| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
To investigate the in vivo functions of KCNQ1 channels, we identified and isolated the KCNQ1 channel ortholog and its auxiliary subunit KCNE orthologs from the zebrafish cDNA library. It turned out that the KCNE3-like gene from zebrafish was a newly-identified KCNE gene. Furthermore, we confirmed that the new KCNE gene works as a KCNQ1 modulator using Xenopus oocytes as an expression system. We are now analyzing the expression and functions of the new KCNE gene in zebrafish. We are also making transgenic zebrafish that express GFP-tagged KCNQ1 and KCNE genes. It is still a work in progress.
Another result we obtained in the project is that we found the mechanism of how KCNE3 modulates KCNQ1 channels. It has been known that KCNE3 makes the KCNQ1 channel constitutively open channel. By taking advantage of the newly-revealed KCNQ1-KCNE3 structure, we identified several critical amino acid residues in the KCNE3 and S1 segment of the KCNQ1 channel.
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