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Aggregation control and stabilization of hydrocolloid foods using edible polyelectrolyte complexes

Research Project

Project/Area Number 17K07824
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Food science
Research InstitutionTokyo City University

Principal Investigator

Kuroiwa Takashi  東京都市大学, 工学部, 教授 (60425551)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords食品工学 / 食品コロイド / 乳化・分散 / 高分子電解質 / 安定性 / 複合化 / カゼインタンパク / キトサン
Outline of Final Research Achievements

Many polysaccharides and proteins constituting various foods are polyelectrolytes, that generate polycations, polyanions and polymeric zwitterions in aqueous solutions. In this study, development and functionalization of food colloidal dispersion systems using complexation of edible polyelectrolytes were investigated. The main findings are as follows.
(1) A simple method for preparing submicrometer-sized fine particle dispersion via self-complexation of chitosan and fatty acids was developed. Long-term stability and high encapsulation ability of chitosan-fatty acid complex particles could be demonstrated.
(2) Remarkable stabilization of palm oil-in-water emulsions was achieved using a polyelectrolyte complex layer consisting of casein proteins and chitosan.

Academic Significance and Societal Importance of the Research Achievements

乳製品、果汁飲料、ドレッシング類のような乳化・混濁食品の多くは、多糖類やタンパク質をはじめとする可食性高分子電解質が食品の安定化に寄与しているコロイド分散系である。本研究では、食品由来の高分子電解質が他の食品成分と複合化して得られる高分子電解質複合体の形成過程を積極的に制御しこれを活用することで、コロイド食品の安定化および機能化を達成できることを示した。この成果の学術的意義は、高分子電解質と食品成分の相互作用に注目してコロイド食品の分散・凝集挙動を明らかにした点にある。実際の食品の加工および流通・保存時における安定性向上につながる点で、応用面でも高い波及効果が期待できる有意義な成果が得られた。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (39 results)

All 2020 2019 2018 2017 Other

All Journal Article (7 results) (of which Peer Reviewed: 6 results,  Open Access: 3 results) Presentation (28 results) (of which Int'l Joint Research: 6 results,  Invited: 4 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Lipid vesicle preparation using W/O/W emulsions via solvent evaporation: the effect of emulsifiers on the entrapment yield of hydrophilic materials2019

    • Author(s)
      Emmanuel Chekwube Ossai, Takashi Kuroiwa, Kaname Horikoshi, Yuya Otsuka, Junki Terasawa, Akihiko Kanazawa, Seigo Sato, Sosaku Ichikawa
    • Journal Title

      Journal of the American Oil Chemists' Society

      Volume: 96 Issue: 12 Pages: 1405-1416

    • DOI

      10.1002/aocs.12275

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of dyhydrocholic acid conjugated with a hydrocarbon on a lipid bilayer composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine2019

    • Author(s)
      Keita Hayashi, Kazutoshi Morimoto, Toshiyuki Kamei, Eiko Mieda, Sosaku Ichikawa, Takashi Kuroiwa, Sakiko Fujita, Hidemi Nakamura, Hiroshi Umakoshi
    • Journal Title

      Colloids and Surfaces B: Biointerfaces

      Volume: 181 Pages: 58-65

    • DOI

      10.1016/j.colsurfb.2019.05.009

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of Lipid Vesicles Using Double Emulsions2019

    • Author(s)
      3. 黒岩 崇, 市川創作
    • Journal Title

      Oleoscience

      Volume: 19 Issue: 5 Pages: 197-201

    • DOI

      10.5650/oleoscience.19.197

    • NAID

      130007711525

    • ISSN
      1345-8949, 2187-3461
    • Related Report
      2019 Annual Research Report
    • Open Access
  • [Journal Article] Formulation and Application of Edible Nano/Micro Dispersion Systems2017

    • Author(s)
      黒岩崇
    • Journal Title

      Japan Journal of Food Engineering

      Volume: 18 Issue: 4 Pages: 161-167

    • DOI

      10.11301/jsfe.17506

    • NAID

      130006292654

    • ISSN
      1345-7942, 1884-5924
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Freeze-dryable lipid vesicles with size tunability and high encapsulation efficiency prepared by the multiple emulsification-solvent evaporation method2017

    • Author(s)
      Akihiko Suzuki, Takashi Kuroiwa, Kaname Horikoshi, Akihiko Kanazawa, Sosaku Ichikawa
    • Journal Title

      Colloids Surf. B: Biointerfaces

      Volume: 159 Pages: 412-418

    • DOI

      10.1016/j.colsurfb.2017.07.092

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hydration-aggregation pretreatment for drastically improving esterification activity of commercial lipases in non-aqueous media2017

    • Author(s)
      Maho Katayama, Takashi Kuroiwa, Kenya Suzuno, Ayumi Igusa, Toru Matsui, Akihiko Kanazawa
    • Journal Title

      Enzyme Microb. Technol.

      Volume: 105 Pages: 30-37

    • DOI

      10.1016/j.enzmictec.2017.06.007

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Preparation Characteristics of Water-in-oil Emulsion Using Olive Oil as a Continuous Phase in Microchannel Emulsification2017

    • Author(s)
      Miki Ito, Midori Uehara, Ryota Wakui, Makoto Shiota, Takashi Kuroiwa
    • Journal Title

      Japan Journal of Food Engineering

      Volume: 18 Issue: 2 Pages: 103-111

    • DOI

      10.11301/jsfe.17489

    • NAID

      130005709366

    • ISSN
      1345-7942, 1884-5924
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 生体分子間相互作用を利用した食品用分散系の作製と機能化2020

    • Author(s)
      黒岩崇
    • Organizer
      高分子と水・分離に関する研究会・2019年度界面動電現象研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Improved stability of solid-fat microspheres by electrostatic biopolymer deposition technique2019

    • Author(s)
      Yaeko Okuyama, Takashi Kuroiwa, Akihiko Kanazawa
    • Organizer
      18th Asian Pacific Confederation of Chemical Engineering Congress
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of microbioreaction systems in lipid vesicles prepared by the multiple emulsification-solvent evaporation method2019

    • Author(s)
      Hirofumi Konno, Sosaku Ichikawa, Akihiko Kanazawa, Takashi Kuroiwa
    • Organizer
      18th Asian Pacific Confederation of Chemical Engineering Congress
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] One-step encapsulation of water-insoluble bioactive compounds into chitosan-oleic acid complex particles and their characteristics as food dispersions2019

    • Author(s)
      Hiroki Shino, Tatsuhito Suzuki, Azumi Iida, Akihiko Kanazawa, Takashi Kuroiwa
    • Organizer
      18th Asian Pacific Confederation of Chemical Engineering Congress
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 酵素封入ダブルエマルションを利用した脂質ベシクル内酵素反応系の構築と特性評価2019

    • Author(s)
      今野裕史, 黒岩崇, 市川創作, 金澤昭彦
    • Organizer
      日本食品工学会第20回(2019年度)年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 脂溶性栄養機能成分を担持したキトサン-オレイン酸複合微粒子の安定性評価2019

    • Author(s)
      士野紘輝, 鈴木達仁, 飯田あづみ, 金澤昭彦, 黒岩崇
    • Organizer
      日本食品工学会第20回(2019年度)年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 可食性高分子複合層の形成が固体油脂マイクロスフィアの安定性に及ぼす影響2019

    • Author(s)
      奥山椰英子, 黒岩崇, 金澤昭彦
    • Organizer
      日本食品工学会第20回(2019年度)年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 多相エマルション法の液中乾燥工程における脂質ベシクル形成機構の解析2019

    • Author(s)
      羽田野哲史, 黒岩崇, 市川創作
    • Organizer
      日本膜学会第41年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 酵素と膜脂質を含有するダブルエマルションを利用したベシクル内酵素反応系の構築2019

    • Author(s)
      今野裕史, 市川創作, 金澤昭彦, 黒岩崇
    • Organizer
      日本膜学会第41年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 消化吸収動態を考慮した栄養機能成分キャリアの開発と食品デザインへの展開2019

    • Author(s)
      黒岩崇
    • Organizer
      バイオ計測技術コンソーシアム第125回定例会講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 生体由来成分を利用したマイクロ・ナノ分散系の作製および機能開発2019

    • Author(s)
      黒岩崇
    • Organizer
      FOOMA JAPAN 日本食品工学会フォーラム2019
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Preparation of monodisperse solid-fat microspheres and their stabilization by surface modification using chitosan2018

    • Author(s)
      Yaeko Okuyama, Takashi Kuroiwa, Akihiko Kanazawa
    • Organizer
      The 14th International Chitin and Chitosan Conference & 12th Asia-Pacific Chitin and Chitosan Symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Preparation and characterization of chitosan-fatty acid complex particles as a novel carrier material for hydrophobic bioactive molecules2018

    • Author(s)
      Takashi Kuroiwa, Yuto Kawauchi, Ryutaro Moriyoshi, Tatsuhito Suzuki, Akihiko Kanazawa
    • Organizer
      The 14th International Chitin and Chitosan Conference & 12th Asia-Pacific Chitin and Chitosan Symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] キトサン-オレイン酸複合微粒子の粒子特性と分散安定性に対する操作因子の影響2018

    • Author(s)
      鈴木達仁, 黒岩崇, 市川創作, 金澤昭彦
    • Organizer
      化学工学会第50回秋季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 多相乳化-液中乾燥プロセスによる脂質ベシクルへの水溶性酵素の内包化2018

    • Author(s)
      今野裕史, 黒岩崇, 市川創作, 金澤昭彦
    • Organizer
      化学工学会第50回秋季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] タンパク質-多糖複合層の形成による固体油脂マイクロスフィアの安定化2018

    • Author(s)
      奥山椰英子, 黒岩崇, 金澤昭彦
    • Organizer
      化学工学会第50回秋季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] キトサン-脂肪酸複合微粒子における疎水性領域の形成と脂溶性機能成分の担持効率2018

    • Author(s)
      黒岩崇, 樋口佳樹, 河内勇人, 市川創作, 金澤昭彦
    • Organizer
      化学工学会第50回秋季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] キトサン-脂肪酸複合微粒子分散液の凍結乾燥と再分散処理における粒子特性への影響因子2018

    • Author(s)
      鈴木達仁, 黒岩崇, 金澤昭彦
    • Organizer
      日本食品工学会第19回(2018年度)年次大会
    • Related Report
      2018 Research-status Report
  • [Presentation] キトサン被覆した均一径固体油脂マイクロスフィアの作製と安定性評価2018

    • Author(s)
      奥山椰英子, 黒岩崇, 金澤昭彦
    • Organizer
      日本食品工学会第19回(2018年度)年次大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ダブルエマルションの液中乾燥処理による脂質ベシクルへの酵素の内包化と特性評価2018

    • Author(s)
      今野裕史, 黒岩崇, 市川創作, 金澤昭彦
    • Organizer
      日本食品工学会第19回(2018年度)年次大会
    • Related Report
      2018 Research-status Report
  • [Presentation] カプサイシンを担持したキトサン-オレイン酸複合微粒子の形成挙動と分散安定性の評価2018

    • Author(s)
      黒岩崇, 樋口佳樹, 金澤昭彦
    • Organizer
      日本食品工学会第19回(2018年度)年次大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 多相エマルション法を利用した酵素内包ジャイアントベシクルの作製と特性評価2018

    • Author(s)
      今野裕史, 市川創作, 金澤昭彦, 黒岩 崇
    • Organizer
      日本膜学会第40年会
    • Related Report
      2018 Research-status Report
  • [Presentation] High-speed analysis of droplet formation in microchannel emulsification using vegetable oils2017

    • Author(s)
      Miki Ito, Takashi Kuroiwa, Akihiko Kanazawa
    • Organizer
      The 17th Congress of Asian Pacific Confederation of Chemical Engineering
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] キトサン-脂肪酸複合微粒子への機能性成分の担持と濃縮・凍結乾燥後の分散安定性2017

    • Author(s)
      黒岩 崇, 鈴木達仁, 河内勇人, 森吉竜太郎, 金澤昭彦
    • Organizer
      化学工学会第49回秋季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 植物油を利用したマイクロチャネル乳化による単分散エマルション形成に及ぼす要因解析2017

    • Author(s)
      伊藤美希, 黒岩 崇, 金澤昭彦
    • Organizer
      化学工学会第49回秋季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] マイクロチャネルを利用した植物油脂含有単分散エマルションの作製2017

    • Author(s)
      伊藤美希, 黒岩 崇, 金澤昭彦
    • Organizer
      日本食品工学会第18回(2017年度)年次大会
    • Related Report
      2017 Research-status Report
  • [Presentation] キトサン-オレイン酸複合サブミクロン粒子の濃縮および粉末化における分散安定性2017

    • Author(s)
      鈴木達仁, 黒岩 崇, 河内勇人, 金澤昭彦
    • Organizer
      日本食品工学会第18回(2017年度)年次大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 食用ナノ/マイクロ分散系の作製と利用に関する研究2017

    • Author(s)
      黒岩崇
    • Organizer
      日本食品工学会第18回(2017年度)年次大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Book] 生物化学工学の基礎2018

    • Author(s)
      松井徹, 上田誠, 黒岩崇, 武田穣, 徳田宏晴
    • Total Pages
      232
    • Publisher
      コロナ社
    • ISBN
      9784339067569
    • Related Report
      2018 Research-status Report
  • [Remarks] 東京都市大学研究者情報データベース

    • URL

      http://www.risys.gl.tcu.ac.jp/Main.php?action=profile&type=detail&tchCd=5001799

    • Related Report
      2019 Annual Research Report 2018 Research-status Report 2017 Research-status Report
  • [Remarks] 東京都市大学理工学部エネルギー化学科高分子・バイオ化学研究室<機能性バイオ分子>

    • URL

      http://www.ese.tcu.ac.jp/labs/bio

    • Related Report
      2019 Annual Research Report
  • [Remarks] 高分子・バイオ化学研究室<機能性バイオ分子>ホームページ

    • URL

      http://www.ese.tcu.ac.jp/labs/bio

    • Related Report
      2018 Research-status Report 2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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