011
  • DOI: 10.1104/pp.17.00070
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Evolution of the nicotine biosynthesis regulon in tobacco (2017)
  • Author(s): Shoji, T., Kajikawa, M., Sierro, N., Hashimoto, T.
  • Journal Title: Plant Signal Behav. Volume: e1338225 Issue: 6 Pages: e1338225-e1338225
  • DOI: 10.1080/15592324.2017.1338225
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 藻類のタンパク質リン酸化酵素Triacylglycerol Accumulation Regulator1 はC/N ストレス条件下における細胞生存率と配偶子誘導を制御する (2020)
  • Author(s): 新川はるか，梶川昌孝，椹木裕里，山野隆志，兼崎友，吉川博文，福澤秀哉
  • Organizer: 第61日本植物生理学会年会
• [Presentation] 高CO2 かつ窒素欠乏条件でTAG を高蓄積する緑藻クラミドモナスの変異体B10 株の解析 (2020)
  • Author(s): 長房すずか，宮本あすか，新川はるか，新川友貴，山野隆志，辻敬典，梶川昌孝，福澤秀哉
  • Organizer: 第61日本植物生理学会年会
• [Presentation] C/N ストレス条件で脂質を高蓄積する緑藻変異株ccdc124 (2019)
  • Author(s): 長房すずか、宮本明日香、新川はるか、新川友貴、山野隆志、 辻敬典、梶川昌孝、福澤秀哉
  • Organizer: 第32回植物脂質シンポジウム
• [Presentation] ゼニゴケDYRK型タンパク質リン酸化酵素MpYak1は、無性芽の休眠と生殖器誘導の制御に関与する (2019)
  • Author(s): 新川はるか、梶川昌孝、西浜竜一、河内孝之、福澤秀哉
  • Organizer: 日本植物学会第83回大会
• [Presentation] オートファジーに依存した緑藻Chlamydomonas reinhardtii の生存と油脂蓄積 (2019)
  • Author(s): 梶川 昌孝、山内 万里花、新川 はるか、田中 学、幡野 恭子、西村 芳樹、加藤 美砂子、福澤 秀哉
  • Organizer: 日本藻類学会第43回大会
• [Presentation] C/N ストレス条件で葉緑素と脂質を異常蓄積する新奇緑藻変異株ccdc124 の解析 (2019)
  • Author(s): 長房 すずか、宮本 明日香、新川 はるか、新川 友貴、山野 隆志、梶川 昌孝、福澤 秀哉
  • Organizer: 日本藻類学会第43回大会
• [Presentation] 藻類での有用物質生産と脂質蓄積制御因子の同定 (2018)
  • Author(s): 梶川 昌孝
  • Organizer: 日本農芸化学会2018年度大会
  • Invited
• [Presentation] オートファジーを欠損させた緑藻における窒素欠乏応答異常 (2018)
  • Author(s): 梶川 昌孝、山内 万里香、新川 はるか、田中 学、幡野 恭子、西村 芳樹、加藤 美砂子、福澤 秀哉
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] 藻類での有用物質生産と脂質蓄積制御因子の同定 (2018)
  • Author(s): 梶川 昌孝
  • Organizer: 日本農芸化学会関西支部例会503回講演会
  • Invited
• [Presentation] オートファジーを欠損した緑藻における栄養欠乏応答異常 (2018)
  • Author(s): 梶川 昌孝
  • Organizer: 植物の栄養研究会第4回研究交流会
• [Presentation] Algal Protein Kinase TAR1 Modulates Cellular Viability and Promotes Gametogenesis in High- CO2 / Nitrogen-Deficient Conditions (2018)
  • Author(s): Haruka Shinkawa, Masataka Kajikawa, Yuri Sawaragi, Takashi Yamano, Yu Kanesaki, Hirofumi Yoshikawa, Hideya Fukuzawa
  • Organizer: 18th International Conference on the Cell and Molecular Biology of Chlamydomonas (Carnegie Institution for Science, Washington, DC)
  • Int'l Joint Research
• [Presentation] Production of ricinoleic acid-containing monoestolide triacylglycerides in an oleaginous diatom, Chaetoceros gracilis (2018)
  • Author(s): Masataka Kajikawa, Tatsuki Abe, Kentaro Ifuku, Ken-ichi Furutani, Dongyi Yan, Tomoyo Okuda, Akinori Ando, Shigenobu Kishino, Jun Ogawa, Hideya Fukuzawa
  • Organizer: 18th International Conference on the Cell and Molecular Biology of Chlamydomonas (Carnegie Institution for Science, Washington, DC)
  • Int'l Joint Research
• [Presentation] 窒素欠乏環境下で葉緑体と脂質を異常蓄積する緑藻変異株の解析 (2018)
  • Author(s): 長房すずか、宮本明日香、嶋村大亮、本庄智也、新川友貴、香西紀子、梶川昌孝、山野隆志、福澤秀哉
  • Organizer: 第36回日本植物細胞分子生物学会（広島国際会議場）
• [Presentation] 実用珪藻における有用不飽和脂肪酸リシノール酸の生産 (2018)
  • Author(s): 梶川昌孝、本庄智也、伊福健太郎、小川順、菓子野康浩、福澤秀哉
  • Organizer: 第36回日本植物細胞分子生物学会（金沢商工会議所会館）
• [Presentation] C/Nストレス条件で葉緑素と脂質を異常蓄積する新奇緑藻変異株ccdc124の解析 (2018)
  • Author(s): 長房すずか、 宮本明日香、 新川はるか, 新川友貴、山野隆志、梶川昌孝、福澤秀哉
  • Organizer: 日本藻類学会第43回大会（京都）
• [Presentation] オートファジーに依存した緑藻 Chlamydomonas reinhardtii の生存と油脂蓄積 (2018)
  • Author(s): 梶川 昌孝、山内 万里香、新川 はるか、田中 学、幡野 恭子、西村 芳樹、加藤 美砂子、福澤 秀哉
  • Organizer: 日本藻類学会第43回大会（京都）
• [Presentation] 高CO2かつ窒素欠乏環境でTAGとChlorophyllを異常蓄積する緑藻変異株の単離と解析 (2018)
  • Author(s): 宮本 明日香、新川 友貴、嶋村 大亮、本庄 智也、香西 紀子、胡 東輝、豊川 知華、新川 はるか、梶川 昌孝、山野 隆志、福澤 秀哉
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] DYRK-Type protein kinase MpYak1 is involved in rhizoid elongation and carbon allocation in response to nitrogen deficiency in Marchantia polymorpha (2018)
  • Author(s): Haruka Shinkawa, Masataka Kajikawa, Ryuichi Nishihama, Takayuki Kohchi, Hideya Fukuzawa
  • Organizer: The 16th International Student Seminar
  • Int'l Joint Research
• [Presentation] Isolation of green algal mutants accumulating high levels of TAG and chlorophyll under nitrogen deficient conditions (2018)
  • Author(s): Asuka Miyamoto, Yuki Niikawa, Daisuke Shimamura, Tomoya Honjo, Noriko Kozai, Donghui Hu, Chihana Toyokawa, Haruka Shinkawa, Masataka Kajikawa, Takashi Yamano, Hideya Fukuzawa
  • Organizer: The 16th International Student Seminar
  • Int'l Joint Research
• [Presentation] DYRK-Type Protein Kinase TAR1 Regulates Rhizoid Elongation and Carbon Allocation in Response to Nitrogen Deficiency in the Liverwort Marchantia polymorpha (2017)
  • Author(s): Haruka Shinkawa, Masataka Kajikawa, Ryuichi Nishihama, Takayuki Kohchi, Hideya Fukuzawa
  • Organizer: The 65th NIBB Conference. Renaissance of Marchantia polymorpha ‐the genome and beyond‐
  • Int'l Joint Research
• [Presentation] Isolation and characterization of a Chlamydomonas mutant with high levels of chlorophyll and TAG under nitrogen deficiency (2017)
  • Author(s): Uran Ohta, Haruka Shinkawa, Asuka Miyamoto, Masataka Kajikawa, Masafumi Hirono, Hideya Fukuzawa
  • Organizer: The 7th Asian Symposium on Plant Lipid
  • Int'l Joint Research
• [Presentation] Protein kinase TAR1 conserved in the algae and land plants regulates C/N-stress responses (2017)
  • Author(s): Masataka Kajikawa
  • Organizer: 第19回京都大学生命科学研究科シンポジウム
  • Invited