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Elasticity in neuroepithelium supports cortical development

Research Project

Project/Area Number 17K08489
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionNagoya University

Principal Investigator

Shinoda Tomoyasu  名古屋大学, 医学系研究科, 助教 (80505652)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords大脳発生 / 幹細胞 / 核運動 / 神経前駆細胞 / 神経発生 / 脳発生 / 神経上皮 / 発生・分化
Outline of Final Research Achievements

Neural progenitor cells (NPCs), which are apicobasally elongated and densely packed in the developing brain, systematically move their nuclei/somata in a cell cycle-dependent manner, called interkinetic nuclear migration (IKNM). Our quantitative cell-biological and in silico analyses revealed that tissue elasticity mechanically assists an initial step of basalward IKNM. When the soma of an M-phase progenitor cell rounds up using actomyosin within the subapical space, a microzone within 10 μm from the surface, which is compressed and elastic because of the apical surface’s contractility, laterally pushes the densely neigh- boring processes of non-M-phase cells. The pressed processes then recoil centripetally and basally to propel the nuclei/somata of the progenitor’s daughter cells. Thus, indirect neighbor-assisted transfer of mechanical energy from mother to daughter helps efficient brain development.

Academic Significance and Societal Importance of the Research Achievements

「再生医療」の核となるオルガノイドの形成過程では、細胞集団が自立的に秩序立った積み重なりを産み出すことが知られていました。この「自己組織化」の原理は、実はよく判っていなかったのです。本研究で明らかになった「力の受け渡しによる細胞産生・供給方向の秩序化」は、大脳発生のみに留まらず、多細胞の積み重なりに秩序を与えて組織を形成する過程の一般的な原理のひとつであると思われます。
またヒトの脳は本研究でモデルとしたマウスよりも、はるかにたくさんの細胞が積み重なっています。もしかしたら、ヒトの脳ではより効率的で洗練された「力の受け渡し」メカニズムが存在しているのかもしれません。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (13 results)

All 2020 2019 2018 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (6 results) (of which Int'l Joint Research: 3 results) Remarks (2 results)

  • [Journal Article] Two-photon microscopic observation of cell-production dynamics in the developing mammalian neocortex in utero2020

    • Author(s)
      Kawasoe, R, Shinoda, T, Hattori, Y, Nakagawam M, Quang Pham, T, Tanaka, Y, Sagou, K, Saito, K, Katsuki, S, Kotani, T, Sano, A, Fujimori, T, Miyata, T.
    • Journal Title

      Development, Growth & Differentiation

      Volume: 62 Issue: 2 Pages: 118-128

    • DOI

      10.1111/dgd.12648

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dorsal-to-Ventral Cortical Expansion Is Physically Primed by Ventral Streaming of Early Embryonic Preplate Neurons2019

    • Author(s)
      Saito Kanako、Okamoto Mayumi、Watanabe Yuto、Noguchi Namiko、Nagasaka Arata、Nishina Yuta、Shinoda Tomoyasu、Sakakibara Akira、Miyata Takaki
    • Journal Title

      Cell Reports

      Volume: 29 Issue: 6 Pages: 1555-1567

    • DOI

      10.1016/j.celrep.2019.09.075

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lzts1 controls both neuronal delamination and outer radial glial-like cell generation during mammalian cerebral development2019

    • Author(s)
      Kawaue T.、Shitamukai A.、Nagasaka A.、Tsunekawa Y.、Shinoda T.、Saito K.、Terada R.、Bilgic M.、Miyata T.、Matsuzaki F.、Kawaguchi A.
    • Journal Title

      Nature Communications

      Volume: 10 Issue: 1 Pages: 2780-2780

    • DOI

      10.1038/s41467-019-10730-y

    • NAID

      120006649601

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Neural stem cells use external forces to streamline collective nuclear migration and secure cytogenesis2018

    • Author(s)
      篠田友靖,川上巧,宮田卓樹
    • Journal Title

      生化学

      Volume: 90 Issue: 6 Pages: 820-824

    • DOI

      10.14952/SEIKAGAKU.2018.900820

    • ISSN
      0037-1017
    • Year and Date
      2018-12-25
    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Elasticity-based boosting of neuroepithelial nukleokinesis via indirect energy transfer from mother to daughter2018

    • Author(s)
      Tomoyasu Shinoda, Arata Nagasaka, Yasuhiro Inoue, Ryo Higuchi, Yoshiaki Minami, Kagayaki Kato, Makoto Suzuki, Takefumi Kondo, Takumi Kawaue, Kanako Saito, Naoto Ueno, Yugo Fukazaawa, Masaharu Nagayama, Takashi Miura, Taiji Adachi, Takaki Miyata
    • Journal Title

      PLoS Biology

      Volume: 16 Issue: 4 Pages: 2004426-2004426

    • DOI

      10.1371/journal.pbio.2004426

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Elasticity in the subapical area maintains neuroepitherial structure in mammalian developing cerebral cortex2020

    • Author(s)
      Shinoda Tomoyasu, Miyata Takaki
    • Organizer
      第125回日本解剖学会・全国学術集会
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Novel dynamic lammelipodia-like structures in neural progenitor cells2020

    • Author(s)
      Shinoda Tomoyasu
    • Organizer
      ABiS Symposium 'Forefront and future of electron microscopic imaging'
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞間の「力」の授受が、神経系前駆細胞の秩序だった運動と大脳発生に重要である2019

    • Author(s)
      篠田 友靖
    • Organizer
      第124回日本解剖学会総会全国学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] Elasticity-based boosting of neuroepithelial nukleokinesis via indirect energy transfer from mother to daughter2018

    • Author(s)
      篠田 友靖
    • Organizer
      第70回日本細胞生物学会・第51回日本発生生物学会合同大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Elasticity-based boosting of neuroepithelial nukleokinesis via indirect energy transfer from mother to daughter2018

    • Author(s)
      篠田 友靖
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Elasticity-based boosting of neuroepithelial nucleokinesis via indirect energy transfer from mother to daughter.2018

    • Author(s)
      Tomoyasu Shinoda
    • Organizer
      Joint annual meeting of JSDB 51st and JSCB 70th
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Remarks] 研究トピックス

    • URL

      https://www.med.nagoya-u.ac.jp/medical_J/research/pdf/PLOS_B_20180423.pdf

    • Related Report
      2019 Annual Research Report
  • [Remarks] 所属研究室HP

    • URL

      https://www.takaki-miyata-lab.org/

    • Related Report
      2018 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

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