Effect of glucocorticoid receptor genotype on allergy

• Project/Area Number: 17K08129
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: Koyanagi Madoka 日本獣医生命科学大学, 応用生命科学部, 講師 (00543399)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 心理的ストレス / アレルギー / ストレス / 免疫 / ヘルパーT細胞 / グルココルチコイドレセプター

Outline of Final Research Achievements

Psychological stress affects the immune system. Upon stress occurrence, glucocorticoid is released that binds to the glucocorticoid receptor and regulates gene expression. Thus, we aimed to examine the stress-induced immunomodulatory mechanisms by investigating the expression patterns of stress-inducible genes in murine immune cells.
Short restraint stress induced Rtp801, Gilz and Mkp-1 expressions in the spleen of BALB/c and C57BL/6 mice. In contrast, Bnip3 and Trp53inp1 were only upregulated upon longer restraint events. These results suggest that singular and repetitive bouts of stress lead to differential gene expression in mice and stress-induced gene expression in splenocytes.

Academic Significance and Societal Importance of the Research Achievements

社会的・心理的ストレスを受けることにより、どのように身体に影響を及ぼすのか、また、その個体差・遺伝的差異は未だ明らかにされていない。それには、ストレスの種類によっても異なると考えられる。身体への影響のなかでも、特に免疫反応への影響というのは重要であり、解明されていないことが多い。さらに、ストレスによる免疫反応への影響の個体差により、アレルギー反応を引き起こす、もしくは、免疫細胞の反応性が下がり感染症にかかりやすい、または感染症が治りにくい状態になるのではないかと予想される。ストレスによる免疫応答の機序を明らかにすることで、アレルギー、自己免疫疾患等の免疫疾患の治療法の開発に役立つと考えられる。

Research Products

• [Journal Article] Comparative Expression Analysis of Stress-Inducible Genes in Murine Immune Cells (2019)
  • Author(s): Koyanagi Madoka、Arimura Yutaka
  • Journal Title: Immunological Investigations Volume: - Issue: 8 Pages: 1-19
  • DOI: 10.1080/08820139.2019.1702673
  • Peer Reviewed
• [Journal Article] HLA Class I-Mediated HIV-1 Control in Vietnamese Infected with HIV-1 Subtype A/E (2017)
  • Author(s): Chikata Takayuki、Tran Giang Van、Murakoshi Hayato、Akahoshi Tomohiro、Qi Ying、Naranbhai Vivek、Kuse Nozomi、Tamura Yoshiko、Koyanagi Madoka、Sakai Sachiko、et al.
  • Journal Title: Journal of Virology Volume: 92 Issue: 5
  • DOI: 10.1128/jvi.01749-17
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Control of HIV-1 by an HLA-B*52:01-C*12:02 Protective Haplotype (2017)
  • Author(s): Chikata Takayuki、Murakoshi Hayato、Koyanagi Madoka、Honda Kazutaka、Gatanaga Hiroyuki、Oka Shinichi、Takiguchi Masafumi
  • Journal Title: The Journal of Infectious Diseases Volume: 216 Issue: 11 Pages: 1415-1424
  • DOI: 10.1093/infdis/jix483
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A SNP uncoupling Mina expression from the TGFβ signaling pathway (2017)
  • Author(s): Lian Shang L.、Mihi Belgacem、Koyanagi Madoka、Nakayama Toshinori、Bix Mark
  • Journal Title: Immunity, Inflammation and Disease Volume: 6 Issue: 1 Pages: 58-71
  • DOI: 10.1002/iid3.191
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] グルココルチコイドレセプター多型によるストレス感受性の相違 (2018)
  • Author(s): 石川あゆ子 工藤柚佳 戸ヶ崎萌加 小柳円 有村裕
  • Organizer: 第41回 日本分子生物学会