| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Outline of Final Research Achievements |
We examined where and how blood flow controls vascular formation and found that blood flow regulates angiogenesis and vascular remodeling especially in the head region of zebrafish. We found that intracellular Ca2+ oscillations occur in endothelial cells in response to flow. These Ca2+ responses are also regulated by VEGFA/VEGFR2 signaling. Our time-lapse Ca2+ imaging in high spatial and time resolution has succeeded in visualizing endothelial responses to blood flow thereby revealed the mode of angiogenesis regulated by blood flow. In addition, we have visualized the involvement of chemokine signaling and macrophages in vascular development in the brain central arteries. Thus, our results will lead to understanding the mechanisms how blood flow regulates vascular formation in vivo.
|
