| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
This research purpose is the development of novel assay methods for enzymes related to chromatin modification. Histone deacetylase and DNA methyltransferase are important enzymes in chromatin modification. And these assay methods will be useful for epigenetics analysis.
In terms of histone deacetylase, novel color reaction to discriminate modified amino acid and non-modified amino acid could be developed. Regarding DNA methyltransferase, novel assay method could be developed using restriction enzyme and FRET. This method could decrease the background by 1/10-1/50. Therefor, the high sensitivity measurement of the DNA methyltransferase activity was succeeded.
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