Analysis of the Molecular basis of rotavirus host specificity and interspecies transmission

• Project/Area Number: 17K08398
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental and hygienic pharmacy
• Research Institution: Osaka Ohtani University
• Principal Investigator: Yamada Keita 大阪大谷大学, 薬学部, 講師 (80584185)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ロタウイルス / 硫酸化糖鎖 / フコシル化糖鎖 / グライコミクス / 種間伝播 / シアロ糖鎖 / ＮｅｕＡｃ / A型抗原 / B型抗原 / 糖鎖 / 宿主伝播

Outline of Final Research Achievements

We screened the binding glycans of several rotaviruses. We found that one virus bound to multiple　types of glycans. We also analyzed the glycans in several model cells used in rotavirus infection experiments and found that the expression amount of rotavirus-binding glycans differed in each cell.The observations we made in this study lead to a hypothesis that rotaviruses possess the versatile capacity to employ different glycan when they infect cells of different host species.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、ロタウイルス感染メカニズムを解明すると共に、ロタウイルス種間伝播の分子機構の理解に繋がるものである。種間伝播の機構が明らかになれば、ロタウイルス感染症の同行を予測し、感染症の予防策の開発が可能になると期待される。また、本研究では課題を進める上でいくつかの生体分子の分析法を開発している。これらの技術は、ロタウイルス感染症だけでなく、多くの疾患の原因解明やマーカー開発に適用できると期待される。

Research Products

• [Journal Article] Analysis of minor acidic N-glycans in human serum (2020)
  • Author(s): Yamada Keita、Suzuki Koji、Hirohata Yoshihiko、Kinoshita Mitsuhiro
  • Journal Title: Journal of Proteome Research Volume: - Issue: 8 Pages: 3033-3043
  • DOI: 10.1021/acs.jproteome.0c00079
  • Peer Reviewed
• [Journal Article] Screening for Components/Compounds with Anti-Rotavirus Activity: Detection of Interaction Between Viral Spike Proteins and Glycans (2020)
  • Author(s): Yamada Keita、Nio-Kobayashi Junko、Inagaki Mizuho
  • Journal Title: Methods in Molecular Biology Volume: 2132 Pages: 585-595
  • DOI: 10.1007/978-1-0716-0430-4_50
  • ISBN: 9781071604298, 9781071604304
  • Peer Reviewed
• [Journal Article] Simultaneous Analysis of Sulfated and Phosphorylated Glycans by Serotonin-Immobilized Column Enrichment and Hydrophilic Interaction Chromatography (2018)
  • Author(s): Yamada Keita、Kayahara Haruna、Kinoshita Mitsuhiro、Suzuki Shigeo
  • Journal Title: Analytical Chemistry Volume: 90 Issue: 14 Pages: 8387-8395
  • DOI: 10.1021/acs.analchem.8b00714
  • Peer Reviewed / Open Access
• [Presentation] ヒト血清中マイナー酸性糖鎖の網羅的解析 (2019)
  • Author(s): 山田佳太, 鈴木光司, 廣畑好彦
  • Organizer: 第38回日本糖質学会年会
• [Presentation] ロタウイルス増殖抑制活性を示す牛乳ラクトフォリンの分子基盤 (2018)
  • Author(s): 遠藤 江美、大野 翔平、小林 純子、山田 佳太、中川 智行、矢部 富雄、鈴木 徹、中込 とよ子、中込 治、稲垣 瑞穂
  • Organizer: 日本農芸化学会２０１８年度年大会
• [Remarks] 衛生・毒性学講座 ・研究室（講座）一覧
  • URL: https://www.osaka-ohtani.ac.jp/department/pharmacy/labo/eisei_dokuseigaku.html
• [Remarks] 薬学部薬学科教員一覧
  • URL: https://www.osaka-ohtani.ac.jp/department/teacher/pharmacy/yamadakei.html