| Project/Area Number |
17K08093
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Iwate University |
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Principal Investigator |
Hoshino Yuki 岩手大学, 農学部, 准教授 (80523323)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 犬マクロファージ / RNA-seq / M2型抑制 / M2抑制 / 遺伝子の網羅的解析 / 無血清培地 / 犬血清 / 移行上皮癌 / マクロファージ / 線維芽細胞 / CD11b / 犬 / 癌 |
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| Outline of Final Research Achievements |
Some methods for inducing Mφ-like cells from canine PBMC were performed, and Mφ-like cells were evaluated. All Mφ-like cells derived from PBMC appeared to be morphologically, functionally, and genetically Mφ. It seems that the method using an inducer in the FBS-free medium can collect Mφ most efficiently. RNA-seq was performed as a comprehensive gene analysis of these Mφs, and the expressed genes were compared.
When the obtained Mφ was co-cultured with canine transitional cell carcinoma cells, it was considered that the antitumor effect might be enhanced. This study suggests that Mφ-like cells induced in FBS-free medium can be sufficiently used for future experiments and may enhance the antitumor effect on canine tumors.
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| Academic Significance and Societal Importance of the Research Achievements |
犬のマクロファージを効率よく分離・培養する方法を開発し、得られたマクロファージの正常解析を実施した。これらのマクロファージの発現遺伝子はマウス・ラット・ヒトと異なる部分が多く認められた。また、マクロファージには間接的な抗腫瘍効果があることが予想された。腫瘍微小環境における犬のマクロファージの役割が少しづつ判明してきている。
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