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Functional analysis of RNA binding PPR proteins involved in posttranscriptiona regulation of chloroplast

Research Project

Project/Area Number 17K08195
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionNagoya University

Principal Investigator

SUGITA Mamoru  名古屋大学, 情報学研究科, 招へい教員 (70154474)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords葉緑体 / 転写後制御 / PPRタンパク質 / RNAスプライシング / RNA安定性 / ヒメツリガネゴケ / シロイヌナズナ / RNA結合タンパク質 / 遺伝子発現制御 / RNA編集 / 葉緑体NDH複合体 / DYWドメイン / NDH活性 / ndhA pre-mRNA / 遺伝子 / 植物 / 発現制御 / バイオテクノロジー
Outline of Final Research Achievements

Chloroplasts are the important plant-specific organelles that are essential for plant development and growth. Chloroplasts possess their own genome and gene expression system and their gene expression is tightly regulated at the various RNA processing steps. However, regulatory factors involved in RNA processing are unknown. In this study, we identified novel pentatricopeptide repeat RNA-binding proteins required for RNA processing, such as RNA splicing and RNA stabilization, using model plants, Physcomitrium (Physcomitrella) patens and Arabidopsis thaliana.

Academic Significance and Societal Importance of the Research Achievements

学術的意義:葉緑体の遺伝子発現の仕組みを解明するため、原始的な系統に属するコケ植物を主に用いて、葉緑体遺伝子の発現制御の要となるRNA結合タンパク質ファミリーの新しい役割を明らかにした点で学術的な意義が大きい。
社会的意義:本研究の成果は、光合成機能の強化や植物の生産性を増大する基盤となるもので、農業分野や持続可能な開発目標の分野における波及効果が期待される。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (30 results)

All 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (10 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 10 results,  Open Access: 1 results) Presentation (12 results) (of which Int'l Joint Research: 1 results) Book (2 results) Remarks (5 results)

  • [Int'l Joint Research] The University of Western Australia(オーストラリア)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Moss PPR-SMR protein PpPPR_64 influences the expression of a psaA-psaB-rps14 gene cluster and processing of the 23S-4.5S rRNA precursor in chloroplasts.2021

    • Author(s)
      Ayumu Takahashi, Chieko Sugita, Mizuho Ichinose, Mamoru Sugita
    • Journal Title

      Plant Molecular Biology

      Volume: in press Issue: 4-5 Pages: 417-429

    • DOI

      10.1007/s11103-020-01090-z

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 光合成研究におけるPPR研究の幕開けと今日の隆盛:日本からの発信2020

    • Author(s)
      杉田 護
    • Journal Title

      光合成研究

      Volume: 30 Pages: 166-175

    • NAID

      40022446613

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two novel PLS-class pentatricopeptide repeat proteins are involved in the group II intron splicing of mitochondrial transcripts in the moss Physcomitrella patens.2020

    • Author(s)
      Mizuho Ichinose, Airi Ishimaru, Chieko Sugita, Kensaku Nakajima, Yasuhiro Kawaguchi, Mamoru Sugita
    • Journal Title

      Plant & Cell Physiology

      Volume: 61 Issue: 10 Pages: 1687-1698

    • DOI

      10.1093/pcp/pcaa070

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The L motifs of two moss pentatricopeptide repeat proteins are involved in RNA editing but predominantly not in RNA recognition2020

    • Author(s)
      Matsuda Takuya、Sugita Mamoru、Ichinose Mizuho
    • Journal Title

      PLOS ONE

      Volume: 15 Issue: 4 Pages: e0232366-e0232366

    • DOI

      10.1371/journal.pone.0232366

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The P-class pentatricopeptide repeat protein PpPPR_21 is needed for accumulation of the psbI-ycf12 dicistronic mRNA in Physcomitrella chloroplasts.2019

    • Author(s)
      Ebihara, T., Matsuda, T., Sugita, C., Ichinose, M., Yamamoto, H., Shikanai, T., Sugita, M.
    • Journal Title

      The Plant Journal

      Volume: 97 Issue: 6 Pages: 1120-1131

    • DOI

      10.1111/tpj.14187

    • NAID

      120006650153

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] The DYW domains of pentatricopeptide repeat protein RNA editing factors contribute to discrimination of target and non-target editing sites.2018

    • Author(s)
      Ichinose, M. and Sugita, M.
    • Journal Title

      Plant & Cell Physiol.

      Volume: 59 Issue: 8 Pages: 1652-1659

    • DOI

      10.1093/pcp/pcy086

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Light-regulated PAS-containing histidine kinases delay gametophore formation in the moss Physcomitrella patens2018

    • Author(s)
      Ryo, M., Yamashino, T., Nomoto, Y., Goto, Y., Ichinose, M., Sato, K., Sugita, M. and Aoki, S.
    • Journal Title

      J. Exp. Bot.

      Volume: 69 Issue: 20 Pages: 4839-4851

    • DOI

      10.1093/jxb/ery257

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] An evolutionarily conserved P-subfamily pentatricopeptide repeat protein is required to splice the plastid ndhA transcript in the moss Physcomitrella patens and Arabidopsis thaliana.2018

    • Author(s)
      Ito, A., Sugita, C., Ichinose, M., Kato, Y., Yamamoto, H., Shikanai, T. and Sugita, M.
    • Journal Title

      Plant J.

      Volume: 94 Issue: 4 Pages: 638-648

    • DOI

      10.1111/tpj.13884

    • NAID

      120006650169

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Chloroplast ribonucleoprotein-like proteins of the moss Physcomitrella patens are not involved in RNA stability and RNA editing.2018

    • Author(s)
      Uchiyama, H., Ichinose, M. and Sugita, M.
    • Journal Title

      Photosynthetica

      Volume: 56 Issue: SPECIAL ISSUE Pages: 62-66

    • DOI

      10.1007/s11099-017-0755-7

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] 葉緑体のndhA pre-mRNAのスプライシングに関与するペンタトリコペプチドリピート(PPR)タンパク質2018

    • Author(s)
      伊藤綾花、杉田 護
    • Journal Title

      光合成研究

      Volume: 28 Pages: 19-24

    • NAID

      40021619426

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] ヒメツリガネゴケのPAS-Histidine Kinasesの機能解析2021

    • Author(s)
      鈴木遼、山篠貴史、阿南秀、龍昌志、中井皐太、呉博文、菊地陽貴、杉田護、青木摂之
    • Organizer
      第62回日本植物生理学会年会(オンライン開催)
    • Related Report
      2020 Annual Research Report
  • [Presentation] ヒメツリガネゴケのPpPPR_32は光化学系I複合体の形成に関与する2020

    • Author(s)
      鈴木遼、杉田千恵子、一瀬瑞穂、青木摂之、 杉田護
    • Organizer
      日本植物学会第84回大会(オンライン開催)
    • Related Report
      2020 Annual Research Report
  • [Presentation] ヒメツリガネゴケの葉緑体局在型PPR遺伝子破壊株のクロロフィル蛍光測定(P77)2019

    • Author(s)
      杉田千恵子、一瀬瑞穂、杉田 護
    • Organizer
      第10回日本光合成学会年会およびシンポジウム
    • Related Report
      2019 Research-status Report
  • [Presentation] ヒメツリガネゴケのミトコンドリアP-class PPRタンパク質の機能解析2019

    • Author(s)
      一瀬瑞穂,杉田 護
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 葉緑体ndh遺伝子の転写後制御に関わるPPRタンパク質2019

    • Author(s)
      伊藤綾花,杉田千恵子,一瀬瑞穂,加藤義宣,山本宏,鹿内利治,杉田 護
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Pクラス・ぺンタトリコぺプチドタンパク質は葉緑体psbI-ycf12 mRNAの蓄積に必要である2019

    • Author(s)
      海老原哲男,松田拓也,杉田千恵子,一瀬瑞穂,山本宏,鹿内利治,杉田 護
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] PPR networks as post-transcriptional regulators in plant organelles.2018

    • Author(s)
      Mizuho Ichinose, Chieko Sugita, Airi Ishimaru, Ayaka Ito, Tetsuo Ebihara, Mamoru Sugita
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Biogenesis 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒメツリガネゴケPPR編集因子のRNA塩基認識機構2018

    • Author(s)
      松田拓也、杉田 護、一瀬瑞穂
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] ヒメツリガネゴケの新規PLS-type PPRタンパク質はミトコンドリアnad5 pre-mRNAのスプライシングに関与する2018

    • Author(s)
      一瀬瑞穂、杉田千恵子、中島健策、川口康弘、杉田 護
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 葉緑体ndhA mRNAのスプライシングに関与するPPRタンパク質2017

    • Author(s)
      伊藤綾花、杉田千恵子、加藤義宣、鹿内利治、杉田 護
    • Organizer
      日本光合成学会第8回年会およびシンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] 配列特異的なRNA結合モチーフを利用したオルガネラ遺伝子のノックダウン系の確立2017

    • Author(s)
      石丸愛理、杉田 護、一瀬瑞穂
    • Organizer
      日本農芸化学会 中部支部 第180回 例会
    • Related Report
      2017 Research-status Report
  • [Presentation] ヒメツリガネゴケPPR編集因子のRNA認識機構の解析2017

    • Author(s)
      松田拓也、杉田 護、一瀬瑞穂
    • Organizer
      日本農芸化学会 中部支部 第180回 例会
    • Related Report
      2017 Research-status Report
  • [Book] Substitutional RNA Editing in Plant Organelles. In: Picardi E., Pesole G. (eds) RNA Editing. Methods in Molecular Biology, vol 2181.2021

    • Author(s)
      Mizuho Ichinose, Mamoru Sugita
    • Total Pages
      352
    • Publisher
      Humana Press, New York, NY.
    • ISBN
      9781071607862
    • Related Report
      2020 Annual Research Report
  • [Book] Plastid Transformation in Physcomitrium (Physcomitrella) patens: An Update. In: Maliga P (ed), Chloroplast Biotechnology: Methods and Protocols, 2nd ed. Methods in Molecular Biology, vol 23172021

    • Author(s)
      Mamoru Sugita
    • Total Pages
      384
    • Publisher
      Humana Press, New York, NY.
    • ISBN
      9781071614716
    • Related Report
      2020 Annual Research Report
  • [Remarks] 名古屋大学大学院情報学研究科・青木研究室ホームページ

    • Related Report
      2020 Annual Research Report
  • [Remarks] 名古屋大学大学院理学研究科生命理学専攻ホ ームページ

    • URL

      http://www.bio.nagoya-u.ac.jp/index.php

    • Related Report
      2020 Annual Research Report
  • [Remarks] 名古屋大学大学院理学研究科生命理学専攻ホームページ、論文紹介

    • URL

      http://www.bio.nagoya-u.ac.jp/paper/index.html

    • Related Report
      2019 Research-status Report 2018 Research-status Report 2017 Research-status Report
  • [Remarks] 杉田研究室ホームページ、論文リスト

    • URL

      http://www.bio.nagoya-u.ac.jp/paper/index.html

    • Related Report
      2018 Research-status Report
  • [Remarks] 杉田研究室ホームページ、論文リスト

    • URL

      http://www.gene.nagoya-u.ac.jp/~sugita-g/ronbun.html

    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2022-01-27  

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