Energy-resolved tandem mass spectrometry for in-situ differentiation and identification of isomers
| Project/Area Number |
17K08262
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Nakamura Takemichi 国立研究開発法人理化学研究所, 環境資源科学研究センター, 特別嘱託研究員 (10360611)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Energy-resolved MS/MS / Isomer Differentiation / Breakdown Diagram / CID / Computational Chemistry / Fragmentation Mechanism / Ion Mobility / Collision Cross Section / Protonated Amino Acid / Activation Barrier / Chemical Fingerprinting / Collision Gas Pressure / 分析科学 / 質量分析 / 異性体識別 / 衝突活性化解離 / 活性化エネルギー |
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| Outline of Final Research Achievements |
A proof of principle study of energy-resolved tandem mass spectrometry (ER-MS/MS) for differentiation and identification of isomeric small organic molecules has been carried out. The potential utility of breakdown diagrams for fingerprinting of organic molecules was shown. In the meantime, we've developed a new method for acquiring ER-MS/MS data more efficiently with a Q-TOF instrument to accelerate data accumulation.
On the other hand, we had only limited successes in our attempts to establish a method for scaling collision energy axis of breakdown diagrams and to quantitatively model fragmentation pathways and energetics based on computational chemistry. It turned out our current model on CID and fragmentation process is not good enough for quantitative evaluation. We need to refine our models and further accumulation of the experimental data will be required for future, as well.
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| Academic Significance and Societal Importance of the Research Achievements |
化学分析法として迅速かつ極めて高感度であるという特性を持つ質量分析は，有機化合物の定性，定量に広く用いられ，近年，医学や薬学等への応用との関連でも注目を集めているメタボローム解析等の新しい応用分野においても必須の分析法となっている．しかし，質量分析のみでは化合物の定性，特に異性体の識別・同定が困難であるという点が現実世界への応用展開を阻害するボトルネックの一つなっている．本研究により，クロマトグラフィーを用いることのできない局所分析等において深刻な異性体識別・同定を実現する新しい方法論の基盤を確立できたので，これを更に発展させることで，応用展開の加速が期待される．
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Report
(5 results)
Research Products
(10 results)
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[Journal Article] An integrated screening system for the selection of exemplary substrates for natural and engineered cytochrome P450s2019
Author(s)
Kanoh Naoki, Kawamata-Asano Ayano, Suzuki Kana, Takahashi Yusuke, Miyazawa Takeshi, Nakamura Takemichi, Moriya Takashi, Hirano Hiroyuki, Osada Hiroyuki, Iwabuchi Yoshiharu, Takahashi Shunji
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Journal Title
Scientific Reports
Volume: 9
Issue: 1
Pages: 18023-18023
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Increased metabolite production by deletion of an HDA1-type histone deacetylase in the phytopathogenic fungi, Magnaporthe oryzae (Pyricularia oryzae) and Fusarium asiaticum.2017
Author(s)
2.Maeda K., Izawa M., Nakajima Y., Jin Q., Hirose T., Nakamura T., Koshino H., Kanamaru K., Ohsato S., Kamakura T., Kobayashi T., Yoshida M., Kimura M.
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Journal Title
Lett. Appl. Microbiol.
Volume: 65
Issue: 5
Pages: 446-452
DOI
Related Report
Peer Reviewed / Open Access
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