| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
It was found that a protein having a cell-damaging activity exists in the culture medium of Bacillus thuringiensis (BT). After protein purification, the 297_1_00946 gene present on the A297 strain genome was considered to be a target protein candidate. Moreover, since the condition showing no activity was found depending on the culture conditions, a comparative analysis of gene expression levels under both culture conditions was performed. As a result, it was found that there are differences in the expression levels of genes related to the production of two secondary metabolites and one type of enterotoxin, and further analysis is being conducted. In addition, analysis of cell-damaging activity using a fluorescent probe strongly suggested that it acts on the cell membrane of cultured cells.
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