| Project/Area Number |
17K08161
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Insect science
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
Kasashima Megumi 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員 (90458290)
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| Co-Investigator(Kenkyū-buntansha) |
山本 大介 自治医科大学, 医学部, 准教授 (90597189)
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| Project Period (FY) |
2017-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Transgenic silkworm / Genome editing / Specific gene expression / Sterilization / apoptosis / cell death / CRISPR-Cas9 / ゲノム編集 / ノックイン系統 / カイコ / 組織特異的プロモーター / 遺伝子ノックイン / 組織特異的発現 / 不妊化 / SIT / 遺伝学 / 昆虫制御 / 有用昆虫 / 病気媒介昆虫 |
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| Outline of Final Research Achievements |
Considering the Genetic Resource Security Strategy, it is necessary to develop a practical technique for sterilizing silkworms in order to prevent the unauthorized export or dissemination of valuable strains. First, we developed an effector utilizing mammalian apoptosis-associated gene and verified its ability to induce cell death in silkworms. Next, we expressed this gene in the testes of malaria-vector mosquitoes and confirmed that sterilization of male mosquito can be achieved. In addition, to develop the site-specific knock-in technology required for inducible sterilization, we established a knock-in strain by inserting the Gal4 gene that is expressed under the native initiation codon of the silk gland expressed gene.
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| Academic Significance and Societal Importance of the Research Achievements |
The apoptosis gene used in this study can induce cell death in vivo regardless of insect type or tissue. It can be used not only as a sterilization technique, but also as a highly versatile system for functional analysis of nerves or specific tissues in Entomology.
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