| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Induced pluripotent stem cells (iPSCs) are expected in the field of the regenerative medicine from the point of view which can differentiate into cells of all organs. However, after making them differentiate, there is a serious possibility that undifferentiated remaining iPSCs become cancer. In this research, we found that overexpression of the plasma membrane Na+/H+ exchanger (NHE1) induces the cell death of iPSCs. We found that cell death occurs through strong activation of rho kinase ROCK caused by a local intracellular pH rise triggered by NHE1. Analogy to this, we also found that Na+-ionophore monensin which is a small compound with similar function as NHE1, specifically killed iPSCs. This study provides a new simple procedure through which to eliminate the iPSCs that remain after differentiation.
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