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Identification and functional analysis of viral factors involved in inhibition of anti-viral reaction in agricultural pests

Research Project

Project/Area Number 17K08159
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Insect science
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

TANAKA Hiromitsu  国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 主席研究員 (30391577)

Co-Investigator(Kenkyū-buntansha) 村上 理都子  国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員 (10414947)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords昆虫媒介性植物病原ウイルス / RNA干渉 / 昆虫媒介性植物ウイルス / RNA干渉反応阻害因子 / 昆虫媒介性の植物病原ウイルス / 昆虫媒介 / 植物病原ウイルス / カメムシ目昆虫 / RNA干渉阻害因子
Outline of Final Research Achievements

The viruses that infect and propagate in the insect body protect themselves from the host RNA interference-mediated antiviral response by producing factors (VSR). If the technology that inhibits VSR functions could be developed, it could become an innovative virus-transmission inhibiting technology. In this research, in order to obtain the basic knowledge indispensable for such technological development, we identified and characterized viral VSRs that functions in the host insects. First, we constructed the VSR assay system using Drosophila cell line that can identify VSR functions. Next, we found that nucleic acids derived from several phytopathogenic viruses have VSR activity using this assay system. Currently, we are in the process of analyzing which step of RNA interference reaction these VSRs inhibit. Currently, we are in the process of analyzing which step of RNA interference reaction these VSRs inhibit.

Academic Significance and Societal Importance of the Research Achievements

現在、昆虫媒介性の植物病原ウイルス病による作物被害を食い止める新規技術開発が求められている。本成果をもとに、媒介昆虫体内でのウイルスVSRの機能阻害技術が確立できれば、媒介昆虫段階でのウイルス増殖が阻害でき、その結果、媒介昆虫からの農作物へのウイルス伝播が阻止され、農作物ウイルス病の発症・まん延を効果的に食い止めることが可能となると考えられる。植物病原ウイルスを媒介する昆虫の抗ウイルス機構解明研究はまだ創成期の段階であることから、本研究成果は当該研究分野の進展にも貢献できると考えられる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (2 results)

All 2020 2018

All Presentation (2 results)

  • [Presentation] 昆虫感染ウイルス、昆虫媒介性植物病原ウイルス由来Viral Suppressor of RNA interferenceの探索と機能解析2020

    • Author(s)
      勾坂 晶、村上 理都子、渡部 賢司、田中 博光
    • Organizer
      令和2年度蚕糸・昆虫機能利用学術講演会(日本蚕糸学会第90回大会)
    • Related Report
      2019 Annual Research Report
  • [Presentation] トビイロウンカにおけるRice ragged stunt virus感染応答性遺伝子の探索・同定2018

    • Author(s)
      平成30年度蚕糸・昆虫機能利用学術講演会
    • Organizer
      村上理都子・勾坂 晶・上樂明也・渡部賢司・田中博光
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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