Development of quantification method using quantitative NMR for the food component

• Project/Area Number: 17K07822
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Nihon University
• Principal Investigator: OHTSUKI Takashi 日本大学, 生物資源科学部, 講師 (30401011)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 定量NMR / アクテオシド / ペダリイン / 1H-qNMR / 絶対定量 / 食品成分 / AQARI / 食品分析

Outline of Final Research Achievements

The quality assurance of quantitative values for evaluating food quality and safety has become an important issue in Japan and abroad, and the development of reliable analytical methods for various food-related components is desired. This study investigated establishing a method for analyzing food-related components by 1H-qNMR, which does not require a calibrant for the analyte.
As a result, we established an analytical method that can accurately measure the content of acteosides in foods and food materials using 1H-qNMR. An analytical method using the molar absorption coefficient ratio based on 1H-qNMR was also investigated for acteoside and pedaline. It was found that this method can quantify these two components in young sesame leaf powder and processed foods with the same accuracy as the conventional method.

Academic Significance and Societal Importance of the Research Achievements

本研究の遂行により、1H-qNMRを用いた分析法は食品のような複雑な混合物中の特定成分について、その定量用標品を使わず精確に定量できるとともに前処理の簡便化、分析時間の短縮につながることが実証され、食品素材や食薬資源の品質、同等性の評価等にも適用可能と予想された。また、モル吸光係数比を用いた分析法は、1H-qNMRと同程度に正確な定量を汎用的なHPLCにより可能とし、定量用標品がない天然成分などの含量評価法としても利用が可能である。これは、新たな食品素材の開発、未利用または低利用な生物資源の有用性の解明、新しい機能性成分や創薬原型分子の発見に大きく貢献できるものと期待される。

Research Products

• [Journal Article] Development of an HPLC method with relative molar sensitivity based on 1H-qNMR to determine acteoside and pedaliin in dried sesame leaf powders and processed foods (2020)
  • Author(s): Ohtsuki Takashi、Matsuoka Kiyoaki、Fuji Yushiro、Nishizaki Yuzo、Masumoto Naoko、Sugimoto Naoki、Sato Kyoko、Matsufuji Hiroshi
  • Journal Title: PLOS ONE Volume: 15 Issue: 12 Pages: e0243175-e0243175
  • DOI: 10.1371/journal.pone.0243175
  • Peer Reviewed / Open Access
• [Presentation] 1H-qNMR に基づく相対モル感度を用いたゴマ若葉抽出物等に含まれるアクテオシドの定量について (2019)
  • Author(s): 松岡聖朗，大槻崇，藤佑志郎，松下明里，松田美優，西崎雄三，増本直子，杉本直樹，佐藤恭子，松藤寛
  • Organizer: 日本食品化学学会第25回総会・学術大会
• [Presentation] フラバノン配糖体定量分析への1H-qNMRに基づく相対モル感度法の応用 (2019)
  • Author(s): 松岡聖朗，大槻崇，石附京子，藤佑志郎，西崎雄三，増本直子，杉本直樹，佐藤恭子，松藤寛
  • Organizer: 日本食品科学工学会第66回大会
• [Presentation] 1H-qNMRに基づく相対モル感度を用いたラカンカ抽出物中のモグロシドV分析法の確立 (2019)
  • Author(s): 大槻崇，松田美優，松下明里，小島豪，松岡聖朗，西﨑雄三，増本直子，山﨑太一，黒江美穂，沼田雅彦，井原俊英，杉本直樹，佐藤恭子，松藤寛
  • Organizer: 日本薬学会第139年会