Elucidation of novel functions of marine invertebrate lectins based on their structural diversity and its application
| Project/Area Number |
17K07760
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
海野 英昭 長崎大学, 工学研究科, 助教 (10452872)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | レクチン / 糖鎖 / 海産無脊椎動物 / 立体構造 / 毒素 / タンパク質 / 蛋白質工学 / X線結晶構造解析 / 無脊椎動物 / 生理活性タンパク質 / 糖 / 分子認識 |
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| Outline of Final Research Achievements |
Lectins play important roles in cell-cell and molecule-cell interactions in organisms through specific binding to carbohydrate chains. Many lectins have been mainly isolated from land animals. However, in this study, various lectins were isolated from marine invertebrates, and their novel structures and carbohydrate recognition mechanisms were elucidated. Furthermore, various novel bioactive proteins were also discovered, including lectins, by comprehensive sequence analysis of RNA using a next-generation sequencer.
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| Academic Significance and Societal Importance of the Research Achievements |
細胞間の接着やコミュニケーションには，細胞表面糖鎖とともにレクチンが重要な役割を果たしている。海産無脊椎動物には多様な生物種が存在しており，陸上動物とは構造や特徴が大きく異なるレクチンが多く存在することが予想される。本研究はこのようなことを踏まえて，海産無脊椎動物レクチンの多様性とその活性発現機構を明らかにすることを目的として，その構造と機能を詳細に研究し，多くの新奇レクチンの立体構造や糖認識機構を明らかにした。これらの結果は，今後，海産無脊椎動物の生体防御機構に重要な知見を与えるとともに，生物の分子進化に関しても有用な基礎的情報をもたらすものと考えられる。
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Report
(4 results)
Research Products
(32 results)
