Analysisi of oxidative stress-mediated postnatal neuronal development and behavior

• Project/Area Number: 17K08564
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental physiology(including physical medicine and nutritional physiology)
• Research Institution: Tohoku University
• Principal Investigator: Nishijima Ichiko 東北大学, 東北メディカル・メガバンク機構, 講師 (70600394)
• Co-Investigator(Kenkyū-buntansha): 吉崎 嘉一 愛知県医療療育総合センター発達障害研究所, 障害モデル研究部, 主任研究員 (50393161) ; 中村 孝博 明治大学, 農学部, 専任教授 (00581985)
• Project Period (FY): 2017-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 酸化ストレス / 神経発達 / セクレチン / 概日リズム / Nrf2

Outline of Final Research Achievements

In this study, we focused on the relationship between the social behavior changes observed in mice lacking the receptor gene for the peptide hormone secretin and quantitative changes in oxidative stress. We analyzed the accumulation of oxidative stress during postnatal neurodevelopment and its effects on behavioral circadian rhythms in adult animals. We found that the activity cycle was slightly shortened in secretin receptor-deficient adult mice under constant-dark conditions. Furthermore, we analyzed the feeding behavior of adult mice deficient in secretin receptors, focusing on the feeding control output from the hypothalamus suprachiasmatic nucleus, which is the center of circadian rhythm, and found that these mice have the same feeding anticipatory activity as wild mice. Through these results, we clarify a part of the series of endocrine regulation that leads from circadian rhythm to feeding behavior.

Academic Significance and Societal Importance of the Research Achievements

視床下部摂食中枢は、脳内において酸化ストレスの影響を受ける可能性が高い領域の一つであり、本研究を通して概日リズムから摂食行動へつながる一連の内分泌制御の一端を明らかにすることができた。
本申請研究課題の成果は、学会報告並びに学術雑誌への発表を通して、社会へ発信することができた。

Research Products

• [Journal Article] Diurnal variations of triglyceride accumulation in mouse and bovine adipocyte‐derived cell lines (2022)
  • Author(s): Shiraishi Rena、Morita Satomi、Goto Yoshikuni、Mizoguchi Yasushi、Nakamura Wataru、Nakamura Takahiro J.
  • Journal Title: Animal Science Journal Volume: 93 Issue: 1
  • DOI: 10.1111/asj.13802
  • Peer Reviewed / Open Access
• [Journal Article] Oak extracts modulate circadian rhythms of clock gene expression in vitro and wheel-running activity in mice (2022)
  • Author(s): Haraguchi Atsushi、Du Yao、Shiraishi Rena、Takahashi Yuki、Nakamura Takahiro J.、Shibata Shigenobu
  • Journal Title: Sleep and Biological Rhythms Volume: 20 Issue: 2 Pages: 255-266
  • DOI: 10.1007/s41105-021-00365-2
  • Peer Reviewed / Open Access
• [Journal Article] Secretin receptor-deficient mice exhibit robust food anticipatory activity (2022)
  • Author(s): Sugiyama Mizuki、Nishijima Ichiko、Nakamura Wataru、Nakamura Takahiro J.
  • Journal Title: Neuroscience Letters Volume: 772 Pages: 136462-136462
  • DOI: 10.1016/j.neulet.2022.136462
  • Peer Reviewed / Open Access
• [Journal Article] dbTMM: an integrated database of large-scale cohort, genome and clinical data for the Tohoku Medical Megabank Project (2021)
  • Author(s): Ogishima Soichi et al.
  • Journal Title: Human Genome Variation Volume: 8 Issue: 1 Pages: 44-51
  • DOI: 10.1038/s41439-021-00175-5
  • Peer Reviewed / Open Access
• [Journal Article] Secretin receptor-deficient mice exhibit altered circadian rhythm in wheel-running activity (2020)
  • Author(s): Sugiyama Mizuki、Nishijima Ichiko、Miyazaki Shota、Nakamura Takahiro J.
  • Journal Title: Neuroscience Letters Volume: 722 Pages: 134814-134814
  • DOI: 10.1016/j.neulet.2020.134814
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Landscape of electrophilic and inflammatory stress-mediated gene regulation in human lymphoblastoid cell lines (2020)
  • Author(s): Ishida Noriko、Aoki Yuichi、Katsuoka Fumiki、Nishijima Ichiko、Nobukuni Takahiro、Anzawa Hayato、Bin Li、Tsuda Miyuki、Kumada Kazuki、Kudo Hisaaki、Terakawa Takahiro、Otsuki Akihito、Kinoshita Kengo、Yamashita Riu、Minegishi Naoko、Yamamoto Masayuki
  • Journal Title: Free Radical Biology and Medicine Volume: 161 Pages: 71-83
  • DOI: 10.1016/j.freeradbiomed.2020.09.023
  • Peer Reviewed / Open Access
• [Journal Article] Biobank Establishment and Sample Management in the Tohoku Medical Megabank Project (2019)
  • Author(s): Minegishi Naoko、Nishijima Ichiko、Nobukuni Takahiro、Kudo Hisaaki、Ishida Noriko、Terakawa Takahiro、Kumada Kazuki、Yamashita Riu、Katsuoka Fumiki、Ogishima Soichi、Suzuki Kichiya、Sasaki Makoto、Satoh Mamoru、Tohoku Medical Megabank Project Study Group、Yamamoto Masayuki
  • Journal Title: The Tohoku Journal of Experimental Medicine Volume: 248 Issue: 1 Pages: 45-55
  • DOI: 10.1620/tjem.248.45
  • NAID: 130007652672
  • ISSN: 0040-8727, 1349-3329
  • Peer Reviewed / Open Access
• [Journal Article] The risk of secondary sex ratio imbalance and increased monozygotic twinning after blastocyst transfer: data from the Japan Environment and Children’s Study (2019)
  • Author(s): Hattori Hiromitsu、Kitamura Akane、Takahashi Fumiaki、Kobayashi Norio、Sato Akiko、Miyauchi Naoko、Nishigori Hidekazu、Mizuno Satoshi、Sakurai Kasumi、Ishikuro Mami、Obara Taku、Tatsuta Nozomi、Nishijima Ichiko、Fujiwara Ikuma、Kuriyama Shinichi、Metoki Hirohito、Yaegashi Nobuo、Nakai Kunihiko、Arima Takahiro
  • Journal Title: Reproductive Biology and Endocrinology Volume: 17 Issue: 1 Pages: 27-27
  • DOI: 10.1186/s12958-019-0471-1
  • Peer Reviewed / Open Access
• [Journal Article] Association between alcohol consumption during pregnancy and hypertensive disorders of pregnancy in Japan: the Japan Environment and Children’s Study (2018)
  • Author(s): Iwama Noriyuki、Metoki Hirohito、Nishigori Hidekazu、Mizuno Satoshi、Takahashi Fumiaki、Tanaka Kosuke、Watanabe Zen、Saito Masatoshi、Sakurai Kasumi、Ishikuro Mami、Obara Taku、Tatsuta Nozomi、Nishijima Ichiko、Sugiyama Takashi、Fujiwara Ikuma、Kuriyama Shinichi、Arima Takahiro、Nakai Kunihiko、Yaegashi Nobuo
  • Journal Title: Hypertension Research Volume: 42 Issue: 1 Pages: 85-94
  • DOI: 10.1038/s41440-018-0124-3
  • Peer Reviewed
• [Journal Article] Blood pressure changes during twin pregnancies (2018)
  • Author(s): Iwama Noriyuki、Metoki Hirohito、Nishigori Hidekazu、Mizuno Satoshi、Takahashi Fumiaki、Tanaka Kosuke、Watanabe Zen、Saito Masatoshi、Sakurai Kasumi、Ishikuro Mami、Obara Taku、Tatsuta Nozomi、Nishijima Ichiko、Sugiyama Takashi、Fujiwara Ikuma、Kuriyama Shinichi、Arima Takahiro、Nakai Kunihiko、Yaegashi Nobuo
  • Journal Title: Journal of Hypertension Volume: - Issue: 1 Pages: 1-1
  • DOI: 10.1097/hjh.0000000000001846
  • Peer Reviewed
• [Journal Article] Update on the prevalence and determinants of folic acid use in Japan evaluated with 91,538 pregnant women: the Japan Environment and Children’s Study (2018)
  • Author(s): Ishikawa Tomofumi、Obara Taku、Nishigori Hidekazu、Nishigori Toshie、Metoki Hirohito、Ishikuro Mami、Tatsuta Nozomi、Mizuno Satoshi、Sakurai Kasumi、Nishijima Ichiko、Murai Yuriko、Fujiwara Ikuma、Arima Takahiro、Nakai Kunihiko、Yaegashi Nobuo、Kuriyama Shinichi、Mano Nariyasu
  • Journal Title: The Journal of Maternal-Fetal & Neonatal Medicine Volume: - Issue: 3 Pages: 1-10
  • DOI: 10.1080/14767058.2018.1494712
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Drug Use before and during Pregnancy in Japan: The Japan Environment and Children’s Study (2017)
  • Author(s): Nishigori Hidekazu、Obara Taku、Nishigori Toshie、Metoki Hirohito、Ishikuro Mami、Mizuno Satoshi、Sakurai Kasumi、Tatsuta Nozomi、Nishijima Ichiko、Fujiwara Ikuma、Arima Takahiro、Nakai Kunihiko、Mano Nariyasu、Kuriyama Shinichi、Yaegashi Nobuo
  • Journal Title: Pharmacy Volume: 5 Issue: 2 Pages: 21-21
  • DOI: 10.3390/pharmacy5020021
  • Peer Reviewed / Open Access
• [Presentation] セクレチン受容体欠損マウスにおける給餌予知活動 (2020)
  • Author(s): 杉山瑞樹、西島維知子、中村渉、中村孝博
  • Organizer: 第27回日本時間生物学会学術大会
• [Presentation] セクレチン受容体遺伝子欠損マウスにおける社会行動と概日周期 (2018)
  • Author(s): 西島維知子、杉山瑞輝、中村孝博
  • Organizer: 第29回日本行動神経内分泌研究会大会
• [Presentation] セクレチン受容体欠損マウスにおける概日リズム変化 (2018)
  • Author(s): 杉山瑞輝、西島維知子、中村孝博
  • Organizer: 第25回日本時間生物学会学術大会
• [Presentation] Quality management of TMM biobank, population-based biobank in Japan (2018)
  • Author(s): Ichiko Nishijima, Noriko Ishida, Hisaaki Kudo, Kaname Kojima, Riu Yamashita, Yosuke Kawai, Takahiro Nobukuni, Takahiro Terakawa, Atsushi Hozawa, Kozo Tanno, Soichi Ogishima, Mamoru Sato, Atsushi Shimizu, Makoto Sasaki, Masao Nagasaki, Masayuki Yamamoto, Naoko Minegishi, The Tohoku Medical Megabank Project Study Group
  • Organizer: Global Biobank Week (Joint Society of ISBER, BBMRI-ERIC,and ESBB)
  • Int'l Joint Research