| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Epigenetic mechanism via chromatin regulation is crucial for many aspects of biological processes including maintenance of stem cells. Histone acetylation is a major epigenetic modification for these processes. Tip60 is one of the histone acetyltransferases (HATs) and is expressed in brain at relatively high levels. To investigate the role of histone acetylation via Tip60 in brain, we have produced the neural stem/progenitor cell specific Tip60 deficient mice. Although Tip60 deficient mice were born in accordance with Mendelian ratio, they died soon after delivery. The Tip60 deficient mice were microcephaly, and this was caused reduced self-renewal ability of their neural stem/progenitor cells and reduced neurogenesis. Analysis of gene expression in Tip60 deficient brain showed reduced expression in a subset of genes relating to neurogenesis. These results suggest that gene regulation controlled by Tip60 has a pivotal role in maintenance and function of neural stem/progenitor cells.
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