| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Bovine respiratory disease complex (BRDC) is a major disease affecting the global cattle industry. Bovine respiratory syncytial virus (BRSV) is a primary causative agent of BRDC and Pasteurella multocida (PM) was the most common bacterial agent paired with virus in BRDC. We established bovine respiratory epithelial cell (BRECs) from trachea (bTECs), bronchus (bBECs) and lung epithelial cells (bLECs) of adult Japanese black cattle and performed comparative analyses to examine the interaction between BRSV and PM. PM adherences to BRSV-infected bBECs and bLECs were significantly higher (p < 0.05) than those on BRSV-infected bTECs. Interestingly, PM adherence on uninfected bTECs was significantly higher than those on bBECs and bLECs.
These results suggested that BRSV infection plays an important role for bacterial adherences in lung. While, upper respiratory epithelial cells efficiently capture PM as a “gateway” of respiratory tract.
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