Production of the novel hepatitis E virus (HEV) and examination of the possibility of the zoonotic infection
| Project/Area Number |
17K08090
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Li Tiancheng 国立感染症研究所, ウイルス第二部, 主任研究官 (90370957)
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| Co-Investigator(Kenkyū-buntansha) |
網 康至 国立感染症研究所, 動物管理室, 主任研究官 (10202699)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | HEV / 人獣共通感染症 / リバースジェネティック法 / リバースジェネティック / Rabbit HEV / 動物モデル / G5 HEV / 人獣共通感染症の可能性 / 細胞培養 / E型肝炎 / Hepatitis E virus |
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| Outline of Final Research Achievements |
In this study, we used a reverse genetics system to generate HEV-5 and examined the possibility of zoonotic infection. Infectious HEV-5 was produced using a reverse genetics system, and the antigenicity was identical to that of human HEVs. Transmission of HEV-5 to primates was confirmed by an experimental infection, providing evidence of the possibility of zoonotic infection by HEV-5.
Further, rabbit HEV was also generated by reverse genetics system and the recovered viruses was capable of infecting rabbits negative for anti-HEV antibody by intravenous and oral inoculation. In addition, transmission of rabbit HEV to rabbits caused persistent infection, suggesting that the virus-infected rabbit could be an animal model for virus-induced hepatitis.
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| Academic Significance and Societal Importance of the Research Achievements |
新型HEV-5をリバースジェネティック法により世界は作製に成功した。また、HEV-5が霊長類のカニクイザルに感染することにより,HEV-5の人獣共通感染症の可能性が示唆された。この発見はE型肝炎対策の策定に非常に有意義なことである。また、rabbit HEVがウサギに持続感染を引き起こすことは動物モデルの樹立にも大いに期待できる。
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Peppelenbosch MP, Zhao J, Pan Q. The RNA genome of hepatitis E virus robustly triggers an antiviral interferon response2017
Author(s)
Wang W, Wang Y, Qu C, Wang S, Zhou J, Cao W, Xu L, Ma B, Hakim MS, Yin Y, Li TC, Peppelenbosch MP, Zhao J, Pan Q.
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Journal Title
Hepatology
Volume: 67(6)
Issue: 6
Pages: 2096-2112
DOI
Related Report
Peer Reviewed
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[Journal Article] Genetic and physicochemical analyses of a novel ferret hepatitis E virus, and clinical signs of infection after birth2017
Author(s)
Li T.C., Yoshizaki S., Kataoka M., Ami Y., Suzaki Y., Doan Y.H., Haga K., Ishii K., Takeda N. and Wakita T.
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Journal Title
Infection, Genetics and Evolution
Volume: 25
Pages: 153-159
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] w Keskes Melaku, Boldbaatar Bazartseren, Takeda N, Wakita T.2017
Author(s)
Li TC, Yoshizaki S, Zhou X, Sentsui H, Kazuya Shirato, Matsuyama S, Simenew Keskes Melaku, Boldbaatar Bazartseren, Takeda N, Wakita T.
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Journal Title
J Virol Methods.
Volume: 21;246
Pages: 34-37
DOI
Related Report
Peer Reviewed
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