| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Attempts have been made to produce germ line chimera (GLC) by transferring germline stem cells (gSCs) from 2-9 days-old chick embryos. GLC was produced in all cases except when gSCs from female 9 days-old embryos have been transferred to the recipients.In the present study, therefore, RNA was extracted from gSCs and RNA-seq was conducted to examine the temporal changes of mRNA pattern of gSCs associated with early embryonic development.
The results indicate that 1) expression of genes related to pluripotency have been down-regulated by 5 days in both sexes. 2) expression of genes related to germ cells were down-regulated throughout the examination periods 3) No significant differences in the expression of genes related to meiosis in both sexes.
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