Analysis for cell-penetrating mechanism of histidine-rich peptide/proteins
Project/Area Number |
17K07771
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Bioorganic chemistry
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Research Institution | Tottori University |
Principal Investigator |
|
Project Period (FY) |
2017-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2019)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | ヒスチジン / 細胞膜透過 / トランスサイトーシス / 細胞毒性 / 病原性因子 / ピロリ菌 / マラリア / タンパク質 / ペプチド / 蛋白質 / 細胞 |
Outline of Final Research Achievements |
Previously, we found that synthesized histidine-rich peptide/proteins are efficiently internalized into mammalian cells. This finding suggests the possibility that natural histidine-rich peptide/proteins also show cellular uptake. In this study, we focused on histidine-rich peptide (Hpn) and histidine-rich protein Ⅱ (HRP2) derived from Helicobacter pylori and Plasmodium falciparum, respectively. The Hpn has been known to form amyloid-like oligomer and thought as a pathogenicity factor for Alzheimer's disease. Interestingly, here, the Hpn showed cellular uptake and transcytosis in the human gastric epithelial tumor cells. On the other hand, the HRP2 also showed cellular uptake in various human cell lines and cytotoxic effects on human fibrosarcoma. These findings suggest a possibility that the Hpn and HRP2 play as a pathogenic factor in the human body.
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Academic Significance and Societal Importance of the Research Achievements |
本研究では、ピロリ菌HpnとマラリアHRP2が細胞内に取り込まれた後に、病原性因子として機能する可能性を明らかにした。これらのヒスチジンリッチペプチド/タンパク質を標的分子として考えることで、ピロリ菌やマラリア原虫感染症に対する新たな治療方針を開拓することができる。また、ピロリ菌HpnやマラリアHRP2以外の天然のヒスチジンリッチペプチド/タンパク質についても、同様に細胞内に取り込まれる可能性を示唆することができた。今後は、他種のヒスチジンリッチペプチド/タンパク質についてさらなる調査をすることで、新たな現象の発見につながることが期待される。
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Report
(4 results)
Research Products
(12 results)