| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Previously, we found that synthesized histidine-rich peptide/proteins are efficiently internalized into mammalian cells. This finding suggests the possibility that natural histidine-rich peptide/proteins also show cellular uptake.
In this study, we focused on histidine-rich peptide (Hpn) and histidine-rich protein Ⅱ (HRP2) derived from Helicobacter pylori and Plasmodium falciparum, respectively. The Hpn has been known to form amyloid-like oligomer and thought as a pathogenicity factor for Alzheimer's disease. Interestingly, here, the Hpn showed cellular uptake and transcytosis in the human gastric epithelial tumor cells. On the other hand, the HRP2 also showed cellular uptake in various human cell lines and cytotoxic effects on human fibrosarcoma. These findings suggest a possibility that the Hpn and HRP2 play as a pathogenic factor in the human body.
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