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Efficient production of camptothecin by Ophiorrhiza pumila under controlled environmental

Research Project

Project/Area Number 17K08018
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Agricultural environmental engineering/Agricultural information engineering
Research InstitutionChiba University

Principal Investigator

Hikosaka Shoko  千葉大学, 大学院園芸学研究科, 准教授 (50345188)

Co-Investigator(Kenkyū-buntansha) 石神 靖弘  高崎健康福祉大学, 農学部, 准教授 (50361415)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords抗がん成分 / アルカロイド / 光合成・蒸散速度 / 光環境 / 培養液温度 / 培養液浸透圧 / 光合成有効放射束密度 / カンプトテシン / 光合成速度 / 蒸散速度 / 同化箱 / UVストレス / 浸透ストレス / 明暗周期 / PPFD / 赤色青色光比 / 培養液濃度 / 形態形成 / 相対湿度 / 成長速度 / 明期 / 花芽分化 / 湿度 / 光強度 / 生物環境調節 / 局所環境制御 / 抗がん剤 / ストレス応答
Outline of Final Research Achievements

1. Basic characteristics of photosynthesis: Using an assimilation improved last year, the effects of PPFD on photosynthesis and transpiration rate were investigated. Although various combinations were studied, the photosynthetic rate decreased within 1 hour under PPFD 300. On the other hand, it was clarified that the increase in the daily cumulative photosynthesis amount by extension of the light period seemed effective to promotion of growth and flower bud differentiation. This was considered to be a suitable condition for producing a large number of seeds with a high concentration of CPT.
2. Optimal environmental conditions for CPT production: Polyethylene glycol (PEG) was added to the nutrient solution to increase the osmotic pressure around the roots for 3 days. The osmotic stress treatment for 2 and 3 days increased the CPT concentration per dry weight of the stems and roots, suggesting that water absorption stress from the roots is effective in increasing the CPT concentration.

Academic Significance and Societal Importance of the Research Achievements

主要な抗がん剤などに利用される植物アルカロイドの一つ、カンプトテシン(CPT)を生産する小型草本植物「チャボイナモリ」の生理生態を調査し、人工環境下で効率生産する好適環境条件についての基礎的知見を得た点で学術的意義がある。特に本研究では、野生では高温多湿条件下で生育するチャボイナモリの生育およびCPT蓄積に適した環境条件をそれぞれ明らかにした。これによりこれまで大型木本植物から抽出されてきたCPTを短期間かつ安価に抽出できることから、大幅な期間とコスト削減につながる。また、人工光型植物工場で生産することで、品質安定化や多段・高密度生産が可能となり、製薬コストの削減によって社会貢献につながる。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (8 results)

All 2021 2020 2019 2018

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (5 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Chromosome-level genome assembly of Ophiorrhiza pumila reveals the evolution of camptothecin biosynthesis2021

    • Author(s)
      Rai Amit、Hirakawa Hideki、Nakabayashi Ryo、Kikuchi Shinji、Hayashi Koki、Rai Megha、Tsugawa Hiroshi、Nakaya Taiki、Mori Tetsuya、Nagasaki Hideki、Fukushi Runa、Kusuya Yoko、Takahashi Hiroki、Uchiyama Hiroshi、Toyoda Atsushi、Hikosaka Shoko、Goto Eiji、Saito Kazuki、Yamazaki Mami
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 405-405

    • DOI

      10.1038/s41467-020-20508-2

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effects of photosynthetic photon flux density and light period on growth and camptothecin accumulation of <i>Ophiorrhiza pumila</i> under controlled environments2020

    • Author(s)
      Ji-Yoon LEE、SHIMANO Akimasa、HIKOSAKA Shoko、ISHIGAMI Yasuhiro、GOTO Eiji
    • Journal Title

      Journal of Agricultural Meteorology

      Volume: 76 Issue: 4 Pages: 180-187

    • DOI

      10.2480/agrmet.D-20-00026

    • NAID

      130007925128

    • ISSN
      0021-8588, 1881-0136
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of Concentration and Temperature of Nutrient Solution on Growth and Camptothecin Accumulation of Ophiorrhiza pumila2020

    • Author(s)
      Lee Ji-Yoon、Hiyama Miki、Hikosaka Shoko、Goto Eiji
    • Journal Title

      Plants

      Volume: 9 Issue: 6 Pages: 793-793

    • DOI

      10.3390/plants9060793

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Measurement of whole plant gas exchange rates of Ophiorrhiza pumila using an open-type assimilation chamber2019

    • Author(s)
      Lee JY, Hikosaka S, Ishigami Y, Goto E
    • Organizer
      International Symposium on Advanced Technologies and Management for Innovation (Greensys)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Effects of nutrient solution concentration on growth and camptothecin content of Ophiorrhiza pumila2019

    • Author(s)
      Lee JY, Hikosaka S, Goto E
    • Organizer
      The annual meeting of Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists
    • Related Report
      2019 Research-status Report
  • [Presentation] Whole plant photosynthetic and transpiration rates of Ophiorrhiza pumila measured using an open-type assimilation chamber2019

    • Author(s)
      Lee, J., Hikosaka, H., Ishigami, Y., Goto, E
    • Organizer
      (ISAM2018) International Symposium on Agricultural Meteorology 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Measurement of whole plant gas exchange rates of Ophiorrhiza pumila using an open-type assimilation chamber2019

    • Author(s)
      Lee, J., Hikosaka, H., Ishigami, Y., Goto, E
    • Organizer
      (GreenSys 2019) International Symposium on Advanced Technologies and Management for Innovative Greenhouses
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of an open-type assimilation chamber to measure photosynthetic and transpiration rates of Ophiorrhiza pumila under controlled environments2018

    • Author(s)
      Lee, J., Hikosaka, H., Ishigami, Y., Goto, E
    • Organizer
      Asian conference for protected horticulture and plant factory
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2022-01-27  

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