• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Growth characterization of histamine producing Morganella psychrotolerans and bacteriophage therapy in seafood

Research Project

Project/Area Number 17K07931
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic life science
Research InstitutionHokkaido University

Principal Investigator

Yamazaki Koji  北海道大学, 水産科学研究院, 准教授 (40250500)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsヒスタミン食中毒 / Morganella / 水産食品 / バクテリオファージ / ヒスタミン / 微生物制御 / 食中毒 / モルガネラ / モルがネラ
Outline of Final Research Achievements

This study was investigated the contamination status of Morganella psychrotolerans in detail seafood product in Japan, and the characterization and utilization of a newly isolated bacteriophage, MPV5, for preventing the growth and histamine accumulation. M.psychrotolerans was widely distributed in retail seafood product. An effective bacteriophage, MPV5, was successfully isolated from river water in this study. The viable count and histamine accumulation of M.psychrotolerans inhibited in broth and tuna meat by the phage MPV5 treatment. The combined treatment of MPV5 and sodium acetate enhanced to prevent the growth and histamine accumulation in tuna meat. These results suggest that phage treatment with MPV5 could be an effective biocontrol method to prevent the histamine poisoning by M.psychrotolerans in seafood.

Academic Significance and Societal Importance of the Research Achievements

ヒスタミンによる食中毒は日本だけでなく,水産物取扱いの衛生状態が良くない欧米でも頻発している。本研究では低温増殖性のM. psychrotoleransがヒスタミン中毒を視点に入れた場合の新しい制御対象細菌であることを示し,またヒスタミン中毒を予防するための手法としてバクテリオファージを利用した新しい微生物制御法の有効性を示した。したがって,水産食品の食品安全性を向上させる技術開発ための基礎的知見として貢献度は大きい。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (8 results)

All 2020 2019 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Histamine production behaviors of a psychrotolerant histamine-producer, Morganella psychrotolerans, at various environmental conditions2020

    • Author(s)
      Wang, D., S. Yamaki, Y. Kawai, and K. Yamazaki
    • Journal Title

      Current Microbiology

      Volume: 77 Issue: 3 Pages: 460-467

    • DOI

      10.1007/s00284-019-01853-y

    • NAID

      120006950862

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sanitizing efficacy and antimicrobial mechanism of peracetic acid against histamine producing bacterium, Morganella psychrotolerans,2020

    • Author(s)
      Wang, D., S. Yamaki, Y. Kawai, and K. Yamazaki
    • Journal Title

      LWT-Food Science and Technology

      Volume: 126 Pages: 109263-109263

    • DOI

      10.1016/j.lwt.2020.109263

    • NAID

      120007034042

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] Histamine production of Morganella psychrotolerans and antibacterial effect of peracetic acid to bacterial cells2019

    • Author(s)
      Wang Di, Yamaki Shogo, Kawai Yuji, Yamazaki Koji
    • Organizer
      日本食品科学工学会第66回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] バクテリオファージと有機酸塩を利用したヒスタミン産生菌 Morganella psychrotolerans の発育抑制2019

    • Author(s)
      櫻井 友輔, 山木 将悟, 川合 祐史, 山崎 浩司
    • Organizer
      日本食品科学工学会第66回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Morganella psychrotoleransに感染するバクテリオファージの分離と性状2018

    • Author(s)
      山木将悟,加藤莉子,川合祐史,山崎浩司
    • Organizer
      日本食品科学工学会第65回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Growth behavior and control of Morganella psychrotolerans related to scombroid food poisoning in seafood2018

    • Author(s)
      Di Wang, Shogo Yamaki, Riko Kato, Yuji Kawai, Koji Yamazaki
    • Organizer
      The 8th Academic Exchange for Collaborative Research Between ETHZ and Hokkaido University
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Effects of environmental factors on histamine production in psychrotolerant Morganella psychrotolerans2018

    • Author(s)
      Di Wang, Shogo Yamaki, Yuji Kawai, Koji Yamazaki
    • Organizer
      平成30年日本食品科学工学会北海道支部大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 低温性ヒスタミン生成菌Morganella psychrotoleransの水産食品における分布と発育挙動2017

    • Author(s)
      加藤莉子,王 迪, 山木将悟, 川合祐史, 山﨑浩司
    • Organizer
      第38回日本食品微生物学会学術総会
    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi