| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
In this project, we investigated the molecular mechanism mediating plant responses to environmental stresses via ubiquitin signal. Ubiquitination is one of the fundamental post-translational modifications with ubiquitin, a conserved 76-amino acid protein present in eukaryotes. We previously identified a membrane localized ubiquitin ligase ATL31 regulating plant adaptation to carbon/nitrogen nutrient stress and defense response to pathogen attack. Proteomic analysis revealed that ATL31 interacts with several membrane trafficking regulators. We also found that ATL31 localized to several endosomal compartments as well as plasma membrane. In addition, we demonstrated that ATL31 physically interacts with and ubiquitinates a regulatory component of membrane trafficking system in Arabidopsis plants.
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