| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Outline of Final Research Achievements |
Neuronal cells reproduce neurotransmitters in cellular pool by using extracellular TCA cycle intermediates. We demonstrated that TCA cycle intermediates, such as α-ketoglutarate and malate, are synthesized in astrocytes, and then they are transported into neuronal cells mediated by Na+-coupled citrate transporter (NaCT/Slc13a5). Transport activity of NaCT was significantly reduced by stimulation of protein kinase C activator (PMA), and reversed by addition of PKC inhibitor Go6983 in a concentration dependent manner. These results indicate that PKC-mediated regulation of NaCT might be responsible for expression level of NaCT protein in neuronal plasma membrane.
|
