Biomedicine production using earthworms-Development of earthworm transformation system-
| Project/Area Number |
17K07767
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Nagaoka National College of Technology |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | ミミズ / 形質転換法 / モデル生物 / バイオ医薬品 / 物質生産 / Eisenia / 形質転換 / 新規宿主 / 応用動物 |
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| Outline of Final Research Achievements |
The research presented here aimed to develop the earthworms Eisenia fetida Waki and E. andrei Sagami as next-generation animal protein production hosts. Although the transformation rate of our previously developed method was low, we have succeeded to improve the rate up to 48%. In addition, human erythropoietin was detected in transformed earthworm tail fragments by ELISA. These results might contribute to the development of a novel earthworms-based animal protein production system.
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| Academic Significance and Societal Importance of the Research Achievements |
現在バイオ医薬品は動物細胞を主として生産されているが，操作性やコスト面が課題となっている．そこで，次世代型の生産法として動物/植物個体を宿主として生産するヒューマノイドアニマル/プラントが研究されている．その一環としてミミズによるバイオ医薬品の生産を目指し，宿主開発を行ってきた．その結果，ヒトタンパク質の生産に微量ながら成功した事は，全く新しい宿主の誕生を意味し，開発された遺伝子組換え技術は様々な分野へ波及することが期待される．
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Report
(4 results)
Research Products
(20 results)
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[Presentation] Investigation of optimal heterologous gene transfer system of earthworm2017
Author(s)
Takeuchi, A., Yamaya, T., Machida, Y., Tsuchida, K., Isa, T., and Akazawa, S.
Organizer
2nd International Conference of “Science of Technology Innovation” 2017
Related Report
Int'l Joint Research
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