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Intracellular trafficking mechanisms regulating the level of antigen presentation in dendritic cells

Research Project

Project/Area Number 17K08273
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biological pharmacy
Research InstitutionOkayama University

Principal Investigator

Furuta Kazuyuki  岡山大学, 医歯薬学総合研究科, 准教授 (50644936)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords樹状細胞 / MHC-II / エンドサイトーシス / Rab11 / 小胞輸送 / 抗原提示 / Rab / 免疫学 / 細胞生物学
Outline of Final Research Achievements

Dendritic cells induce an immune response by presenting pathogen-derived antigens to T cells by major histocompatibility antigen class II (MHC-II). The cell surface expression level of MHC-II is a regulator of the immune response. We have previously found that crosslinking of MHC-II by the TCR of activated T cells induces endocytosis of MHC-II. In this study, we analyzed the signaling mechanism induced by MHC-II crosslinking. We found that crosslinking of MHC-II induces calcium influx via activation of Syk/PLC, resulting in clathrin-dependent endocytosis of MHC-II mediated with PKC activation.

Academic Significance and Societal Importance of the Research Achievements

本研究によって、MHC-IIの細胞表面からのダウンレギュレーション機構として、カルシウム流入によるPKCの活性化を介した、クラスリン依存的エンドサイトーシスという新たなMHC-IIのエンドサイトーシスを誘導する機構が明らかとなった。今後、MHC-IIの発現異常の関連する疾患において、MHC-IIの発現調節の標的となる可能性が考えられる。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (18 results)

All 2020 2019 2018 2017 Other

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 5 results) Presentation (7 results) Remarks (4 results)

  • [Journal Article] Extracellular ATP Augments Antigen-Induced Murine Mast Cell Degranulation and Allergic Responses via P2X4 Receptor Activation2020

    • Author(s)
      Yoshida Kazuki、Ito Masa-aki、Sato Naoko、Obayashi Kosuke、Yamamoto Kimiko、Koizumi Schuichi、Tanaka Satoshi、Furuta Kazuyuki、Matsuoka Isao
    • Journal Title

      The Journal of Immunology

      Volume: 204 Issue: 12 Pages: 3077-3085

    • DOI

      10.4049/jimmunol.1900954

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ligation of MHC Class II Induces PKC-Dependent Clathrin-Mediated Endocytosis of MHC Class II.2020

    • Author(s)
      Kento Masaki, Yuhji Hiraki, Hiroka Onishi, Yuka Satoh, Paul A Roche, Satoshi Tanaka, Kazuyuki Furuta
    • Journal Title

      Cells

      Volume: 9 Issue: 8 Pages: 1810-1810

    • DOI

      10.3390/cells9081810

    • NAID

      120006885859

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Establishment and Characterization of a Murine Mucosal Mast Cell Culture Model2020

    • Author(s)
      Aya Kakinoki, Tsuyoshi Kameo, Shoko Yamashita, Kazuyuki Furuta, Satoshi Tanaka
    • Journal Title

      Int J Mol Sci

      Volume: 21 Issue: 1 Pages: 1-13

    • DOI

      10.3390/ijms21010236

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of the minimal region of peptide derived from ADP-ribosylation factor1 (ARF1) that inhibits IgE-mediated mast cell activation2019

    • Author(s)
      Uchida Ryota、Egawa Tomonori、Fujita Yoshio、Furuta Kazuyuki、Taguchi Hiroaki、Tanaka Satoshi、Nishida Keigo
    • Journal Title

      Molecular Immunology

      Volume: 105 Pages: 32-37

    • DOI

      10.1016/j.molimm.2018.11.002

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Suppression of IgE-Independent Degranulation of Murine Connective Tissue-Type Mast Cells by Dexamethasone2019

    • Author(s)
      Yamada Keiko、Sato Hitomi、Sakamaki Kazuma、Kamada Mayumi、Okuno Yasushi、Fukuishi Nobuyuki、Furuta Kazuyuki、Tanaka Satoshi
    • Journal Title

      Cells

      Volume: 8 Issue: 2 Pages: 112-112

    • DOI

      10.3390/cells8020112

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Neuromedin U directly induces degranulation of skin mast cells, presumably via MRGPRX22018

    • Author(s)
      Matsuo Yoshimi、Yanase Yuhki、Irifuku Reiko、Takahagi Shunsuke、Mihara Shoji、Ishii Kaori、Kawaguchi Tomoko、Tanaka Akio、Iwamoto Kazumasa、Watanuki Haruka、Furuta Kazuyuki、Tanaka Satoshi、Inoue Asuka、Aoki Junken、Hide Michihiro
    • Journal Title

      Allergy

      Volume: 73 Issue: 11 Pages: 2256-2260

    • DOI

      10.1111/all.13555

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Suppression of IFN-γ Production in Murine Splenocytes by Histamine Receptor Antagonists2018

    • Author(s)
      Kamei Miho、Otani Yukie、Hayashi Hidenori、Nakamura Tadaho、Yanai Kazuhiko、Furuta Kazuyuki、Tanaka Satoshi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 19 Issue: 12 Pages: 4083-4083

    • DOI

      10.3390/ijms19124083

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Rab11の活性化を介した主要組織適合抗原クラスIIの細胞表面発現制御2019

    • Author(s)
      佐藤 結香、古田 拓巳、黒田 真弘、田中 智之、古田 和幸
    • Organizer
      第92回 日本生化学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 細胞内カルシウム流入が誘導する主要組織適合抗原クラスIIのエンドサイトーシス機構の解析2019

    • Author(s)
      政木 健人、平木 勇次、田中 智之、古田 和幸
    • Organizer
      第71回日本細胞生物学会・第19回日本蛋白質科学会年会合同大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 樹状細胞におけるPKC活性化を介したMHC-IIのエンドサイトーシスの促進2019

    • Author(s)
      古田和幸、政木健人、平木勇次、田中智之
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Rab11-mediated regulation of cell-surface MHC-II on dendritic cells2018

    • Author(s)
      古田和幸、佐藤結香、黒田真弘、田中智之
    • Organizer
      第70回日本細胞生物学科 第51回日本発生生物学会合同大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Rab11による樹状細胞のMHC-II発現調節機構の解析2018

    • Author(s)
      古田和幸、佐藤結香、黒田真弘、田中智之
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 樹状細胞におけるヒスタミン産生誘導とその機能の解析2017

    • Author(s)
      古田和幸、大野恵理子、田中智之
    • Organizer
      第21回日本ヒスタミン学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 主要組織適合抗原クラスIIの細胞表面発現調節におけるRab11の役割の解析2017

    • Author(s)
      佐藤結香、黒田真弘、田中智之、古田和幸
    • Organizer
      2017年度生命科学系学会合同年次大会(ConBio 2017)
    • Related Report
      2017 Research-status Report
  • [Remarks] 分子生物学分野 _ 岡山大学大学院医歯薬学総合研究科

    • URL

      http://www.pharm.okayama-u.ac.jp/lab/bunsei/

    • Related Report
      2020 Annual Research Report
  • [Remarks] 分子生物学分野 | 岡山大学大学院医歯薬学総合研究科

    • URL

      http://www.pharm.okayama-u.ac.jp/lab/bunsei/

    • Related Report
      2019 Research-status Report
  • [Remarks] 生体応答制御学:岡山大学

    • URL

      http://www.pharm.okayama-u.ac.jp/lab/meneki/

    • Related Report
      2018 Research-status Report
  • [Remarks] 薬学部田中研究室 | 岡山大学

    • URL

      http://www.pharm.okayama-u.ac.jp/lab/meneki/

    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2022-01-27  

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