Development of fundamental technologies for elucidation of the nature of Reston ebolavirus infection

• Project/Area Number: 17K08091
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Fukushi Shuetsu 国立感染症研究所, ウイルス第一部, 室長 (80373398)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: レストンエボラウイルス / 抗体検出 / 遺伝子検出 / モノクローナル抗体 / ウイルス感染症 / 血清診断 / 公衆衛生

Outline of Final Research Achievements

The purpose of this research project is to develop fundamental technologies for elucidation of the nature of Reston ebolavirus (REBOV) infection. I analyzed genome sequences of REBOV detected from cynomolgus monkeys in the Philippines and designed novel primer/probes for a real-time PCR method to detect REBOV infection. This method is evaluated using RNA samples obtained from REBOV infected monkeys. For a serological testing, I developed competitive ELISA for detecting serum anti-REBOV antibody using REBOV-NP specific monoclonal antibody. In addition, the REBOV-GP expression system was established. It might be a useful material for developing anti-GP monoclonal antibodies.

Academic Significance and Societal Importance of the Research Achievements

本研究により開発される抗体および遺伝子検出法は、現在、サル検疫施設において行われている検査に加え、レストンエボラウイルス感染の確定診断法として適用できる可能があり、公衆衛生対策への技術的貢献が期待できる。

Research Products

• [Int'l Joint Research] CSIRO(オーストラリア)
• [Int'l Joint Research] RITM(フィリピン)
• [Int'l Joint Research] RITM(フィリピン)
• [Int'l Joint Research] CSIRO(オーストラリア)
• [Int'l Joint Research] Research Institute for Tropical Medicine/INA Research Philippines(Philippines)
• [Int'l Joint Research] Australian Animal Health Laboratory(Australia)
• [Journal Article] Reston Ebolavirus in Macaques (2020)
  • Author(s): Ina L. Smith, Catalino Demetria, and Shuetsu Fukushi
  • Journal Title: Neglected Diseases in Monkeys, Springer Nature Switzerland AG 2020 Volume: 2020 Pages: 275-289
  • DOI: 10.1007/978-3-030-52283-4_12
  • ISBN: 9783030522827, 9783030522834
  • Int'l Joint Research
• [Journal Article] Reemergence of Reston ebolavirus in Cynomolgus Monkeys, the Philippines, 2015. (2018)
  • Author(s): Demetria C, Smith I, Tan T, Villarico D, Simon EM, Centeno R, Tachedjian M, Taniguchi S, Shimojima M, Miranda NLJ, Miranda ME, Rondina MMR, Capistrano R, Tandoc A 3rd, Marsh G, Eagles D, Cruz R, Fukushi S.
  • Journal Title: Emerg Infect Dis. Volume: 24 Issue: 7 Pages: 1285-1291
  • DOI: 10.3201/eid2407.171234
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Re-emergence of Reston Ebola Virus in Cynomolgus Monkeys in the Philippines, 2015 (2018)
  • Author(s): Catalino Demetria, Ina Smith, Titus Tan, Daniel Villarico, Edson Michael Simon, Rex Centeno, Mary Tachedjian, Satoshi Taniguchi, Masayuki Shimojima, Noel Lee J. Miranda, Mary Elizabeth Miranda, Melissa Marie R. Rondina , Rowena Capistrano, Amado Tandoc III, Glenn Marsh, Debbie Eagles, Ramses Cruz and Shuetsu Fukushi
  • Journal Title: Emerging Infectious Diseases Volume: 印刷中
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Re-emergence of Reston Ebola Virus in Cynomolgus Monkeys in the Philippines, 2015 (2018)
  • Author(s): Shuetsu Fukushi, Satoshi Taniguchi, Shumpei Watanabe, Takeshi Kurosu, Tomoki Yoshikawa, Masayuki Shimojima, Shigeru Morikawa, Masayuki Saijo
  • Organizer: 第66回 日本ウイルス学会学術集会