Adipogenic - myogenic regulation leads effective meat production

• Project/Area Number: 17K08065
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Kyushu University
• Principal Investigator: Nakamura Mako 九州大学, 農学研究院, 准教授 (80452746)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: Pax7 / Adipogenesis / Cell specific expression / Lipid droplet / Inhibition / PPARgamma / Immunoprecipitation / Preadipocytes / preadipocytes / transdifferentiation / transcription / repression / myoblasts / 筋肉細胞 / 脂肪細胞 / 再分化 / 肉質予測 / 脂肪交雑比率

Outline of Final Research Achievements

Myogenesis is precisely proceeded by myogenic regulatory factors. Myogenic stem cells are activated, proliferated and fused into a multinuclear myofiber. Pax7, paired box 7, one of the earliest markers during myogenesis. It has been reported that Pax7 regulates the muscle marker genes, Myf5 and MyoD toward differentiation. The possible roles of Pax7 in myogenic cells have been well researched. However, it has not yet been clarified if Pax7 itself is able to induce myogenic fate in non-myogenic lineage cells. In this study, we performed experiments using stably expressed Pax7 in 3T3-L1 preadipocytes to elucidate if Pax7 inhibits adipogenesis. We found that Pax7 represses adipogenic markers and prevents differentiation. These cells showed decreased expression of PDGFRalpha, PPARganma and Fabp4 and inhibited forming lipid droplets.

Academic Significance and Societal Importance of the Research Achievements

現在の食肉生産は、長期間の肥育の結果、屠殺後に高品質であれば付加価値が望めるが低品質の場合は原価の回収が不能となる。そのための安全策として環境負荷の高い穀物飼料に依存することで脂肪交雑率を維持するため、飼料経費や施設・設備の維持の点からも問題となっている。食肉はきめ細かさ、硬さ、脂肪交雑率といった基準で評価されるが、その肉質は形成される筋肉線維の太さ、脂肪細胞の割合、結合組織（線維芽細胞）の割合等に影響される。そこで、筋肉と脂肪の割合を調節できることを目指し、両者が組織形成に与える影響を細胞レベルで検証した。

Research Products

• [Int'l Joint Research] INSERM/Universite de montpellier(フランス)
• [Journal Article] Correlation between skeletal muscle fiber type and free amino acid levels in Japanese Black steers (2019)
  • Author(s): Mashima Daisuke、Oka Yoshiaki、Gotoh Takafumi、Tomonaga Shozo、Sawano Shoko、Nakamura Mako、Tatsumi Ryuichi、Mizunoya Wataru
  • Journal Title: Animal Science Journal Volume: 90 Issue: 4 Pages: 604-609
  • DOI: 10.1111/asj.13185
  • Peer Reviewed / Open Access
• [Journal Article] Increase in muscle endurance in mice by dietary Yamabushitake mushroom ( Hericium erinaceus ) possibly via activation of PPAR δ (2019)
  • Author(s): Komiya Yusuke、Nakamura Toshiya、Ishii Momoko、Shimizu Kuniyoshi、Hiraki Eri、Kawabata Fuminori、Nakamura Mako、Tatsumi Ryuichi、Ikeuchi Yoshihide、Mizunoya Wataru
  • Journal Title: Animal Science Journal Volume: - Issue: 6 Pages: 781-789
  • DOI: 10.1111/asj.13199
  • Peer Reviewed / Open Access
• [Journal Article] Effect of Gender, Rearing, and Cooking on the Metabolomic Profile of Porcine Muscles (2019)
  • Author(s): Sawano Shoko、Oza Keishi、Murakami Tetsuya、Nakamura Mako、Tatsumi Ryuichi、Mizunoya Wataru
  • Journal Title: Metabolites Volume: 10 Issue: 1 Pages: 10-10
  • DOI: 10.3390/metabo10010010
  • Peer Reviewed / Open Access
• [Journal Article] Isolation and Purification of Satellite Cells from Young Rats by Percoll Density Gradient Centrifugation (2018)
  • Author(s): Matsuyoshi Yuji、Akahoshi Mariko、Nakamura Mako、Tatsumi Ryuichi、Mizunoya Wataru
  • Journal Title: Methods in Molecular Biology Volume: 1889 Pages: 81-93
  • DOI: 10.1007/978-1-4939-8897-6_6
  • ISBN: 9781493988969, 9781493988976
  • Peer Reviewed
• [Journal Article] Paired box 7 inhibits differentiation in 3T3-L1 preadipocytes (2018)
  • Author(s): Izumi Wakana、Takuma Yuko、Ebihara Ryo、Mizunoya Wataru、Tatsumi Ryuichi、Nakamura Mako
  • Journal Title: Animal Science Journal Volume: 89 Issue: 8 Pages: 1214-1219
  • DOI: 10.1111/asj.13050
  • Peer Reviewed
• [Journal Article] Fluorescence microscopy data on expression of Paired Box Transcription Factor 7 in skeletal muscle of APOBEC2 knockout mice (2018)
  • Author(s): Ohtsubo Hideaki、Sato Yusuke、Matsuyoshi Yuji、Suzuki Takahiro、Mizunoya Wataru、Nakamura Mako、Tatsumi Ryuichi、Ikeuchi Yoshihide
  • Journal Title: Data in Brief Volume: 17 Pages: 1348-1351
  • DOI: 10.1016/j.dib.2018.02.063
  • Peer Reviewed / Open Access
• [Journal Article] Apobec2 deficiency causes mitochondrial defects and mitophagy in skeletal muscle (2018)
  • Author(s): Sato Yusuke、Ohtsubo Hideaki、Nihei Naohiro、Kaneko Takane、Sato Yoriko、Adachi Shin-Ichi、Kondo Shinji、Nakamura Mako、Mizunoya Wataru、Iida Hiroshi、Tatsumi Ryuichi、Rada Cristina、Yoshizawa Fumiaki
  • Journal Title: FASEB Journal Volume: 32 Issue: 3 Pages: 1428-1439
  • DOI: 10.1096/fj.201700493r
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 食品としての油脂が骨格筋の代謝特性に与える影響と筋線維タイプとの関連 (2018)
  • Author(s): 小宮佑介, 丸山アレクサンデル, 渡辺裕介, 内田直愛, 大津翔平, 小林千亜暉, 横山壱成, 鈴木貴弘, 中村真子, 辰巳隆一, 池内義秀, 水野谷航, 有原圭三
  • Journal Title: 栄養生理研究会報 Volume: 62 Pages: 9-17
  • Peer Reviewed
• [Journal Article] APOBEC2 negatively regulates myoblast differentiation in muscle regeneration (2017)
  • Author(s): Hideaki Ohtsubo, Yusuke Sato, Takahiro Suzuki, Wataru Mizunoya, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi
  • Journal Title: International Journal of Biochemistry and Cell Biology Volume: 85 Pages: 91-101
  • DOI: 10.1016/j.biocel.2017.02.005
  • Peer Reviewed
• [Journal Article] Data supporting possible implication of APOBEC2 in self-renewal functions of myogenic stem satellite cells: toward understanding the negative regulation of myoblast differentiation. (2017)
  • Author(s): Ohtsubo, H., Sato Y., Suzuki, T., Mizunoya, W., Nakamura, M., Tatsumi, R., and Ikeuchi, Y.
  • Journal Title: Data in Brief Volume: 12 Pages: 269-273
  • DOI: 10.1016/j.dib.2017.03.051
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mouse soleus (slow) and muscle shows greater intramyocellular lipid droplet accumulateon than EDL (fast) muscle: fiber type-specific analysis (2017)
  • Author(s): Komiya Y, Sawano S, Mashima D, Ichitsubo R, Nakamura M, Tatsumi R, Ikeuchi Y, Mizunoya W
  • Journal Title: Journal of Muscle Research and Cell Motility Volume: 印刷中 Issue: 2 Pages: 163-173
  • DOI: 10.1007/s10974-017-9468-6
  • Peer Reviewed / Open Access
• [Journal Article] Slow-Myofiber Commitment by Semaphorin 3A Secreted from Myogenic Stem Cell (2017)
  • Author(s): R Tatsumi,T Suzuki, Mai-Khoi Q. Do, Y Ohya, JE. Anderson, A Shibata, M Kawaguchi, S Ohya, H Ohtsubo, W Mizunoya, S Sawano, Y Komiya, R Ichitsubo, K Ojima, S Nishimatsu, T Nohno, Y Ohsawa, Y Sunada, M Nakamura, M Furuse, Y Ikeuchi, T Nishimura, T Yagi, and R E. Allen
  • Journal Title: STEM CELLS Volume: 35 Issue: 7 Pages: 1815-1834
  • DOI: 10.1002/stem.2639
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Improving meat quality by regulating myogenic and adipogenic cell fate -A possible role of Paired box 7 (Pax7) during adipogenic differentiation (2019)
  • Author(s): Wakana Izumi, Yuko Takuma, Wataru Mizunoya, Ryuichi Tatsumi and Mako Nakamura
  • Organizer: International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia (AFELiSA)
  • Int'l Joint Research
• [Presentation] The serum-free media allows chick gizzard smooth muscle cells in differentiated state. (2019)
  • Author(s): Kosuke Tokunaga, Shota Akimoto, Wataru Mizunoya, Ryuichi Tatsumi and Mako Nakamura
  • Organizer: International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia (AFELiSA)
  • Int'l Joint Research
• [Presentation] HGF nitration abolishes myogenic stem satellite cell activation (2019)
  • Author(s): Hirochika Kido, Nana Imatomi, Shoko Okuda, Yoshitaka Manabe, Yuji Matsuyosi, Alaa Magdi Hassanein Elgaabari, Takashi Nakashima, Shoko Sawano, Wataru Mizunoya, Mako Nakamura, and Ryuichi Tatsumi
  • Organizer: International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia (AFELiSA)
  • Int'l Joint Research
• [Presentation] ニワトリ胚砂嚢を用いた分化型平滑筋細胞培養法の開発 (2019)
  • Author(s): 徳永亘祐、水野谷航、辰巳隆一、中村真子
  • Organizer: 家禽学会2019秋季大会
• [Presentation] 牛肉の風味に影響を与える脂肪以外の因子の解明―筋線維タイプに着目した解析― (2019)
  • Author(s): 間島大介 ・岩井芳夫・後藤貴文・澤野祥子・辰巳隆一・中村真子・水野谷航
  • Organizer: 日本畜産学会第125回大会
• [Presentation] Pax7が脂肪前駆細胞分化の運命決定に与える影響 (2019)
  • Author(s): 泉和佳奈・水野谷航・辰己隆一・中村真子
  • Organizer: 日本畜産学会第125回大会
• [Presentation] Sema3A依存的な遅筋型筋線維形成機構の食品機能学的制御： リンゴポリフェノールによる促進と活性成分の同定 (2019)
  • Author(s): 松吉祐児・赤星眞理子・大宅駿平・水野谷航・澤野祥子・中村真子・辰巳隆一
  • Organizer: 日本畜産学会第125回大会
• [Presentation] 筋幹細胞分泌因子Sema3A による遅筋型筋線維形成誘導：ヒラメ筋の損傷・再生実験による実証 (2019)
  • Author(s): 大宅駿平・松吉祐児・川口舞・水野谷航・澤野祥子・中村真子・辰巳隆一
  • Organizer: 日本畜産学会第125回大会
• [Presentation] 大規模豚舎を用いたブタの多頭飼育が肉質に与える影響 (2018)
  • Author(s): 増原夏海・荒川拓実・村上徹哉・山口勇史・間島大介・中村真子・辰巳隆一・水野谷航
  • Organizer: 第11回日本暖地畜産学会長崎大会
• [Presentation] 大規模豚舎を用いたブタの多頭飼育が飼育中の運動量に与える影響 (2018)
  • Author(s): 荒川拓実・増原夏海・村上徹哉・山口勇史・間島大介・中村真子・辰巳隆一・水野谷航
  • Organizer: 第11回日本暖地畜産学会長崎大会
• [Presentation] Percoll密度勾配遠心分離法を用いた若齢ラット由来の筋衛星細胞精製方法の確立 (2018)
  • Author(s): 松吉 祐児、赤星 真理子、中村 真子、辰巳 隆一、水野谷 航
  • Organizer: 日本筋学会第４回学術集会「筋研究の新たな地平へ-基礎と臨床の融合-」
• [Presentation] Slow-fiber commitment by Sema3A secretion from myogenic stem satellite cells. (2018)
  • Author(s): Tatsumi R., Suzuki T., Kawaguchi M., Do M.-K. Q., Ohya S., Matsuyoshi Y., Ohya Y., Anderson J. A., Mizunoya W., Sawano S., Sakata K., Nakamura M., Allen R. E.
  • Organizer: 2018 FASEB Science Research Conference on “Skeletal Muscle Satellite Cells and Regeneration”
  • Int'l Joint Research
• [Presentation] ブタへの継続的運動負荷は筋線維タイプおよび肉質を変化させる (2018)
  • Author(s): 水野谷航、大座啓史、村上徹哉、笠正二郎、間島大介、中村真子、辰巳隆一
  • Organizer: 第72回日本栄養・食糧学会大会
• [Presentation] Paired box 7 inhibits adipogenic differentiation -A possible role of Pax7 in non-myogenic cells. (2018)
  • Author(s): Mako Nakamura, Wakana Izumi, Wataru Mizunoya, Ryuichi Tatsumi
  • Organizer: Avian Model Systems 10
• [Presentation] 筋幹細胞分泌因子Sema3Aによる筋線維型制御に関する研究：マウス成長過程における解析 (2018)
  • Author(s): 川口 舞、坂田拡太、松吉祐児、水野谷航、中村真子、辰巳隆一
  • Organizer: 日本畜産学会 第124回大会
• [Presentation] Nudt7 overexpression acyivates endogenous Nudt7 and MyHC1 mRNA expression in C2C12 myoblasts (2018)
  • Author(s): Ryo Ebihara, Wataru Mizunoya, Ryuichi Tatsumi, Mako Nakamura
  • Organizer: 日本畜産学会 第124回大会
• [Presentation] マウス骨格筋の再生に伴う筋線維タイプ組成の経時的解析 (2018)
  • Author(s): 市坪里穂、澤野祥子、辰巳隆一、中村真子、水野谷航
  • Organizer: 日本畜産学会 第124回大会
• [Presentation] Possible implication of Nudt7 in C2C12 myoblast differentiation (2017)
  • Author(s): Ebihara R., Choe S.-K., Mizunoya W., Tatsumi R., Nakamura M.
  • Organizer: International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia, 2017 (AFELiSA'17)
  • Int'l Joint Research
• [Presentation] 運動による筋線維タイプの変化によって豚肉の肉質がどう変わるか？ (2017)
  • Author(s): 澤野 祥子、大座 啓史、辰巳 隆一、中村 真子、水野谷 航
  • Organizer: 2017年度生命科学系学会合同年次大会（ConBio2017）
• [Remarks] 九州大学-研究者情報
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/K003166/research.html