| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Mammalian oocyte growth is supported by surrounding granulosa/cumulus cells which provide various small molecules to the oocytes. In the present study, bovine and porcine growing oocytes collected from early antral follicles (about 0.5 mm and 1.0-1.5 mm in diameters) were cultured with surrounding cumulus cells (oocyte-cumulus complexes: OCCs) for 5-14 days. OCCs developed and formed antral-like structures in which oocytes grew to the final size and acquired maturational competence. When bovine oocytes collected from early antral follicles were denuded from cumulus cells and then cultured with parietal granulosa cells, the cells formed transzonal projections to the oocytes, and the oocytes grew to the final size. Proliferation and morphology of cumulus cells were regulated by oocyte-derived growth factors (GDF9 and BMP15) in addition to FSH during the OCC culture.
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