Development of derivatization procedures using reagent analogues and isotopologues for enhancing throughput of mass spectrometry-based clinical examinations
| Project/Area Number |
17K08250
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 守 千葉大学, 医学部附属病院, 特任准教授 (20401002)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 臨床検査 / 質量分析 / ハイスループット / 誘導体化 / アナログ / アイソトポログ / 多検体一括 / バイオマーカー / 分析科学 / LC/ESI-MS/MS / ハイスループット化 / 17α-ヒドロキシプロゲステロン / 副腎過形成症 / グルタル酸血症 / アナログ/アイソトポログ / 原発性アルドステロン症 / HPA軸 / 25-ヒドロキシビタミンD3 / ビタミンD欠乏症 |
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| Outline of Final Research Achievements |
Mass spectrometry (MS) is accurate and economically-efficient methodology for clinical examinations, but its throughput is poor. This disadvantage deters the introduction of MS to the clinical practice. In this study, novel sample-multiplexed methods using sets of derivatization regent analogues and/or isotopologues were designed and developed to enhance the throughput of the MS-based clinical examinations for several diseases, such as vitamin D deficiency, estrogen-dependent cancer and primary aldosteronism. The developed methods could significantly reduce the analysis time (up to 60% less, compared with the conventional assays); thus the methods had a satisfactory applicability.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により，MS臨床検査の最大の弱点であるスループットの低さが，適切にデザインされた誘導体化を基盤とする多検体一括定量，すなわち化学的アプローチによって克服できることが証明された．本成果は，各病院・検査機関が現有する装置を用いてハイスループットMS臨床検査が可能であることを意味しており，同時に検査手法がイムノアッセイから，正診度・医療経済性に優れるMSへと移行する流れを加速させるものと考えられる．
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Report
(4 results)
Research Products
(37 results)
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[Journal Article] Aldosterone and 18-oxocortisol coaccumulation in aldosterone-producing lesions2018
Author(s)
Sugiura Yuki, Takeo Emi, Shimma Shuichi, Yokota Mai, Higashi Tatsuya, Seki Tsugio, Mizuno Yosuke, Oya Mototsugu Kosaka Takeo, Omura Masao, Nishikawa Tetsuo, Suematsu Makoto, Nishimoto Koshiro
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Journal Title
Hypertension
Volume: 72
Issue: 6
Pages: 1345-1354
DOI
Related Report
Peer Reviewed / Open Access
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