| Project/Area Number |
17K08334
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Natural medicines
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| Research Institution | Hiroshima University |
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Principal Investigator |
KUMAGAI Takanori 広島大学, 医系科学研究科(薬), 准教授 (70274058)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 抗結核薬 / D-サイクロセリン / 抗癌剤 / 異種生産 / ATP再生系 / ゲノム解析 / ヒドロキシウレア / ポリリン酸キナーゼ / ATP再生 / 培養工学 |
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| Outline of Final Research Achievements |
In the present study, production level of an anti-tubercular agent, D-cycloserine (D-CS), was improved by introducing a ATP regeneration system to the established D-CS production system using Escherichia coli. In addition, candidate genes of electron transfer system (ferredoxin and ferredoxin reductase) for DcsA, which is necessary for the synthesis of hydroxyurea (an intermediate of D-CS biosynthesis and an anti-cancer drug), were found by genome sequencing of a D-CS-producing microorganism.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で得られた成果により, 抗結核薬D-CSの大腸菌を宿主とした実用レベル高生産が達成できれば, より安全で安価なD-CSの供給につながる可能性があり, 意義があると考えられる。また, D-CS生産菌のゲノム解読は, 新規なアルギニン水酸化酵素の電子伝達系の解明, および, D-CS以外の新規化合物の発見につながる可能性があり, 学術的に意義があると考えられる。
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