| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
We are aiming to design a neutralizing antibody against escape mutant strains and/or multiple HA subtypes of influenza A viruses. In this study, we tried to modify the binding ability of S139/1, a cross-reactive neutralizing monoclonal antibody against HAs, using the binding free energy obtained from molecular dynamics simulations as an indicator. For the HA from A/Victoria/3/75 (H3N2) used in this study, only amino acid substitutions on S139/1 was unable to restore the recogniton ability of S139/1 against escape mutants, suggesting that the replacement of an entire CDR loop are required.
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