Neuronal mechanisms of taste information processing in the insular cortex

• Project/Area Number: 17K08538
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: Osaka University
• Principal Investigator: Toyoda Hiroki 大阪大学, 歯学研究科, 准教授 (00432451)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: カプサイシン受容体 / カンナビノイド受容体 / うま味受容体 / 興奮伝播 / パッチクランプ / 島皮質 / 光学的膜電位測定法 / 味覚情報処理 / カプサイシン / カンナビノイド / うま味 / オシレーション / Gタンパク質 / 情報処理 / 局所神経回路 / 味覚 / チャネル / 神経回路 / 錐体細胞 / イオンチャネル / 味覚受容 / 受容体 / 摂食 / ネットワーク / 自律神経応答

Outline of Final Research Achievements

The taste-related receptors are expressed in the central nervous system, but their physiological roles are largely unknown. In this study, the functional roles of TRPV1, CB1 and T1R1/T1R3 receptors expressed in the rat insular cortex were studied using electrophysiological methods. It is possible that when these receptors are activated, the excitation arising from the taste insular cortex propagates into the autonomic insular cortex, causing modulation of food intake and autonomic activities.

Academic Significance and Societal Importance of the Research Achievements

本研究では、島皮質味覚野に発現している味覚関連受容体（TRPV1受容体、CB1受容体、T1R1/T1R3受容体）の生理学的役割の一端を明らかにした。一般的に味覚関連受容体は、末梢神経系において中心的な役割果たすと考えられているが、中枢神経系においても重要な役割を果たしている可能性を明らかにしたため、学術的意義が高い。高次脳における味覚関連受容体の生理学的役割を明らかにすることで、健康増進に寄与することが期待される。

Research Products

• [Int'l Joint Research] ソウル大学(韓国)
• [Journal Article] Intranasal Administration of Rotenone Reduces GABAergic Inhibition in the Mouse Insular Cortex Leading to Impairment of LTD and Conditioned Taste Aversion Memory (2021)
  • Author(s): Toyoda H, Katagari A, Kato T, Sato H
  • Journal Title: International Journal of Molecular Sciences Volume: 22・1 Issue: 1 Pages: 259-259
  • DOI: 10.3390/ijms22010259
  • Peer Reviewed / Open Access
• [Journal Article] CB1 cannabinoid receptor-mediated plasticity of GABAergic synapses in the mouse insular cortex (2020)
  • Author(s): Toyoda H
  • Journal Title: Scientific Reports Volume: 10・1 Issue: 1 Pages: 7187-7187
  • DOI: 10.1038/s41598-020-64236-5
  • Peer Reviewed / Open Access
• [Journal Article] Cellular mechanisms underlying the rapid depolarization caused by oxygen and glucose deprivation in layer III pyramidal cells of the somatosensory cortex (2020)
  • Author(s): Toyoda H, Kawano T, Sato H, Kato T
  • Journal Title: Neurosci Res. Volume: - Pages: 1-9
  • DOI: 10.1016/j.neures.2020.03.003
  • Peer Reviewed / Open Access
• [Journal Article] Molecular and Regulatory Mechanisms of Desensitization and Resensitization of GABAA Receptors with a Special Reference to Propofol/Barbiturate (2020)
  • Author(s): Kang Youngnam、Saito Mitsuru、Toyoda Hiroki
  • Journal Title: International Journal of Molecular Sciences Volume: 21 Issue: 2 Pages: 563-563
  • DOI: 10.3390/ijms21020563
  • Peer Reviewed / Open Access
• [Journal Article] Interaction of nicotinic acetylcholine receptors with dopamine receptors in synaptic plasticity of the mouse insular cortex (2019)
  • Author(s): Toyoda H
  • Journal Title: Synapse Volume: 73 Issue: 7
  • DOI: 10.1002/syn.22094
  • Peer Reviewed
• [Journal Article] Role of nicotinic acetylcholine receptors for modulation of microcircuits in the agranular insular cortex (2019)
  • Author(s): Toyoda H
  • Journal Title: Journal of Oral Biosciences Volume: 61 Issue: 1 Pages: 5-11
  • DOI: 10.1016/j.job.2018.12.001
  • Peer Reviewed
• [Journal Article] Nicotinic activity layer specifically modulates synaptic potentiation in the mouse insular cortex (2018)
  • Author(s): Toyoda Hiroki
  • Journal Title: European Journal of Neuroscience Volume: - Issue: 3 Pages: 2211-2223
  • DOI: 10.1111/ejn.13857
  • Peer Reviewed
• [Journal Article] Inhibition of GluR Current in Microvilli of Sensory Neurons via Na(+)-Microdomain Coupling among GluR, HCN channel and Na(+)/K(+) Pump. (2018)
  • Author(s): Kawasaki Y, Saito M, Won J, Bae JY, Sato H, Toyoda H, Kuramoto E, Kogo M, Tanaka T, Kaneko T, Oh SB, Bae YC, Kang Y.
  • Journal Title: Front. Cell. Neurosci. Volume: 印刷中 Pages: 113-113
  • DOI: 10.3389/fncel.2018.00113
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Presynaptic mechanisms at prefrontal synapses involved in persistent pain (2018)
  • Author(s): Toyoda H
  • Journal Title: Neuroimmunology and Neuroinflammation Volume: 5 Issue: 9 Pages: 40-40
  • DOI: 10.20517/2347-8659.2018.48
  • Peer Reviewed / Open Access
• [Journal Article] Layer-dependent modulation of mouse insular synaptic activities by nicotinic acetylcholine receptors (2018)
  • Author(s): Toyoda H
  • Journal Title: Journal of Reward Deficiency Syndrome and Addiction Science Volume: 4 Issue: 01 Pages: 9-12
  • DOI: 10.17756/jrdsas.2018-041
  • Peer Reviewed / Open Access
• [Journal Article] Ion channels involved in spontaneous pain (2018)
  • Author(s): Toyoda H
  • Journal Title: Neurology and Neurobiology Volume: 2
  • DOI: 10.31487/j.nnb.2018.02.001
  • Peer Reviewed / Open Access
• [Journal Article] Electrophysiological and Morphological Properties of α and γ Motoneurons in the Rat Trigeminal Motor Nucleus (2018)
  • Author(s): Nishimura K, Ohta M, Saito M, et al.
  • Journal Title: Frontiers in Cellular Neuroscience Volume: 12 Pages: 9-9
  • DOI: 10.3389/fncel.2018.00009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Nicotine facilitates synaptic depression in layer V pyramidal neurons of the mouse insular cortex (2018)
  • Author(s): Toyoda Hiroki
  • Journal Title: Neuroscience Letters Volume: - Pages: 78-83
  • DOI: 10.1016/j.neulet.2018.02.046
  • Peer Reviewed
• [Journal Article] Nicotinic activity depresses synaptic potentiation in layer V pyramidal neurons of mouse insular cortex (2017)
  • Author(s): Sato Hajime、Kawano Tsutomu、Yin Dong Xu、Kato Takafumi、Toyoda Hiroki
  • Journal Title: Neuroscience Volume: 358 Pages: 13-27
  • DOI: 10.1016/j.neuroscience.2017.06.031
  • Peer Reviewed
• [Journal Article] A distinct functional distribution of α and γ motoneurons in the rat trigeminal motor nucleus. (2017)
  • Author(s): Morita-Isogai, Y., Sato, H., Saito, M., Kuramoto, E., Yin, D., Kaneko, T., Yamashiro, T., Takada, K., Oh, S.B., Toyoda, H., & Kang, Y.
  • Journal Title: Brain Structure and Function Volume: - Issue: 7 Pages: 1-9
  • DOI: 10.1007/s00429-017-1400-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] マウス島皮質シナプス長期増強におけるニコチンおよびドーパミン受容体の役割 (2020)
  • Author(s): 豊田博紀，佐藤元，加藤隆史
  • Organizer: 第62回歯科基礎医学会学術大会
• [Presentation] マウス島皮質シナプス可塑性におけるニコチンおよびドーパミン受容体の役割 (2020)
  • Author(s): 豊田博紀，佐藤元，加藤隆史
  • Organizer: 第43回日本神経科学大会
• [Presentation] Nicotine layer-specifically modulates synaptic plasticity in the mouse insular cortex (2019)
  • Author(s): Toyoda H, Sato H, Yin DX, Kato T
  • Organizer: FAOPS2019
  • Int'l Joint Research
• [Presentation] Nicotinic activity differentially modulates synaptic plasticity in layers III and VI pyramidal neurons of the mouse insular cortex (2019)
  • Author(s): Toyoda H, Sato H, Yin DX, Kato T
  • Organizer: Oral Neuroscience 2019
  • Int'l Joint Research
• [Presentation] マウス島皮質のシナプス長期増強におけるニコチン受容体の役割 (2019)
  • Author(s): 豊田博紀, 佐藤元, 加藤隆史
  • Organizer: 第61回歯科基礎医学会学術大会
• [Presentation] Role of intracellular Ca2+ stores in layer 2/3 pyramidal cells of the somatosensory cortex during oxygen and glucose deprivation (2018)
  • Author(s): Toyoda H, Kawano T, Shimoda M, Sato H, Yin DX, Katagiri A, Kato T
  • Organizer: Oral Neuroscience 2018
  • Int'l Joint Research
• [Presentation] ニコチンはマウス島皮質におけるシナプス可塑性を調節する (2018)
  • Author(s): 豊田博紀, 佐藤元, 尹東旭, 加藤隆史
  • Organizer: 第41回日本神経科学大会
• [Presentation] 喫煙が島皮質における味覚受容に及ぼす影響 (2018)
  • Author(s): 豊田博紀, 佐藤元, 河野奨
  • Organizer: 喫煙科学研究財団研究発表会
• [Presentation] Nicotine suppresses synaptic potentiation in layer V pyramidal neurons of the insular cortex (2017)
  • Author(s): Toyoda Hiroki、Sato Hajime、Kawano Tsutomu、Yin Dong Xu、Kato Takafumi
  • Organizer: Oral Neuroscience 2017
  • Int'l Joint Research
• [Presentation] 喫煙が島皮質における味覚受容に及ぼす影響 (2017)
  • Author(s): 豊田 博紀
  • Organizer: 喫煙科学研究財団研究発表会
• [Presentation] アナンダミドにより島皮質味覚野と胃腸自律領野間にネットワークオシレーションが誘発される (2017)
  • Author(s): 豊田 博紀、佐藤 元、尹 東旭、姜 英男
  • Organizer: 第94回日本生理学会大会
• [Book] Encyclopedia of Signaling Molecules, 2nd Edition (2018)
  • Author(s): Hiroki Toyoda
  • Total Pages: 6060
  • Publisher: Springer International Publishing
  • ISBN: 9783319672007