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Studies on production of indole acetic acid by epiphytic bacteria isolated from a red alga Pyropia yezoensis

Research Project

Project/Area Number 17K07914
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic bioproduction science
Research InstitutionKumamoto University

Principal Investigator

TAKIO SUSUMU  熊本大学, くまもと水循環・減災研究教育センター, 教授 (60188109)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords紅藻スサビノリ / 海草アマモ / 付着細菌 / インドール酢酸 / インドール酢酸合成 / Neptunomonas / Pyropia yezoensis / Zostera marina / スサビノリ / 海藻付着細菌 / 植物ホルモン
Outline of Final Research Achievements

Neptunomonas sp. BPy-1 is an epiphytic bacterium isolated from in vitro culture of the red alga Pyropia
yezoensis. A related bacterium Neptunomonas sp. BZm-1 was isolated from leaves of a seagrass Zostera marina in Yatsushiro Sea. Both clones produced indole acetic acid (IAA) and promoted the growth of P. yezoensis. Bacterial IAA production was strictly dependent on tryptophan but less affected by carbon and nitrogen sources. Whole genome sequences were available for five Neptunomonas species. All of them lack two key genes in bacterial IAA biosynthetic pathway, instead some homologous genes with unrelated origin are present. To examine whether this property is present in BPy-1, whole genome sequences of BPy-1 was analyzed. The same composition of IAA biosynthetic genes was present in BPy-1.

Academic Significance and Societal Importance of the Research Achievements

スサビノリ培養株には多様な細菌が付着した状態で維持され研究に使われている。これは完全に無菌化すると異形化することが一因であるが、形態形成誘導以外の付着菌の機能は不明であった。スサビノリから分離されたNeptunomonas sp. BPy-1はスサビノリの成長を促進し、植物ホルモンの一種であるインドール酢酸(IAA)を合成した。本研究はスサビノリ付着細菌を単離培養し、IAA合成の制御様式やIAA合成遺伝子について調べたところ、従来とは異なる特徴をもつことが明らかになった。これらの研究成果はノリ養殖における安定生産や品質改善技術への応用が期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (15 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (12 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results)

  • [Int'l Joint Research] Ege University/Faculty of Engineering(トルコ)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Two ANGUSTIFOLIA genes regulate gametophore and sporophyte development in Physcomitrella patens2020

    • Author(s)
      Hashida Yoshikazu、Takechi Katsuaki、Abiru Tomomi、Yabe Noriyuki、Nagase Hiroaki、Hattori Koro、Takio Susumu、Sato Yoshikatsu、Hasebe Mitsuyasu、Tsukaya Hirokazu、Takano Hiroyoshi
    • Journal Title

      The Plant Journal

      Volume: 101 Issue: 6 Pages: 1318-1330

    • DOI

      10.1111/tpj.14592

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Production of indoleacetic acid by strains of the epiphytic bacteria Neptunomonas spp. isolated from the red alga Pyropia yezoensis and the seagrass Zostera marina2018

    • Author(s)
      Ryuya Matsuda, Midia Lestari Handayani, Hiroyuki Sasaki, Katsuaki Takechi, Hiroyoshi Takano, Susumu Takio
    • Journal Title

      Archives of Microbiology

      Volume: 200 Issue: 2 Pages: 255-265

    • DOI

      10.1007/s00203-017-1439-1

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ANGUSTIFOLIAはヒメツリガネゴケの配偶体と胞子体の両世代で細胞伸長を制御する2019

    • Author(s)
      永瀬 寛朗,橋田 芳和,武智 克彰,矢部 智幸,瀧尾 進,佐藤 良勝,長谷部 光泰,塚谷 裕一,高野 博嘉
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Superoxide generation in seeds of the seagrass Zostera marina2019

    • Author(s)
      Matsuda, R., Inamori, K., Abe, S., Takechi, K., Takano, H., Takio, S.
    • Organizer
      2019 International Conference on Climate Change, Disaster Management and Environmental Sustainability
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Production of indoleacetic acid by a atrain of epiphytic bacterium Maribacter sp. isolated from the red alga Pyropiya yezoensis2019

    • Author(s)
      Ryuya Matsuda, Shun Abe, Koki Inamori, Katsuhisa Amakura, Katsuaki Takechi, Hiroyoshi Takano, Susumu Takio
    • Organizer
      2019 International Conference on Climate Change, Disaster Management and Environmental Sustainability
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Isolation of aseptic culture of the seagrass Zostera marina using stored seeds.2019

    • Author(s)
      Aydin, E.,Takechi, K., Takano, H., Gurel, A., Takio,S.
    • Organizer
      International Marine Biotechnology Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Production of indoleacetic acid by a atrain of epiphytic bacterium Maribacter sp. isolated from the red alga Pyropiya yezoensis.2019

    • Author(s)
      Matsuda,R., Abe,S., Inamori,K., Amakura,K., Takechi,K., Takano,H., Takio, S.
    • Organizer
      International Marine Biotechnology Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 葉緑体分裂に関与するMurEと葉緑体分化に関与するMurE の相同性と変異領域2019

    • Author(s)
      加治佐 一朗,林 暁飛, 鄂 一嵐,工藤 裕美,瀧尾 進,武智 克彰,高野 博嘉
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Superoxide generation in seeds of seagrass Zostera marina.2018

    • Author(s)
      Aydin,E.,Takechi,K.,Takano,H., Takio,S.
    • Organizer
      第20回日本マリンバイオテクノロジー学会
    • Related Report
      2018 Research-status Report
  • [Presentation] シアノバクテリア及び紅藻類由来チラコイド膜における分光特性の比較2018

    • Author(s)
      廣田悠真,川上恵典,神谷信夫,瀧尾進,小澄大輔
    • Organizer
      2018年光合成セミナー
    • Related Report
      2018 Research-status Report
  • [Presentation] 藻類由来光合成超複合体における光エネルギー移動機構の解明に向けて2018

    • Author(s)
      川上恵典,廣田悠真,田原愁平,塩見潤子,眞岡孝至,瀧尾進,宮田真人,神谷信夫,小澄大輔
    • Organizer
      カロチノイド研究談話会
    • Related Report
      2018 Research-status Report
  • [Presentation] 緑色植物に 保存された,葉緑体の分化や分裂に関与するMurEの機能相補解析2018

    • Author(s)
      加治佐一朗,林暁飛, 鄂一嵐,工藤裕美,池田孝介, 瀧尾進,武智克彰,高野 博嘉
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ANGUSTIFOLIAはヒメツリガネゴケの配偶体と胞子体の両世代で細胞伸長を制御する2018

    • Author(s)
      永瀬寛朗,橋田芳和,武智克彰,矢部智幸,瀧尾進,佐藤良勝,長谷部光泰,塚谷裕一,高野博嘉
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 紅藻スサビノリの栄養欠乏によるフィコビリソーム分解2017

    • Author(s)
      瀧尾 進,東遥香,武智克彰,高野博嘉
    • Organizer
      第19回日本マリンバイオテクノロジー学会
    • Related Report
      2017 Research-status Report
    • Invited

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Published: 2017-04-28   Modified: 2021-02-19  

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