| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We performed to reveal the physiological function of the acid-activated anion channel (ASOR), which contribute to survival, and the molecular identity of ASOR. First, we created down candidate molecule A knockout cells (KO cells). As a result of a comparative experiment of KO cells and wild type cells (WT cells), it was revealed that the proliferation rate of KO cells was lower than that of WT cells and the cross-sectional area of KO cells was larger than that of WT cells. Also, we examined whether TMEM206, one of the molecular candidates for ASOR reported in a recent study, is actually candidate for ASOR in human cervix HeLa cells. It was found that the ASOR current of the TMEM206 knockdown HeLa cell was significantly suppressed compared to the ASOR current of the control HeLa cell.
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