Construction of high level production system for anti-tubercular agent and anti-cancer drug by Escherichia coli
| Project/Area Number |
17K08334
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Natural medicines
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| Research Institution | Hiroshima University |
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Principal Investigator |
KUMAGAI Takanori 広島大学, 医系科学研究科(薬), 准教授 (70274058)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 抗結核薬 / D-サイクロセリン / 抗癌剤 / 異種生産 / ATP再生系 / ゲノム解析 / ヒドロキシウレア / ポリリン酸キナーゼ / ATP再生 / 培養工学 |
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| Outline of Final Research Achievements |
In the present study, production level of an anti-tubercular agent, D-cycloserine (D-CS), was improved by introducing a ATP regeneration system to the established D-CS production system using Escherichia coli. In addition, candidate genes of electron transfer system (ferredoxin and ferredoxin reductase) for DcsA, which is necessary for the synthesis of hydroxyurea (an intermediate of D-CS biosynthesis and an anti-cancer drug), were found by genome sequencing of a D-CS-producing microorganism.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で得られた成果により, 抗結核薬D-CSの大腸菌を宿主とした実用レベル高生産が達成できれば, より安全で安価なD-CSの供給につながる可能性があり, 意義があると考えられる。また, D-CS生産菌のゲノム解読は, 新規なアルギニン水酸化酵素の電子伝達系の解明, および, D-CS以外の新規化合物の発見につながる可能性があり, 学術的に意義があると考えられる。
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Comprehensive analysis of resistance-nodulation-cell division superfamily (RND) efflux pumps from Serratia marcescens, Db10.2019
Author(s)
Toba S, Minato Y, Kondo Y, Hoshikawa K, Minagawa S, Komaki S, Kumagai T, Matoba Y, Morita D, Ogawa W, Gotoh N, Tsuchiya T, Kuroda T.
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Journal Title
Sci Rep.
Volume: 9
Issue: 1
Pages: 4854-4854
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Characterization of Exopolysaccharides Produced by Thermophilic Lactic Acid Bacteria Isolated from Tropical Fruits of Thailand2017
Author(s)
Panthavee, W., Noda, M., Danshiitsoodol, N., Kumagai, T., and Sugiyama, M.
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Journal Title
Biological and Pharmaceutical Bulletin
Volume: 40
Issue: 5
Pages: 621-629
DOI
NAID
ISSN
0918-6158, 1347-5215
Related Report
Peer Reviewed
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