Molecular mechanisms of high light tolerance in microalgae
| Project/Area Number |
17K07945
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Aquatic life science
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| Research Institution | Teikyo University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ユーグレナ / 強光ストレス / 微細藻類 / カロテノイド / 光走性 / 走光性 / 鞭毛藻 / 単細胞 / 強光 / 光合成活性 / 青色光 / 赤色光 / 低温ストレス / P450 / ジャスモン酸 / 微生物 / バイオマス / 環境 / 遺伝子 / 植物 |
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| Outline of Final Research Achievements |
Euglena gracilis, a unicellular phytoflagellate, is a potential feedstock for renewal energy. In outdoor open-pond cultivation, the direct solar radiation decreases the productivity of this alga. Here we cloned carotenoid biosynthetic genes such as phytoene desaturase genes and ζ-carotene desaturase gene from E. gracilis, and analyzed their expression level and their physiological functions under the strong light condition using RNAi knocked down lines. This study showed that excess light-stress decreased their cell growth, and changed an accumulation of specific carotenoid species in the cells of E. gracilis as well as their change of phototaxis. We concluded that E. gracilis enhanced tolerance to the light stress by both “a rapid response for cell movement” and “a slower response for change of carotenoid metabolism” mediated by carotenoids.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により、ユーグレナという系統進化上特異な分類群に属するユニークな生物は、強光ストレス環境下でカロテノイドの全体量を変化させるのみならず、特定のカロテノイド分子種の蓄積量や分布を変化させる分子機構を持つことがはじめて明らかになった。さらに、本研究により、比較的弱い強度の青色光や赤色光をユーグレナ細胞にあらかじめ一定時間照射することにより、β-カロテンの蓄積を通じてその後の強光ストレスによる光合成活性の低下を緩和する可能性が示唆された。このことは、将来、屋外でのユーグレナ細胞の大量培養における太陽の直射光によるストレス緩和への実用上の応用の可能性があると考えられる。
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Report
(4 results)
Research Products
(40 results)
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[Presentation] Blocking of carotenoid biosynthesis caused a defect in the normal eyespot formation and resulted in a loss of phototaxis of Euglena gracilis2019
Author(s)
Kato Shota, Ozasa Kazunari, Maeda Mizuo, Tanno Yuri, Higuchi Mieko, Numata Keiji, Kodama Yutaka, Sato Mayuko, Toyooka Kiminori, Nam Hong Gil, Shinomura Tomoko
Organizer
Japan-Taiwan Plant Biology (JTPB2019)
Related Report
Int'l Joint Research
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[Presentation] Gene Expression profiling and Genetic transformation trials on Pediastrum duplex, a model for photoregulation of life cycle2018
Author(s)
Sridharan Harshavardhini, Kato Shota, Tsuchikane Yuki, Sekimoto Hiroyuki, Nagata Noriko, Suzuki Tomohiro, Kodama Yutaka, Aiso Haruna, Miyamoto Koji, Matsuoka Keita, Asahina Masashi, Shinomura Tomoko
Organizer
日本植物学会第82回大会
Related Report
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