MRI-based spaciotemporal analyses of olfactory neural networks that sense odors and pheromones

• Project/Area Number: 17K07761
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kumamoto University
• Principal Investigator: YOSHINAGA Sosuke 熊本大学, 大学院生命科学研究部(薬), 講師 (00448515)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 匂い / フェロモン / 脳・神経 / MRI / 嗅覚

Outline of Final Research Achievements

Olfaction is one of the most important perceptions for animals to receive information from their environments, such as in case of species preservation and danger avoidance. The information is transmitted through odor and pheromone substances, resulting in emotional, behavioral or physiological changes. Here, we aimed to develop MRI-based fundamental technologies to detect odor-evoked brain activations and reveal the activated brain regions and their connectivities. We successfully developed a computer-based odor stimulation system to give precisely time-regulated odor stimulations, resulting in sensitivity improvement of MRI detection of odor responses.

Academic Significance and Societal Importance of the Research Achievements

本研究により、それぞれのフェロモンや匂い物質により誘起される性行動や生理的変化と、活性化される嗅神経ネットワークとの関係性を明らかにするための基盤技術の開発に成功した。この技術を利用して実験データを集積することにより、性行動に影響する物質の同定および開発、さらには、実験用マウスの作製の促進や野生ネズミの繁殖制御につながることが期待される。

Research Products

• [Journal Article] Odor stimulation by automated syringe pumps in combination with independent component analysis for BOLD-fMRI study of mouse whole brain. (2019)
  • Author(s): Hayashi, F., Takeda, M., Yuzuriha N., Yoshinaga, S., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 27 Pages: 3685-3685
  • Peer Reviewed
• [Journal Article] An awake mouse MRI method using mouse clothes for fMRI applications. (2019)
  • Author(s): Yoshinaga, S., Fujiwara, S., Kusanagi, S., Kimura, K., Araki, R., Takeda, M., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 27 Pages: 3689-3689
  • Peer Reviewed
• [Journal Article] A novel reconstruction method using regional constraints, designed for the dual-band EPI scanned with four-channel receiver coil elements. (2019)
  • Author(s): Toyoda, H., Yoshinaga, S., Takeda, M., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 27 Pages: 2440-2440
  • Peer Reviewed
• [Journal Article] BOLD-fMRI comparison of olfactory responses in the mouse whole brain, with different odors and anesthesia. (2018)
  • Author(s): Hayashi, F., Yoshinaga, S., Yuzuriha N., Takeda, M., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 26 Pages: 2309-2309
  • Peer Reviewed
• [Journal Article] A BOLD analysis of the olfactory perception system in the mouse whole brain, using independent component analysis. (2017)
  • Author(s): Funatsu, H., Hayashi, F., Yoshinaga, S., Takeda, M., Yuzuriha, N., Kusanagi, S., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 25 Pages: 5363-5363
  • Peer Reviewed
• [Journal Article] An optimized slice-GRAPPA reconstruction method to reduce leakage artifacts in small-animal multiband imaging. (2017)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., Takeda, M., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 25 Pages: 3844-3844
  • Peer Reviewed
• [Presentation] Odor stimulation by automated syringe pumps in combination with independent component analysis for BOLD-fMRI study of mouse whole brain (2019)
  • Author(s): Hayashi, F., Takeda, M., Yuzuriha, N., Yoshinaga, S., and Terasawa, H.
  • Organizer: 27th ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Int'l Joint Research
• [Presentation] An awake mouse MRI method using mouse clothes for fMRI applications (2019)
  • Author(s): Yoshinaga, S., Fujiwara, S., Kusanagi, S., Kimura, K., Araki, R., Takeda, M., and Terasawa, H.
  • Organizer: 27th ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Int'l Joint Research
• [Presentation] A novel reconstruction method using regional constraints, designed for the dual-band EPI scanned with four-channel receiver coil elements (2019)
  • Author(s): Toyoda, H., Yoshinaga, S., Takeda, M., and Terasawa, H.
  • Organizer: 27th ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Int'l Joint Research
• [Presentation] An awake mouse MRI method using mouse clothes for functional MRI (2019)
  • Author(s): Fujiwara, S., Yoshinaga, S., Kusanagi, S., Kimura, K., Araki, R., Takeda, M., and Terasawa, H.
  • Organizer: 第４７回日本磁気共鳴医学会大会
• [Presentation] Machine-controlled rigorously periodic odor stimulation and group independent component analysis for functional MRI studies of odor responses in mice (2019)
  • Author(s): Takeda, M., Hayashi, F., Yuzuriha, N., Yoshinaga, S., Terasawa, H.
  • Organizer: 第４７回日本磁気共鳴医学会大会
• [Presentation] Odor-evoked responses in mouse whole brain as detected by BOLD-fMRI analyses with periodic stimulation and independent component analysis (2019)
  • Author(s): Takeda, M., Hayashi, F., Yuzuriha, N., Yoshinaga, S., Terasawa, H.
  • Organizer: 日本生物物理学会第５７回年会
• [Presentation] 周期的刺激と独立成分解析を用いたマウス嗅覚応答の機能的MRI研究 (2019)
  • Author(s): 武田光広、林 芙優、杠 直哉、吉永壮佐、寺沢宏明
  • Organizer: 第５８回ＮＭＲ討論会
• [Presentation] BOLD-fMRI study of muscone-evoked activations in the whole brain of mice using periodic stimulation and independent component analysis (2019)
  • Author(s): Takeda, M., Hayashi, F., Yuzuriha, N., Yoshinaga, S., Terasawa, H.
  • Organizer: ISMRM日本支部第４回学術集会
• [Presentation] BOLD-fMRI comparison of olfactory responses in the mouse whole brain, with different odors and anesthesia. (2018)
  • Author(s): Hayashi, F., Yoshinaga, S., Yuzuriha, N., Takeda, M., and Terasawa, H.
  • Organizer: Joint annual meeting ISMRM-ESMRMB 2018
  • Int'l Joint Research
• [Presentation] Machine-controlled rigorously periodic odor stimulation and independent component analysis for detecting odor responses in the mouse whole brain. (2018)
  • Author(s): Hayashi, F., Yoshinaga, S., Yuzuriha, N., Takeda, M., and Terasawa, H.
  • Organizer: 第４６回日本磁気共鳴医学会大会
• [Presentation] 周期的匂い刺激と独立成分解析を利用したマウス全脳の嗅覚応答の検出 (2018)
  • Author(s): 武田光広，林芙優，杠直哉，吉永壮佐，寺沢宏明
  • Organizer: 第５７回ＮＭＲ討論会
• [Presentation] BOLD法を用いた異なる匂い刺激および麻酔下におけるマウス高次脳の嗅覚応答の比較 (2018)
  • Author(s): 林 芙優、吉永 壮佐、杠 直哉、武田 光広、寺沢 宏明
  • Organizer: ISMRM日本支部第２回学術集会
• [Presentation] A BOLD analysis of the olfactory perception system in the mouse whole brain, using independent component analysis. (2017)
  • Author(s): Funatsu, H., Hayashi, F., Yoshinaga, S., Takeda, M., Yuzuriha, N., Kusanagi, S., and Terasawa, H.
  • Organizer: 25th ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Int'l Joint Research
• [Presentation] An optimized slice-GRAPPA reconstruction method to reduce leakage artifacts in small-animal multiband imaging. (2017)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., Takeda, M., and Terasawa, H.
  • Organizer: 25th ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Int'l Joint Research
• [Presentation] Detection of the odor response in the mouse whole brain using periodic odor stimulations and independent component analysis (ICA). (2017)
  • Author(s): Hayashi, F., Funatsu, H., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., Takeda, M., and Terasawa, H.
  • Organizer: 第４５回日本磁気共鳴医学会大会
• [Presentation] Improvement of an awake mouse MRI method using mouse clothes for a cryogenic coil system. (2017)
  • Author(s): Nakata, E., Kusanagi, S., Kimura, K., Araki, R., Takeda, M., Yoshinaga, S., and Terasawa, H.
  • Organizer: 第４５回日本磁気共鳴医学会大会
• [Presentation] Detection of the odor BOLD response in the mouse whole brain, using independent component analysis (ICA). (2017)
  • Author(s): Funatsu, H., Hayashi, F., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., Takeda, M., and Terasawa, H.
  • Organizer: 第５５回日本生物物理学会年会
• [Presentation] Evaluation of anesthesia conditions for detecting odor responses in the mouse whole brain. (2017)
  • Author(s): Hayashi, F., Funatsu, H., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., Takeda, M., and Terasawa, H.
  • Organizer: 第５５回日本生物物理学会年会