| Project/Area Number |
17K08525
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Meiji Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 超微細形態 / シナプス / コネクトミクス / 電子顕微鏡 / 急速凍結 / 超微形態 |
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| Outline of Final Research Achievements |
The purpose of this research is to analyze the mechanism of neural circuit reorganization morphologically, especially ultra-morphologically. First, we started the experiment of rapid freezing and substitution method and clarified the conditions under which high-sensitivity and high-resolution images can be observed. Under these conditions, we analyzed all the spines in which neural plasticity changes were distributed on a single neuron using a model of neural network reorganization. To analyze functional synaptic changes, we used the Activity Mapping method with c-Fos in combination with phosphorylation of Zif268 and CREB and FosB as a target. As a result, it was clarified that they are sensitive to input. The results of the correlation between the percentage of spines that caused morphological changes and neural activity were obtained.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果は、基礎的な脳神経回路研究の飛躍的な解明に寄与すると考えられる。さらに、これまで困難と考えられてきた微細形態学的解析と神経活動マーカーとの相関を取れる技術の革新により、脳神経回路研究だけでなく、医療分野や生命研究分野をはじめ、多くの分野に寄与できるものと示唆される。今後のさらなる検討により、飛躍的な研究へとつながる礎となった。
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