Overexpression of Contactin associated protein (Caspr) 4 regulate neuronal differentiation of P19 embryonic carcinoma cells
| Project/Area Number |
17K08451
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Kagoshima University |
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Principal Investigator |
Takeda Yasuo 鹿児島大学, 医歯学域鹿児島大学病院, 教授 (60245462)
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| Co-Investigator(Kenkyū-buntansha) |
池田 龍二 宮崎大学, 医学部, 教授 (50398278)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Caspr4 / neuronal differentiation / P19 / neural differentiation / Contactin / 神経分化 / 幹細胞維持 / 神経接着関連分子 |
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| Outline of Final Research Achievements |
Contactin associated protein (Caspr) 4 is a transmembrane protein, belonging to the Caspr family. Caspr4 is one of the adhesion molecules associated with autism spectrum disorders (ASDs). We recently identified that Caspr4 regulated neuronal differentiation in cultured mouse NPCs. However the molecular function of Caspr4 has yet to be identified. In this report, we investigated the role of Caspr4 in neuronal cell differentiation of P19 embryonic carcinoma cells. We have shown that qunantitative RT-PCR revealed that Oct4 was down-regulated. Cell growth was reduced in Caspr4 overexpressing P19 cells by methylthiazolyltetrazolium (MTT) assay. Our study suggests that Caspr4 is involved in neural differentiation in P19 cells.
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| Academic Significance and Societal Importance of the Research Achievements |
マウス胚性腫瘍（EC）細胞である P19細胞は，レチノイン酸（RA）により分化誘導され，神経細胞およびアストロサイトに分化することが知られている細胞株である。Caspr4はマウス神経幹細胞において発現し、神経分化を正に調節しているが、細胞内の転写因子群の挙動を確認したデータはなかった。今回の研究で、マウス胎仔期の細胞培養より安価な株化細胞であるP19細胞株を研究に使用できたことから、今後、Caspr4の神経分化のメカニズムをさらに詳細に調べることができ、Caspr4の自閉症のメカニズムの解析、さらには、GABA性の介在性ニューロンの培養系を確立することへ貢献できるものと考えている。
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] 5-Aza-2-deoxycytidine Enhances the Sensitivity of 5-Fluorouracil by Demethylation of the Thymidine Phosphorylase Promoter2019
Author(s)
Nishizawa Y, Ikeda R, Yamamoto M, Kawahara K, Shinsato Y, Minami K, Nitta M, Terazono H, Akiyama SI, Furukawa T, Takeda Y
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Journal Title
Anticaner Research
Volume: 39(8)
Pages: 4129-4136
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Anti-proliferative Activities of Some Bivalent Symmetrical 5-Substituted Hydantoin Derivatives towards Human Brain Glioma U251 Cells (U251) and Human Carcinoma Cells (KB3-1).2019
Author(s)
Furutachi M, Ota K, Fujisaki F, Ikeda R, Yoshikawa N, Yokota T, Takeda Y, Yokomizo K, Zhou JR, Kashige N, Miake F, Sumoto K.
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Journal Title
Biol Pharm Bull.
Volume: 42(11)
Pages: 1953-1956
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Anti-proliferative Activities towards Human Brain Glioma U251 Cells and Human Carcinoma Cells (KB3-1) of Some Twin-Drug Type Bivalent C2-Symmetrical Phenylboronic Acid Derivatives.2019
Author(s)
Furutachi M, Gondo T, Ikeda R, Yoshikawa N, Yokota T, Takeda Y, Yokomizo K, Zhou JR, Kashige N, Miake F, Sumoto K.
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Journal Title
Biol Pharm Bull.
Volume: 42(5)
Pages: 833-836
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Influence of GST polymorphisms on busulfan pharmacokinetics in Japanese children.2019
Author(s)
Nishikawa T, Yamaguchi H, Ikawa K, Nakayama K, Higashi E, Miyahara E, Abematsu T, Nakagawa S, Kodama Y, Tanabe T, Shigemi A, Shinkoda Y, Okamoto Y, Takeda Y, Kawano Y.
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Journal Title
Pediatr Int.
Volume: 61(6)
Pages: 558-565
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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