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Estimation of absorption dynamics of health concerned chemicals in edible oil

Research Project

Project/Area Number 17K07811
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Food science
Research InstitutionOsaka Research Institute of Industrial Science and Technology

Principal Investigator

Watanabe Yomi  地方独立行政法人大阪産業技術研究所, 森之宮センター, 研究室長 (60416310)

Co-Investigator(Kenkyū-buntansha) 室田 佳恵子  島根大学, 学術研究院農生命科学系, 教授 (40294681)
佐藤 博文  地方独立行政法人大阪産業技術研究所, 森之宮センター, 主任研究員 (70443546)
湯浅 明子 (小島明子)  大阪公立大学, 大学院生活科学研究科, 准教授 (90295709)
吉井 未貴  地方独立行政法人大阪産業技術研究所, 森之宮センター, 研究員 (90826158)
松尾 道憲  京都女子大学, 家政学部, 教授 (00335308)
Project Period (FY) 2017-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsモノクロロプロパンジオール / 油脂 / 細胞毒性 / 体内動態 / 吸収 / グリシドール / モノクロロプロパノンジオール / クロロプロパノール / 吸収性 / MCPD / 毒性 / LCMS / 吸収性試験
Outline of Final Research Achievements

This study attempted to estimate the disposition of MCPD, glycidol, and their fatty acid esters, which are detected in trace amounts in edible oils, processed oils and fats etc. Glycidol (fatty acyl ester) is rapidly converted to glycerol (MAG) under acidic conditions by gastric acid, but remained unchanged in the neutral conditions. In other words, the detoxification ability of gastric acid is exerted on an empty stomach, but is not expected on a full stomach unfortunately. In the intestinal tract, MCPD esters were estimated to undergo enzymatic degradation and absorption, with free MCPD entering the blood and MCPD diesters entering the lymphatic fluid by the same pathway as TAG. 2-MCPD was the least cytotoxic, followed by 3-MCPD < DCP < glycidol < acrylamide. Cytotoxicity of 2- and 3- MCPD fatty acid monoesters were not detected in vitro.

Academic Significance and Societal Importance of the Research Achievements

本研究では、2-及び3-MCPD脂肪酸エステルが腸管で分解吸収されると推定され、2-MCPDの培養細胞に対する毒性は3-MCPDより1/2~1/10程度低いことを確認した。食用油脂中に検出される2-MCPDエステル量は3-MCPDエステルの1/2~2/3程度なので、食品中の2-MCPDの健康に対するリスクは3-MCPDより低いと結論される。一方、MCPDより強い毒性を示すグリシドールは、胃酸酸性下でエポキシ基が開環して解毒されうるが、pH中性の満腹時に解毒能は期待できないことも確認した。これらの情報は、食品中のリスク物質の濃度管理を検討する際に科学的根拠を提供する点で意義が大きい。

Report

(7 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (49 results)

All 2022 2021 2020 2019 2018 2017

All Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 2 results) Presentation (37 results) (of which Int'l Joint Research: 20 results,  Invited: 9 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Advances in characterization of triacylglycerols: Expansion of materials used in Joint <scp>JOCS</scp> / <scp>AOCS</scp> Official Method Ch 3a‐192022

    • Author(s)
      Mochizuki Mamiko、Watanabe Yomi、Taha Ameer Y.、Masuyama Araki
    • Journal Title

      Journal of the American Oil Chemists' Society

      Volume: 99 Issue: 6 Pages: 535-540

    • DOI

      10.1002/aocs.12592

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Improvements in extraction yield by solid phase lipid extraction from liquid infant formula and human milk, and the fatty acid distribution in milk TAG analyzed by joint JOCS/AOCS official method Ch 3a-192022

    • Author(s)
      Watanabe Yomi、Hirokawa Motoko、Furukawa Arisa、Kawasaki Lisa、Takumi Hiroko、Masuyama Araki
    • Journal Title

      Frontiers in Nutrition

      Volume: 9

    • DOI

      10.3389/fnut.2022.970837

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Deracemization of 1-phenylethanols in a one-pot process combining Mn-driven oxidation with enzymatic reduction utilizing a compartmentalization technique2022

    • Author(s)
      Sato Hirofumi、Yamada Rei、Watanabe Yomi、Kiryu Takaaki、Kawano Shintaro、Shizuma Motohiro、Kawasaki Hideya
    • Journal Title

      RSC Advances

      Volume: 12 Issue: 17 Pages: 10619-10624

    • DOI

      10.1039/d2ra01326f

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 脂質開発に役立つ2位脂肪酸組成分析法2022

    • Author(s)
      渡辺嘉
    • Journal Title

      Food Style21

      Volume: 26 Pages: 68-71

    • Related Report
      2022 Annual Research Report
  • [Journal Article] 油脂加工に有用な脂質分解酵素反応系と分析法の開発2022

    • Author(s)
      渡辺嘉
    • Journal Title

      食品の試験と研究

      Volume: 57 Pages: 115-121

    • Related Report
      2022 Annual Research Report
  • [Journal Article] Fatty acid composition and distribution in triacylglycerols of cultured fishes in Japan2021

    • Author(s)
      Mamiko Mochizuki, Hajime Matsuyama, Kunihiro Suzuki, Yomi Watanabe
    • Journal Title

      Biocatalysis and Agricultural Biotechnology

      Volume: 31 Pages: 101687-101687

    • DOI

      10.1016/j.bcab.2020.101687

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Analysis of fatty acid distribution in lipid extracts of infant formulas2020

    • Author(s)
      Yomi Watanabe, Lisa Kawasaki, Araki Masuyama
    • Journal Title

      Milk Science

      Volume: 69 Pages: 63-70

    • NAID

      130007886352

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Monoacylglycerol of 7,10-Dihydroxy-8(E)-octadecenoic Acid Enhances Antibacterial Activities against Food-Borne Bacteria.2019

    • Author(s)
      Chen KY, Kim IH, Hou CT, Watanabe Y, Kim HR
    • Journal Title

      Journal of agricultural and food chemistry

      Volume: 67 Issue: 29 Pages: 8191-8196

    • DOI

      10.1021/acs.jafc.9b03063

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] モノクロロプロパンジオール吸収動態に関する研究2019

    • Author(s)
      渡辺嘉、佐藤博文
    • Journal Title

      オレオサイエンス

      Volume: 19 Pages: 247-248

    • Related Report
      2019 Research-status Report
  • [Journal Article] Roe-derived phospholipid administration enhances lymphatic docosahexaenoic acid-containing phospholipid absorption in unanesthetized rats2017

    • Author(s)
      Murota Kaeko、Takagi Mika、Watanabe Yomi、Tokumura Akira、Ohkubo Takeshi
    • Journal Title

      Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA)

      Volume: - Pages: 40-48

    • DOI

      10.1016/j.plefa.2017.06.011

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] Extending the lifetime of frying oil by cleaning the fryer2022

    • Author(s)
      Noboru Ohyagi, Takayuki Morita, Ryoko Konishi, Makiko Mochizuki, Yomi Watanabe
    • Organizer
      The 2nd World Congress on Oleo Science
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 油脂加工に有用な脂質分解酵素反応系と2位脂肪酸組成分析法の開発2022

    • Author(s)
      渡辺 嘉
    • Organizer
      日本食品科学工学会第69回大会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Improved lipid extraction method for the analysis of fatty acid distribution in TAG of infant formula2021

    • Author(s)
      Y Watanabe, H Sato, M Muraoka.
    • Organizer
      2021 AOCS Annual Meeting and Expo.
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] 固相法による乳児用調製乳からの脂質抽出2021

    • Author(s)
      川崎莉沙 渡辺嘉 佐藤博文 村岡雅弘 益山新樹
    • Organizer
      日本油化学会第60回年会
    • Related Report
      2021 Research-status Report
  • [Presentation] FA Distribution in Infant Formula Lipids Analysed by Joint JOCS/AOCS Official Method Ch 3a-20192020

    • Author(s)
      Yomi Watanabe, Araki Masuyama
    • Organizer
      Virtual 2020 AOCS Annual Meeting & Expo
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Simplification of Lipid Extraction Method and Applicability of New Calb in Fatty Acid Distribution Analysis2020

    • Author(s)
      Mamiko Mochizuki, Yomi Watanabe, Ameer Taha
    • Organizer
      Virtual 2020 AOCS Annual Meeting & Expo
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] Improvement in enzymatic enrichment of DHA in algal lipids by thermostable lipase preparation2019

    • Author(s)
      Y. Watanabe, T. Aki, A. Masuyama
    • Organizer
      The 2019 AOCS Annual Meeting & Expo
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] FA analysis in sn-2 position of different commercial olive oils by JOCS Standard Method 2.4.5-2016/AOCS Ch3a-192019

    • Author(s)
      Y. Watanabe, A. Masuyama
    • Organizer
      The 15th International Symposium on Biocatalysis and Agricultural Biotechnology
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] トリアシルグリセリン中の脂肪酸分布分析のための新酵素法2019

    • Author(s)
      渡辺 嘉
    • Organizer
      日本油化学会第58回年会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Enzymatic concentration of w-3 fatty acids in algal oil and the analysis of positional distribution.2019

    • Author(s)
      Y. Watanabe, T. Aki, A. Masuyama
    • Organizer
      17th Euro Fed Lipid Congress And Expo
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] サケ科魚類の脂質を用いた脂肪酸分布分析における酵素及び固相抽出法の検討2019

    • Author(s)
      望月万美子、渡辺 嘉
    • Organizer
      水産利用関係研究開発推進会議
    • Related Report
      2019 Research-status Report
  • [Presentation] 大型ニジマスの短期絶食における脂質、特に脂肪酸分布への影響2018

    • Author(s)
      望月万美子、渡辺 嘉
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2018 Research-status Report 2017 Research-status Report
  • [Presentation] Toxicological evaluation of 2-MCPD and estimation of intestinal absorption of its fatty acyl esters2018

    • Author(s)
      Yomi Watanabe, Naoki Kaze, Kaeko Murota, Hirofumi Sato, Yuri Osafune, Araki Masuyama
    • Organizer
      第15回日中韓酵素工学会議
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Our challenges to develop bioprocess for lignin paint2018

    • Author(s)
      Yomi Watanabe, Daisuke Koma, Masao Yamazaki
    • Organizer
      2018 AOCS Annual Meeting & Expo
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Efficiency improvement in the enzymatic fractionation of PUFA2018

    • Author(s)
      Yomi Watanabe,Ryosuke Hoshina, Kazumi Katagiri, Hideaki Kobayashi
    • Organizer
      2018 AOCS Annual Meeting & Expo
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Toxicity evaluation of 2-MCPD and estimation of intestinal absorption of the monoesters2018

    • Author(s)
      Yomi Watanabe, Naoki Kaze, Kaeko Murota, Hirofumi Sato, Yuri Osafune, Araki Masuyama
    • Organizer
      2018 AOCS Annual Meeting & Expo
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Enzymatic preparation of food and medical materials from fish oil2018

    • Author(s)
      Kazumi Katagiri, Yomi Watanabe, Ryosuke Hoshina, Hideaki Kobayashi
    • Organizer
      2018 AOCS Annual Meeting & Expo
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 静岡県産養殖魚類の脂肪酸分布について2018

    • Author(s)
      望月万美子、松山創、鈴木邦弘、渡辺嘉
    • Organizer
      水産利用関係研究開発推進会議利用加工技術部会
    • Related Report
      2018 Research-status Report
  • [Presentation] 高度不飽和脂肪酸分画における新酵素剤の有効性2018

    • Author(s)
      片山のはら・渡辺嘉・益山新樹
    • Organizer
      第57回日本油化学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 2-モノクロロプロパンジオールのHep G2細胞に対する毒性評価2018

    • Author(s)
      長船有里, 渡辺嘉, 佐藤博文, 小林正治, 益山新樹
    • Organizer
      第2回オレオマテリアル学術交流会
    • Related Report
      2018 Research-status Report
  • [Presentation] Hep G2細胞に対するクロロプロパノール類の毒性評価2018

    • Author(s)
      長船有里, 渡辺嘉, 佐藤博文, 小林正治, 益山新樹
    • Organizer
      第57回日本油化学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] リパーゼ処理によるイワシ油からのEPAエチルエステルおよびsn-2位結合DHA構造脂質調製法の検討2018

    • Author(s)
      片桐一美・渡辺嘉・保科亮祐・小林英明
    • Organizer
      第57回日本油化学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Cytotoxicity evaluation of 2-monochloropropanediol2018

    • Author(s)
      Yomi Watanabe, Yuri Osafune, Hirofumi Sato, Taro Kiso, Araki Masuyama
    • Organizer
      "The 14th International Symposium on Biocatalysis and Agricultural Biotechnology "
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] FA analysis in sn-2 position of different commercial olive oils by JOS Standard method 2.4.5-20162017

    • Author(s)
      Ryota Aoyama, Yomi Watanabe, Araki Masuyama
    • Organizer
      The Asian Conference on Oleo Science 2017 and The 56th Annual Meeting of the Japan Oil Chemists' Society
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Continuous Production of Biodiesel Fuel using Bioreactor2017

    • Author(s)
      Yuri Osafune, Yomi Watanabe,Moriatsu Ueda, Shoji Kobayashi, and Araki Masuyama
    • Organizer
      The Asian Conference on Oleo Science 2017 and The 56th Annual Meeting of the Japan Oil Chemists' Society
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Enzymatic fractionation of omega-3 fatty acids and analysis of positional distribution in oils2017

    • Author(s)
      Yomi Watanabe
    • Organizer
      The Asian Conference on Oleo Science 2017 and The 56th Annual Meeting of the Japan Oil Chemists' Society
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Lowering the Temperature Improves Enzymatic Prefractionation of EPA and DHA.2017

    • Author(s)
      Ryosuke Hoshina, Yomi Watanabe, Kazumi Katagiri, Hideaki Kobayashi
    • Organizer
      2017 AOCS Annual Meeting and Industrial Shocases
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Concentration of PUFA in Auranthiochytrium sp. single cell oil by liquid lipase preparation2017

    • Author(s)
      Yomi Watanabe, Tsunehiro Aki, Araki Masuyama
    • Organizer
      2017 AOCS Annual Meeting and Industrial Shocases
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] MCPDエステルの代謝初期動態のin vitro推定2017

    • Author(s)
      渡辺嘉
    • Organizer
      第25回加工油脂栄養研究会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Positional distribution of fatty acid in triacylglycerols of large rainbow trout cultivated in Shizuoka prefecture2017

    • Author(s)
      Mamiko Mochizuki, Yomi Watanabe
    • Organizer
      日本水産学会国際シンポジウム
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 酵素による油脂加工法・分析法の開発2017

    • Author(s)
      渡辺嘉
    • Organizer
      第一回オレオマテリアル学術交流会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Bioprocess to development of Urushi-like biomass paint2017

    • Author(s)
      Yomi Watanabe
    • Organizer
      19th Japanese-German Workshop on Enzyme Technology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 膵酵素による食事性リン脂質の消化性に及ぼす構成脂肪酸の影響2017

    • Author(s)
      平野明伽音、高木美佳、渡辺嘉、大久保剛、室田佳恵子
    • Organizer
      第22回日本フードファクター学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 大型ニジマスの絶食時における脂質変化について2017

    • Author(s)
      望月万美子、渡辺 嘉
    • Organizer
      平成29年度水産利用関係研究開発推進会議「利用加工技術部会」「資源利用研究会」
    • Related Report
      2017 Research-status Report
  • [Presentation] TokyoGreenを基盤とした効率的なリパーゼ検出のための蛍光プローブの設計と合成2017

    • Author(s)
      三輪 真之,渡辺 嘉, 靜間 基博,川崎 英也,佐藤 博文
    • Organizer
      オレオマテリアル部会関西地区2017年ウィークエンドセミナー
    • Related Report
      2017 Research-status Report
  • [Presentation] 基準油脂分析試験法2.4.5-2016によるオリーブ油と高融点油脂の2位脂肪酸組成分析2017

    • Author(s)
      青山凌大、渡辺嘉、益山新樹
    • Organizer
      オレオマテリアル部会関西地区2017年ウィークエンドセミナー
    • Related Report
      2017 Research-status Report
  • [Presentation] バイオリアクターを用いたバイオディーゼルの連続製造2017

    • Author(s)
      長船有里、渡辺嘉、益山新樹
    • Organizer
      オレオマテリアル部会関西地区2017年ウィークエンドセミナー
    • Related Report
      2017 Research-status Report
  • [Book] Food Lipids, Chapter 62017

    • Author(s)
      Yomi Watanabe and Kazuaki Yoshinaga, Casimir C. Akoh ed.
    • Total Pages
      1047
    • Publisher
      CRC Press
    • ISBN
      9781498744874
    • Related Report
      2017 Research-status Report
  • [Patent(Industrial Property Rights)] チロソール生産微生物2017

    • Inventor(s)
      駒大輔、西村(渡辺)嘉、山崎昌男
    • Industrial Property Rights Holder
      大阪産業技術研究所、オイケム合同会社
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-068262
    • Filing Date
      2017
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2024-01-30  

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