| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
By utilizing an original fluorometric method using Phosphate-capturing molecules, Phos-tag derivatives, we have developed convenient and reliable protocols for high-throughput analysis of phosphorylation status of unstable kinase or phosphatase substrates. We extended the fluorescent Phos-tag dye technology to profiling of histidine kinase inhibitors. A search for new antibacterial targets is essential to combat the increasing development of drug resistance among bacterial pathogens. We believe that our Phos-tag technology will result in great progress in phosphoproteomics and drug development.
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