• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Novel anticancer strategy targeting microRNAs that regulate two types of cell death, necrosis and apoptosis

Research Project

Project/Area Number 17K08550
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General physiology
Research InstitutionTokyo University of Science

Principal Investigator

Sato Akira  東京理科大学, 薬学部薬学科, 講師 (40530663)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords細胞死 / ネクローシス / アポトーシス / 細胞死制御因子 / 非翻訳RNA / マイクロRNA / 長鎖非翻訳RNA / 制がん性非翻訳RNA / 制がん性lncRNA / 細胞死制御性非翻訳RNA / 細胞死運命 / 細胞死制御 / がん分子標的 / 核酸医薬 / 核酸化学 / 細胞死制御性miRNA / 細胞死切替え
Outline of Final Research Achievements

We aimed to find and characterize the cell death regulatory microRNAs (miRNAs) of necrosis and apoptosis as an anticancer target. (1) We identified a long noncoding RNA (lncRNA) that interact with the cell death regulatory miRNAs of necrosis and apoptosis. (2) We found that the expression patterns of noncoding RNA (i.e., miRNA and lncRNA) significantly differ in the cell death model of necrosis and apoptosis (3) The knockdown of the cell death regulating lncRNA using specific siRNA resulted in dramatic suppression of human cancer cell growth.

Academic Significance and Societal Importance of the Research Achievements

本研究から、がん細胞のネクローシスとアポトーシスを制御するmiRNAに加えて、細胞死制御性のlncRNAを同定することができた。また、細胞死制御性lncRNAのうち、その発現を特異的siRNAで阻害するとヒトがん細胞の増殖を著しく抑制する制がん性lncRNAを明らかにした。さらに、がん細胞のネクローシスとアポトーシスの制御機構におけるmiRNA、lncRNAなどの非翻訳RNAの関与の一端を明らかにすることができた。これらの研究成果は、非翻訳RNAを標的とするがんの治療戦略の構築において重要な知見であり、今後の創薬展開に繋がる点でその学術的意義、社会的意義は大きいと考える。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (30 results)

All 2020 2019 2018 2017 Other

All Journal Article (11 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 11 results,  Open Access: 9 results) Presentation (16 results) (of which Int'l Joint Research: 3 results) Remarks (3 results)

  • [Journal Article] Direct interaction analysis of microRNA-351-5p and nuclear scaffold lamin B1 mRNA by the cell-free in vitro mRNA/miRNA binding evaluation system.2020

    • Author(s)
      Akira Sato, Yoko Ogino, Akira Shimotsuma, Akiko Hiramoto, Hye-Sook Kim, Yusuke Wataya.
    • Journal Title

      Nucleosides, Nucleotides and Nucleic Acids

      Volume: 39 Issue: 6 Pages: 799-805

    • DOI

      10.1080/15257770.2019.1702675

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Low levels of brain-derived neurotrophic factor trigger self-aggregated amyloid β-induced neuronal cell death in an Alzheimer’s cell model.2020

    • Author(s)
      Nozomi Tagai, Ayako Tanaka, Akira Sato, Fumiaki Uchiumi, Sei-ichi Tanuma.
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: in press

    • NAID

      130007867921

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anticancer Non-narcotic Opium Alkaloid Papaverine Suppresses Human Glioblastoma Cell Growth2019

    • Author(s)
      Inada M, Sato A, Shindo M, Yamamoto Y, Akasaki Y, Ichimura K, Tanuma SI
    • Journal Title

      Anticancer Research

      Volume: 39(12) Issue: 12 Pages: 3743-6750

    • DOI

      10.21873/anticanres.13889

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association of ABC Transporter With Resistance to FK866, a NAMPT Inhibitor, in Human Colorectal Cancer Cells2019

    • Author(s)
      Ogino Y, Sato A, Kawano Y, Aoyama T, Uchiumi F, Tanuma SI
    • Journal Title

      Anticancer Research

      Volume: 9(12) Issue: 12 Pages: 6457-6462

    • DOI

      10.21873/anticanres.13859

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of piceatannol-rich passion fruit seed extract on human glyoxalase I-mediated cancer cell growth.2019

    • Author(s)
      Yamamoto T, Sato A, Takai Y, Yoshimori A, Umehara M, Ogino Y, Inada M, Shimada N, Nishida A, Ichida R, Takasawa R, Maruki-Uchida H, Mori S, Sai M, Morita M, Tanuma SI
    • Journal Title

      Biochem Biophys Rep.

      Volume: 20 Pages: 100684-100684

    • DOI

      10.1016/j.bbrep.2019.100684

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Anticancer effects of a non-narcotic opium alkaloid medicine, papaverine, in human glioblastoma cells,2019

    • Author(s)
      Inada M, Shindo M, Kobayashi K, Sato A, Yamamoto Y, Akasaki Y, Ichimura K, Tanuma SI,
    • Journal Title

      PLoS One,

      Volume: 14(5) Issue: 5 Pages: e0216358-e0216358

    • DOI

      10.1371/journal.pone.0216358

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] CAPZA1 determines the risk of gastric carcinogenesis by inhibiting Helicobacter pylori CagA-degraded autophagy2019

    • Author(s)
      Tsugawa H., Mori H., Matsuzaki J., Sato A., Saito Y., Imoto M., Suematsu M., Suzuki H.
    • Journal Title

      Autophagy

      Volume: 15 Issue: 2 Pages: 242-258

    • DOI

      10.1080/15548627.2018.1515530

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genomic and tumor biological aspects of the anticancer nicotinamide phosphoribosyltransferase inhibitor FK866 in resistant human colorectal cancer cells.2018

    • Author(s)
      Ogino Y, Sato A, Uchiumi F, Tanuma SI
    • Journal Title

      Genomics. pii

      Volume: S0888-7543(18) Issue: 6 Pages: 30663-30663

    • DOI

      10.1016/j.ygeno.2018.12.012

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cross resistance to diverse anticancer nicotinamide phosphoribosyltransferase inhibitors induced by FK866 treatment2018

    • Author(s)
      Ogino Yoko、Sato Akira、Uchiumi Fumiaki、Tanuma Sei-Ichi
    • Journal Title

      Oncotarget

      Volume: 9 Issue: 23 Pages: 16451-16461

    • DOI

      10.18632/oncotarget.24731

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] In vitro anticancer effects of a RAGE inhibitor discovered using a structure-based drug design system2018

    • Author(s)
      El-Far Ali、Munesue Seiichi、Harashima Ai、Sato Akira、Shindo Mika、Nakajima Shingo、Inada Mana、Tanaka Mariko、Takeuchi Akihiko、Tsuchiya Hiroyuki、Yamamoto Hiroshi、Shaheen Hazem、El-Sayed Yasser、Kawano Shuhei、Tanuma Sei-Ichi、Yamamoto Yasuhiko
    • Journal Title

      Oncology Letters

      Volume: 15 Pages: 4627-4634

    • DOI

      10.3892/ol.2018.7902

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Novel Anticancer Strategy Targeting Switch Mechanisms in Two Types of Cell Death: Necrosis and Apoptosis2017

    • Author(s)
      Sato A.
    • Journal Title

      YAKUGAKU ZASSHI

      Volume: 137 Issue: 11 Pages: 1315-1321

    • DOI

      10.1248/yakushi.17-00131

    • NAID

      130006191250

    • ISSN
      0031-6903, 1347-5231
    • Year and Date
      2017-11-01
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Shotgun proteomics of whole leakage proteins in necrosis and apoptosis2019

    • Author(s)
      Akira Sato, Yoko Ogino, Sei-ichi Tanuma.
    • Organizer
      The 78th Annual Meeting of the Japanese Cancer Association(第7 8回日本癌学会学術総会)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genomic and tumor biological features of NAMPT inhibitor FK866 resistant human cancer cells.2019

    • Author(s)
      Yoko Ogino, Akira Sato, Fumiaki Uchiumi, Sei-ichi Tanuma.
    • Organizer
      The 78th Annual Meeting of the Japanese Cancer Association(第7 8回日本癌学会学術総会)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Papaverine, an inhibitor of the HMGB1-RAGE interaction, suppresses glioblastoma.2019

    • Author(s)
      Mana Inada, Akira Sato, Mika Shindo, Koichi Ichimura, Fumiaki Uchiumi, Sei-ichi Tanuma.
    • Organizer
      The 78th Annual Meeting of the Japanese Cancer Association(第7 8回日本癌学会学術総会)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] PapaverineはHMGB1-RAGE相互作用を阻害することでヒト神経膠芽腫細胞の増殖を抑制する.2019

    • Author(s)
      稲田 愛, 佐藤 聡, 新藤 実香, 市村 幸一, 内海 文彰, 田沼 靖一.
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ドラッグリポジショニング手法による抗がん性合成過酸化物の探索.2019

    • Author(s)
      戸谷 滉希, 稲田 愛, 荻野 暢子, 綿矢 有佑, 金 惠淑, 佐藤 聡.
    • Organizer
      第63回日本薬学会関東支部大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 神経細胞保護効果を有する植物由来成分の同定及びその作用メカニズム.2019

    • Author(s)
      多賀井 希望, 田中 絢子, 佐藤 聡, 内海 文彰, 田沼 靖一.
    • Organizer
      第63回日本薬学会関東支部大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ネクローシス細胞またはアポトーシス細胞から発信されるデスシグナル分子の探索と機能解析.2019

    • Author(s)
      舟山 直輝, 下間 彰, 高橋 祐衣, 佐藤 聡.
    • Organizer
      第28回日本Cell Death学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] がん細胞のネクローシス制御因子の機能解析2019

    • Author(s)
      佐藤 聡, 高橋祐衣, 下間 彰, 田沼靖一, 綿矢有佑, 金 惠淑
    • Organizer
      日本薬学会 第139年会
    • Related Report
      2018 Research-status Report
  • [Presentation] がん細胞のネクローシスとアポトーシスの細胞死制御機構2018

    • Author(s)
      佐藤 聡, 高橋 祐衣, 下間 彰, 田沼 靖一, 金 惠淑, 綿矢 有佑
    • Organizer
      2018年度日本生化学会関東支部例会
    • Related Report
      2018 Research-status Report
  • [Presentation] がん細胞のネクローシスとアポトーシスの切替え制御機構2018

    • Author(s)
      佐藤 聡, 高橋 祐衣, 下間 彰, 田沼 靖一, 金 惠淑, 綿矢 有佑
    • Organizer
      第27回日本Cell Death 学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] ネクローシス細胞またはアポトーシス細胞から漏出する細胞死シグナル分子の探索と機能解析2018

    • Author(s)
      下間 彰, 高橋 祐依, 佐藤 聡
    • Organizer
      第62回日本薬学会関東支部大会
    • Related Report
      2018 Research-status Report
  • [Presentation] がん細胞のネクローシス抑制遺伝子の機能解析2018

    • Author(s)
      高橋 祐衣, 下間 彰, 佐藤 聡
    • Organizer
      第62回日本薬学会関東支部大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ネクローシスとアポトーシスのがん細胞死切替え機構2018

    • Author(s)
      佐藤 聡, 田沼 靖一
    • Organizer
      第77回日本癌学会学術総会
    • Related Report
      2018 Research-status Report
  • [Presentation] がん細胞のネクローシスとアポトーシスの細胞死切替え制御機構の解析.2018

    • Author(s)
      佐藤 聡, 金 惠淑, 綿矢 有佑, 田沼 靖一.
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Research-status Report
  • [Presentation] ネクローシスとアポトーシスのがん細胞死切替え制御機構の解析.2017

    • Author(s)
      佐藤 聡, 益谷 美都子, 田沼 靖一.
    • Organizer
      第76回日本癌学会学術総会
    • Related Report
      2017 Research-status Report
  • [Presentation] がん細胞のネクローシス/アポトーシスの細胞死選択における糖代謝変化.2017

    • Author(s)
      佐藤 聡, 金 惠淑, 綿矢 有佑, 田沼 靖一.
    • Organizer
      第5回がんと代謝研究会 in 札幌
    • Related Report
      2017 Research-status Report
  • [Remarks] 研究室ホームページ

    • URL

      https://pharmbiochemistrytus.com

    • Related Report
      2019 Annual Research Report
  • [Remarks] 東京理科大学薬学部佐藤聡研究室のホームページ

    • URL

      https://pharmbiochemistrytus.com/

    • Related Report
      2018 Research-status Report
  • [Remarks] 研究室ホームページ

    • URL

      http://www.tus.ac.jp/fac_grad/p/index.php?69fe

    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi